##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511726_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2316251 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.924690804234945 31.0 28.0 34.0 16.0 34.0 2 26.306506937287885 31.0 25.0 34.0 10.0 34.0 3 27.95854594342323 31.0 27.0 34.0 16.0 34.0 4 32.38704829485233 35.0 32.0 37.0 22.0 37.0 5 32.82700104608698 35.0 33.0 37.0 26.0 37.0 6 33.06863548035165 36.0 35.0 37.0 26.0 37.0 7 33.10214911941754 37.0 35.0 37.0 26.0 37.0 8 33.122055424908616 37.0 35.0 37.0 26.0 37.0 9 34.67458060460632 39.0 35.0 39.0 27.0 39.0 10 34.53738735568814 39.0 35.0 39.0 27.0 39.0 11 34.54400019686985 39.0 35.0 39.0 27.0 39.0 12 34.436549838510594 39.0 35.0 39.0 27.0 39.0 13 34.43007342468498 39.0 35.0 39.0 27.0 39.0 14 35.52225600766066 40.0 36.0 41.0 27.0 41.0 15 35.457491005940206 40.0 36.0 41.0 26.0 41.0 16 35.394250018672416 40.0 36.0 41.0 26.0 41.0 17 35.36359315117403 40.0 36.0 41.0 25.0 41.0 18 35.300094851551066 40.0 36.0 41.0 25.0 41.0 19 35.33309084378161 40.0 36.0 41.0 25.0 41.0 20 35.2504940095007 40.0 36.0 41.0 25.0 41.0 21 35.157685414922646 40.0 36.0 41.0 25.0 41.0 22 35.04571913838353 40.0 35.0 41.0 24.0 41.0 23 34.92665345854141 40.0 35.0 41.0 23.0 41.0 24 34.801928202081726 39.0 35.0 41.0 22.0 41.0 25 34.68527029238196 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3385523067774727 1101 2 3.76413318802242 1101 3 2.3440333596435607 1101 4 1.5479157854720924 1101 5 1.0068688199581786 1101 6 0.8072757011219664 1101 7 0.661076108012054 1101 8 0.5588332838009364 1101 9 0.6038216732371353 1101 10 0.8650494788478866 1101 11 0.5672102080611765 1101 12 0.6857163430614861 1101 13 0.7587496529735347 1101 14 0.9459500066470028 1101 15 0.8402218876134739 1101 16 0.7581841662894462 1101 17 0.8530212172415617 1101 18 0.8393894767566721 1101 19 0.8585141137720314 1101 20 0.9759072407395166 1101 21 1.0362943352320926 1101 22 1.0824691948111038 1101 23 1.0393978407059805 1101 24 1.1298330456921946 1101 25 0.9756794077703645 1102 1 1.641878188907743 1102 2 4.276791438136581 1102 3 2.8508638636540056 1102 4 1.8145009274870532 1102 5 1.4324762853272048 1102 6 1.0344489745454695 1102 7 1.0498473771862393 1102 8 0.9632957030707772 1102 9 1.1244016052733414 1102 10 1.2399718768078003 1102 11 1.1088934388202674 1102 12 1.229748215541541 1102 13 1.140783880662319 1102 14 1.1855527404903583 1102 15 1.2097687245343849 1102 16 1.2912411695173631 1102 17 1.3888530137789203 1102 18 1.3409769324675125 1102 19 1.3758813741192242 1102 20 1.35482777085614 1102 21 1.3760227568224295 1102 22 1.1384822124873608 1102 23 1.1591900607306016 1102 24 1.0027784239410167 1102 25 1.009727207578095 1103 1 1.3158790922246482 1103 2 3.830522180494878 1103 3 2.49320353235073 1103 4 1.5924946427662334 1103 5 1.0865572405791042 1103 6 0.7313801948230321 1103 7 0.7404808149899367 1103 8 0.5588922737663253 1103 9 0.6779664416348083 1103 10 0.5587391677530675 1103 11 0.6091612028496911 1103 12 0.8600450337440861 1103 13 0.756952565813755 1103 14 0.8612411186148776 1103 15 0.4598875970977261 1103 16 0.6092288855990446 1103 17 0.7726930364008027 1103 18 0.8981589320334962 1103 19 0.7961666361899731 1103 20 0.8685504195683009 1103 21 0.9262190598226923 1103 22 1.0078546134748265 1103 23 0.7274582014858026 1103 24 0.97762418750618 1103 25 0.8288463613108519 1104 1 1.6032197921797433 1104 2 4.09425267861409 