##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511725_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1593304 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.783634510426133 31.0 28.0 34.0 16.0 34.0 2 26.17880203652285 31.0 25.0 34.0 10.0 34.0 3 27.809307577210628 31.0 26.0 34.0 16.0 34.0 4 32.239450224188225 35.0 32.0 37.0 20.0 37.0 5 32.682516330844585 35.0 33.0 37.0 25.0 37.0 6 32.922517611202885 36.0 35.0 37.0 26.0 37.0 7 32.943625321972455 37.0 35.0 37.0 26.0 37.0 8 32.958662628098594 37.0 35.0 37.0 26.0 37.0 9 34.49178060181861 39.0 35.0 39.0 27.0 39.0 10 34.35783001862796 39.0 35.0 39.0 26.0 39.0 11 34.363442255840695 39.0 35.0 39.0 26.0 39.0 12 34.26193808588945 39.0 35.0 39.0 25.0 39.0 13 34.24163750294984 39.0 35.0 39.0 25.0 39.0 14 35.316705412149844 40.0 36.0 41.0 25.0 41.0 15 35.22467024497522 40.0 36.0 41.0 25.0 41.0 16 35.16149523254821 40.0 35.0 41.0 25.0 41.0 17 35.13422548364907 40.0 36.0 41.0 25.0 41.0 18 35.068910264456754 40.0 35.0 41.0 24.0 41.0 19 35.09938781299739 40.0 35.0 41.0 24.0 41.0 20 35.01634653525002 40.0 35.0 41.0 23.0 41.0 21 34.92842608817903 40.0 35.0 41.0 22.0 41.0 22 34.812951577351214 39.0 35.0 41.0 21.0 41.0 23 34.68607434613859 39.0 35.0 41.0 19.0 41.0 24 34.55134550594237 39.0 35.0 41.0 18.0 41.0 25 34.42746770233427 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2654395052820497 1101 2 3.714632321686281 1101 3 2.4214550705739093 1101 4 1.4572213634674895 1101 5 1.0897046739894733 1101 6 0.7678475409876384 1101 7 0.8111577445155902 1101 8 0.5714917192178532 1101 9 0.7971059810191932 1101 10 0.8409074142328876 1101 11 0.6311450218099921 1101 12 0.8538958729303232 1101 13 0.623655264170516 1101 14 0.8930011783265996 1101 15 0.6457235682849003 1101 16 0.7665958207400152 1101 17 0.8580303739298216 1101 18 0.7824636805944749 1101 19 0.7546963886212055 1101 20 0.977029512144874 1101 21 0.9078373734928462 1101 22 0.8839742613612103 1101 23 0.7892288774522243 1101 24 1.0309863518451863 1101 25 0.8475787223147293 1102 1 1.7049883460534758 1102 2 4.364844672866397 1102 3 2.808106884261857 1102 4 1.91460491843511 1102 5 1.5607956716919063 1102 6 1.1787338460771082 1102 7 1.0672017464650452 1102 8 1.0944409638046366 1102 9 1.2423611505520142 1102 10 1.2063658839119213 1102 11 1.203178719505459 1102 12 1.2642836749215505 1102 13 1.2120170270334256 1102 14 1.4002127636500035 1102 15 1.3898578020800727 1102 16 1.5611707702630966 1102 17 1.6525273453146312 1102 18 1.448165831955606 1102 19 1.371656633695352 1102 20 1.4778496750634886 1102 21 1.34723861278826 1102 22 1.2865809591262973 1102 23 1.2057045440956884 1102 24 1.1323245123966004 1102 25 1.1944115566808051 1103 1 1.820142205015646 1103 2 4.3139978980231035 1103 3 2.9837730514038796 1103 4 2.018914258471746 1103 5 1.533253601511376 1103 6 1.2703171243553086 1103 7 1.209581847121946 1103 8 1.1168390043789316 1103 9 1.381742491963685 1103 10 1.0112037532513156 1103 11 1.1942012410843859 1103 12 1.441273313813575 1103 13 1.400760534555836 1103 14 1.4824071270783534 1103 15 1.3787850057625874 1103 16 1.3019137191772998 1103 17 1.4672643966152776 1103 18 1.5323980410899267 1103 19 1.4980267008894756 1103 20 1.2813952258028394 1103 21 1.2731950950605082 1103 22 1.4958876941150479 1103 23 1.5300319314743618 1103 24 1.6088210721220264 1103 25 1.1930655257532479 1104 1 1.776566325064465 1104 2 4.199548493442208 1104 3 2.938571273863971 1104 4 1.9924528765804297 1104 5 1.587834699742352 1104 6 1.459545654195189 1104 7 1.2015314240236563 1104 8 1.2568719683375207 1104 9 1.1753001261813267 1104 10 1.2251177469180163 1104 11 1.2367097456235214 1104 12 1.1872379657458012 1104 13 1.1801675887618828 1104 14 1.3641747737436418 1104 15 1.3377270370398264 1104 16 1.1611651170032218 1104 17 1.2673692082179429 1104 18 1.2124063935812828 1104 19 1.414327439233496 1104 20 1.4039528316382288 1104 21 1.4738109899326446 1104 22 1.5769190240668394 1104 23 1.4321263389543049 1104 24 1.2782418309012655 1104 25 