FastQCFastQC Report
Thu 26 May 2016
SRR1511726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316251
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73050.31538032795236787No Hit
TATCAACGCAGAGTACTTTTTTTTT47070.20321631809333274No Hit
GTCCTACAGTGGACATTTCTAAATT44940.19402042352059426No Hit
GGTATCAACGCAGAGTACTTTTTTT43790.18905550391559464No Hit
CTGTAGGACGTGGAATATGGCAAGA42810.1848245289478558No Hit
GTCCTAAAGTGTGTATTTCTCATTT36380.1570641523738144No Hit
CTTTAGGACGTGAAATATGGCGAGG32070.13845649715855493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC902.3010216E-914.77299210
ACCGACC400.00529149414.2453868
CCAACGA2350.013.33610619
AACCGAT500.001503599113.2956937
TCCAACG3050.013.07773218
GCGTGCG1102.763045E-912.9503519
TAGGACC5600.012.384734
GGTATCA13450.012.129931
GCGCCAC1657.2759576E-1211.51142313
GGCGTGC1900.011.4962778
TACGACC600.005874311.0840514
AGTGCGA600.00589131711.07974410
AAGGCGT2200.010.7919596
GTATCAA28500.010.5785621
CCGCTCA909.55008E-510.5521379
TTATACT2800.010.51853754
CGTTTCC2550.010.42799514
GGCGAGG9900.010.36028119
CTAGACA2400.010.2923344
TTAGGAC13900.010.2524623
Produced by FastQC (version 0.11.5)