1104 3 2.6430565116670124 1104 4 1.8630040778924055 1104 5 1.3363053519951151 1104 6 1.290750615972783 1104 7 1.1049448577701995 1104 8 0.9089527336594898 1104 9 1.0978539325061263 1104 10 0.9580974902911805 1104 11 1.1269877509446573 1104 12 1.0698139993459606 1104 13 1.0620810107508092 1104 14 1.2139501397085155 1104 15 1.249053369966191 1104 16 1.007992890372158 1104 17 1.0488531334019484 1104 18 0.9851210197338389 1104 19 1.0168953254871411 1104 20 1.1304575030268325 1104 21 1.1835812043016034 1104 22 1.3286329840226685 1104 23 1.3777406682494444 1104 24 1.1981771020179437 1104 25 1.2981159540638885 1105 1 1.6198947191496984 1105 2 3.9890753254201705 1105 3 2.44717590570653 1105 4 1.7041597263390216 1105 5 1.3359826238666628 1105 6 1.0763893423236652 1105 7 1.0071473491801228 1105 8 0.8534159612225238 1105 9 0.8829221216299317 1105 10 0.7938557669229738 1105 11 0.8946427084713306 1105 12 1.0200415098698414 1105 13 0.9467959918673756 1105 14 1.0577004198544557 1105 15 1.107147122487504 1105 16 0.9897721981203205 1105 17 1.0855461690057808 1105 18 0.985521868560042 1105 19 1.034757430929595 1105 20 1.1136378606659463 1105 21 1.1086378418074787 1105 22 1.17977463477704 1105 23 1.2312867716274596 1105 24 1.0641584836785398 1105 25 1.2010330577582309 1106 1 1.320300632317192 1106 2 3.898604228448974 1106 3 2.4097203595979053 1106 4 1.5852335677951856 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1.379904949356721 1107 18 1.2462723384924104 1107 19 1.3563540922727597 1107 20 1.4516925226235529 1107 21 1.5032849714749617 1107 22 1.4761862219000932 1107 23 1.4548620153956193 1107 24 1.5676543065489454 1107 25 1.4274545555666194 1108 1 1.553715664661702 1108 2 4.071776725972246 1108 3 2.5575914666423003 1108 4 1.5960174269519314 1108 5 1.2848354507813085 1108 6 1.175891793713916 1108 7 0.9229651780457004 1108 8 0.8446488784375106 1108 9 0.8855904169018842 1108 10 0.8895714686652383 1108 11 0.7999388262350067 1108 12 0.8401133429959629 1108 13 0.8989132999185472 1108 14 0.8329734899450756 1108 15 0.8592343788934755 1108 16 0.890710827329066 1108 17 0.8579352505644877 1108 18 1.0090045634170934 1108 19 0.8030992344645398 1108 20 0.8606914664998442 1108 21 0.9948584354244119 1108 22 1.1241209266706136 1108 23 1.1209669397227913 1108 24 1.0898405227311372 1108 25 1.298753010354183 1109 1 1.1294661445038408 1109 2 3.0911630221974136 1109 3 2.00801638945763 1109 4 1.476483668415007 1109 5 0.8662395884861738 1109 6 0.7974780075039476 1109 7 0.681707626638854 1109 8 0.6432387209459662 1109 9 0.7557685509252465 1109 10 0.7080507537481893 1109 11 0.6520014454936032 1109 12 0.750679079403433 1109 13 0.670542135145979 1109 14 0.8158586524063125 1109 15 0.7730593356262574 1109 16 0.8055841928053624 1109 17 0.86129330320842 1109 18 0.7649061443972585 1109 19 0.7858215482189692 1109 20 0.8416179521012097 1109 21 0.7218846944976107 1109 22 0.7617623383105894 1109 23 0.7381556768816111 1109 24 0.7538078316955605 1109 25 0.8076024337012981 1110 1 0.9284908001670829 1110 2 3.4945663437411056 1110 3 2.053665825746407 1110 4 1.123875690268342 1110 5 0.6070676466101403 1110 6 0.6532326395197572 1110 7 0.3485943402847482 1110 8 0.30427813894591793 1110 9 0.22443490124075538 1110 10 0.27666290830401863 1110 11 0.3139341430400151 1110 12 0.3542498303357178 1110 13 0.22724736293854164 1110 14 0.2268829163966899 1110 15 