FastQCFastQC Report
Thu 26 May 2016
SRR1511725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593304
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58220.36540421664666634No Hit
TATCAACGCAGAGTACTTTTTTTTT36280.2277029367904681No Hit
GGTATCAACGCAGAGTACTTTTTTT35020.2197948414113063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG655.6912497E-513.0939265
TGCGATA905.7128455E-712.60896710
TCCAACG1207.9489837E-1012.60896618
GGTATCA9900.012.3805551
AGCGTCG854.149948E-612.23811417
ATAAGCG854.149948E-612.23811414
GCGATAA854.149948E-612.23811411
ACCGTGC701.1384237E-412.1586478
CGTCGGT803.00354E-511.82090719
CCGGTTT803.00354E-511.82090712
TAATCTG1800.011.558225
CGACTAA1002.0383923E-611.3480717
GTATCAA22750.011.2096471
GCGTCGG855.576142E-511.12555918
CGATAAG1053.6605925E-610.80768612
CTGCGAT1159.3488416E-710.6902119
TTAAGCG1159.3488416E-710.69021112
CGCCCTA803.933234E-410.63881616
TCCCGTT909.965135E-510.50747211
CCCGTTT1002.5223826E-510.40239812
Produced by FastQC (version 0.11.5)