0.3265510167026804 1110 16 0.4564530286653792 1110 17 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-2.3243542280540623 2315 19 -2.351154727225307 2315 20 -2.3867569761141 2315 21 -2.583558217426557 2315 22 -2.553766569471861 2315 23 -2.5712227519667437 2315 24 -2.4615383458968054 2315 25 -2.4288082801126123 2316 1 -0.6627336729764046 2316 2 1.7511299262912345 2316 3 0.40640355097246683 2316 4 -0.610346357221065 2316 5 -0.9094477195746116 2316 6 -0.949357395270205 2316 7 -1.511346019780234 2316 8 -1.3135527180443454 2316 9 -1.5389607177461073 2316 10 -1.6403559850933007 2316 11 -1.5036550386911713 2316 12 -1.7248024584580577 2316 13 -1.7105757794332561 2316 14 -1.9824183913794968 2316 15 -2.0577587845717105 2316 16 -2.115724496574032 2316 17 -2.004261886297826 2316 18 -2.272486776714061 2316 19 -2.0950421261839622 2316 20 -1.9753301812366715 2316 21 -2.228417371913828 2316 22 -1.9520509235295265 2316 23 -2.072179182498658 2316 24 -2.2130588493606282 2316 25 -2.3316643821620175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99125.0 3 1.0 4 833.0 5 4304.0 6 4998.0 7 4549.0 8 4247.0 9 4068.0 10 3971.0 11 3723.0 12 3646.0 13 3611.0 14 3769.0 15 3749.0 16 3835.0 17 4330.0 18 4972.0 19 6140.0 20 8046.0 21 10324.0 22 12646.0 23 14938.0 24 17262.0 25 19826.0 26 22320.0 27 25137.0 28 28673.0 29 34863.0 30 43045.0 31 54294.0 32 69205.0 33 89791.0 34 121975.0 35 171250.0 36 266765.0 37 342091.0 38 799929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87484387486503 18.943326953771418 13.016076409680988 25.165752761682565 2 16.10416267994411 21.328363921274114 40.31515267492535 22.25232072385643 3 18.807935754803776 24.751376254127898 29.6899170254001 26.75077096566823 4 11.92280111266007 17.167655836953767 39.08734416088757 31.822198889498594 5 12.882325792843693 37.96285463017609 36.214641677434784 12.94017789954543 6 31.00449821716213 37.87141376301618 18.511076735638753 12.613011284182932 7 27.43027418012987 31.611427258962866 22.748657205112917 18.209641355794343 8 24.31066408605976 37.83817038826967 21.002775605925265 16.848389919745312 9 26.04674536567928 15.634531836143836 20.432112063848002 37.886610734328876 10 14.383890174251409 29.578227920894584 35.03476091321709 21.003120991636916 11 34.28017947968506 22.600098175888537 24.863669783628804 18.2560525607976 12 22.900626918239865 24.844025971278587 32.5895164211478 19.665830689333756 13 28.95061890960867 22.08815020479214 26.031893780078235 22.929337105520947 14 20.506499511495086 22.390664914985468 28.26649616125368 28.836339412265772 15 22.645991302324315 31.157331394568207 23.83454988254727 22.362127420560206 16 21.704987930928038 28.542847903789355 28.19137476896934 21.560789396313265 17 21.353817008605716 28.616501406799177 28.26658250768159 21.763099076913512 18 21.036752925309045 27.930133651318446 30.27983582090197 20.753277602470543 19 22.94531119468486 27.19273515694111 27.998865407937224 21.86308824043681 20 22.954204876759903 27.74245969024946 28.686096627697083 20.617238805293553 21 22.691746936741957 27.05661711659787 27.66195084021841 22.589685106441767 22 22.44406307677363 28.34042461332556 28.050340532547573 21.165171777353237 23 22.147512793402047 27.837182183312297 28.115133494773627 21.90017152851203 24 22.58500705235059 27.092553520800223 28.955565658096344 21.366873768752843 25 22.43241341288142 27.528536415094912 28.43344697962354 21.60560319240013 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 264.0 2 456.0 3 648.0 4 648.0 5 648.0 6 1727.5 7 2807.0 8 2807.0 9 2807.0 10 4055.5 11 5304.0 12 5304.0 13 5304.0 14 8695.0 15 12086.0 16 12086.0 17 12086.0 18 21169.0 19 30252.0 20 30252.0 21 30252.0 22 47591.0 23 64930.0 24 64930.0 25 64930.0 26 91774.5 27 118619.0 28 118619.0 29 118619.0 30 153292.0 31 187965.0 32 187965.0 33 187965.0 34 210223.5 35 232482.0 36 232482.0 37 232482.0 38 248393.5 39 264305.0 40 264305.0 41 264305.0 42 276834.5 43 289364.0 44 289364.0 45 289364.0 46 293696.0 47 298028.0 48 298028.0 49 298028.0 50 285922.5 51 273817.0 52 273817.0 53 273817.0 54 242450.0 55 211083.0 56 211083.0 57 211083.0 58 179492.5 59 147902.0 60 147902.0 61 147902.0 62 119689.0 63 91476.0 64 91476.0 65 91476.0 66 70251.0 67 49026.0 68 49026.0 69 49026.0 70 35735.5 71 22445.0 72 22445.0 73 22445.0 74 15650.0 75 8855.0 76 8855.0 77 8855.0 78 5979.0 79 3103.0 80 3103.0 81 3103.0 82 2062.0 83 1021.0 84 1021.0 85 1021.0 86 679.0 87 337.0 88 337.0 89 337.0 90 213.5 91 90.0 92 90.0 93 90.0 94 61.5 95 33.0 96 33.0 97 33.0 98 21.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.341627915109373 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 3.453857116521482E-4 22 0.0011225035628694817 23 7.771178512173334E-4 24 8.634642791303705E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2316251.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.80217256831132 #Duplication Level Percentage of deduplicated Percentage of total 1 84.84703945556923 63.4674288726581 2 10.863886006268201 16.252845516066728 3 2.4616508843093974 5.524105027531432 4 0.801985324719936 2.399609786278154 5 0.3526462544319926 1.3189352989795269 6 0.17664396449397882 0.7928011389137558 7 0.10882622449875781 0.569830661743971 8 0.07142112890845285 0.4273964487706959 9 0.04708689089989353 0.3169981564919175 >10 0.2348950023150908 3.070004288653568 >50 0.01758856653449776 0.9102502589460904 >100 0.01324298837387283 1.9587631035715574 >500 0.001805784320394466 0.9186515336961887 >1k 0.0012232732492950009 1.8137300237157068 >5k 5.8251107109285764E-5 0.25864988398261707 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5936 0.25627619804589397 No Hit GTCCTACAGTGGACATTTCTAAATT 3990 0.17226112368650895 No Hit CTGTAGGACGTGGAATATGGCAAGA 3816 0.1647489844580747 No Hit TATCAACGCAGAGTACTTTTTTTTT 3577 0.15443058632246676 No Hit GGTATCAACGCAGAGTACTTTTTTT 3485 0.15045865063846708 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3333 0.14389632211707626 No Hit CTTTAGGACGTGAAATATGGCGAGG 2895 0.12498645440412115 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 4.3173213956518526E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGACC 45 7.003181E-4 14.710217 4 GTCAACG 50 0.0011106868 13.840312 2 TACGCCT 55 0.0031763625 12.035632 4 CCAACGA 190 0.0 11.945139 19 TAGGACC 660 0.0 11.892351 4 GGCGAGG 875 0.0 11.672107 19 ACTGCGC 65 8.3404843E-4 11.638853 8 TGGACCG 65 8.3404843E-4 11.638853 5 TCCAACG 350 0.0 11.61807 18 CTACGAC 60 0.006080995 11.032663 3 ATGTCGA 150 2.3028406E-9 10.7174425 17 GCCGGTT 230 0.0 10.690033 11 CCCGTTT 240 0.0 10.638639 12 TTAGGAC 1380 0.0 10.621508 3 GTCCTAC 2080 0.0 10.551336 1 AGGACGT 2275 0.0 10.516535 5 CGGTTTC 270 0.0 10.5072975 13 GGACGTG 2230 0.0 10.474315 6 GACGTGA 1180 0.0 10.418252 7 GGTATCA 1160 0.0 10.397279 1 >>END_MODULE