1.3097976743302837 1105 1 1.4224277381893309 1105 2 3.819115989773117 1105 3 2.307809300640862 1105 4 1.59958289724716 1105 5 0.981562048710515 1105 6 0.9400564068833646 1105 7 0.7133118325659069 1105 8 0.6034627072478784 1105 9 0.6822601705099558 1105 10 0.5577977571016248 1105 11 0.733860582775705 1105 12 0.8914922413620587 1105 13 0.5798497255217043 1105 14 0.6519233734980361 1105 15 0.7060552785709646 1105 16 0.9445482451668781 1105 17 0.9093425062081764 1105 18 0.6989355815885858 1105 19 0.7099814362982215 1105 20 0.7779176233236171 1105 21 0.826304101023787 1105 22 0.7913606480522191 1105 23 0.9801540143968381 1105 24 0.9703244528798791 1105 25 0.8315445368815801 1106 1 1.5615561706958658 1106 2 4.176979160745141 1106 3 2.6441927989058662 1106 4 1.700174080899643 1106 5 1.2213652016899914 1106 6 1.145203024713119 1106 7 1.0994506107863558 1106 8 0.871312812204394 1106 9 0.9553409656091318 1106 10 1.0192942442712223 1106 11 1.135596649262446 1106 12 0.9690277980599973 1106 13 1.1772777553379186 1106 14 1.1291494155663244 1106 15 1.2974863285645384 1106 16 1.2421487035484091 1106 17 1.213195374305677 1106 18 1.220927185067147 1106 19 1.3988217738726547 1106 20 1.317725595735041 1106 21 1.234073560819013 1106 22 1.2399571223700008 1106 23 1.2839774304102036 1106 24 1.3066324467110064 1106 25 1.2407501873977935 1107 1 1.5263991181420984 1107 2 3.897700096918591 1107 3 2.6155151493404176 1107 4 1.7459984233660677 1107 5 1.3159277427943366 1107 6 0.9916056814803156 1107 7 0.9677172441166064 1107 8 0.8974141367832971 1107 9 1.0477250445729496 1107 10 0.8654110050589026 1107 11 1.0552218320238964 1107 12 0.9628690768738011 1107 13 0.9670262647110661 1107 14 1.1139268134780878 1107 15 1.0734308451774837 1107 16 1.0989631918435236 1107 17 1.1427192977116434 1107 18 1.0692468667768722 1107 19 0.9526577261073683 1107 20 1.1220999129273892 1107 21 1.216245826734891 1107 22 1.2493226615774304 1107 23 1.2711697253505037 1107 24 1.3231910417985944 1107 25 1.4483324096683248 1108 1 1.6462271953351717 1108 2 4.109417691412116 1108 3 2.6415393350912204 1108 4 1.6228316706055494 1108 5 1.3631076171414591 1108 6 1.1789631299233463 1108 7 1.027915392436455 1108 8 0.9751847032808669 1108 9 1.0456618825884618 1108 10 0.9969628578428242 1108 11 0.9065116945827825 1108 12 1.023401887462903 1108 13 1.0264738512133178 1108 14 1.1143555167280184 1108 15 1.1172751345058245 1108 16 1.1235061217719533 1108 17 1.079379827431687 1108 18 1.1234736335773121 1108 19 1.112319884988061 1108 20 1.0977304952309126 1108 21 1.1127027334488844 1108 22 1.266435029511662 1108 23 1.3366160668031455 1108 24 1.1563133348090986 1108 25 1.249754945066151 1109 1 1.088823902277376 1109 2 3.089766881336132 1109 3 1.9852286554795704 1109 4 1.264614750821842 1109 5 0.8934380388679273 1109 6 0.6517086635995781 1109 7 0.6854252076965182 1109 8 0.5040096112152455 1109 9 0.4685083587325636 1109 10 0.5191306353838883 1109 11 0.3809575255066804 1109 12 0.44805213956544776 1109 13 0.4262080529562269 1109 14 0.5315248667396872 1109 15 0.5496213620981152 1109 16 0.617280152673537 1109 17 0.5902501645490901 1109 18 0.4039293457036024 1109 19 0.6693234661630498 1109 20 0.7372187828611487 1109 21 0.817867538513255 1109 22 0.8104875398848961 1109 23 0.6079879713186713 1109 24 0.652816363083133 1109 25 0.723833528016506 1110 1 1.1432016816752046 1110 2 3.645592345704202 1110 3 2.2777623152102926 1110 4 1.3494940592481868 1110 5 0.7141119625047523 1110 6 0.6388602196786621 1110 7 0.5930292948262093 1110 8 0.3622757657756708 1110 9 0.4509638595901251 1110 10 0.4296596370452903 1110 11 0.31100322369908895 1110 12 0.3920705109294502 1110 13 0.2999532711449504 1110 14 0.5020577821001879 1110 15 0.6913229770745346 1110 16 0.7877006451089699 1110 17 0.7312231408405196 1110 18 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2315 23 -2.9836520516615046 2315 24 -2.8372918560778366 2315 25 -2.890425111406987 2316 1 -0.9888696823011713 2316 2 1.4615774932910988 2316 3 -0.11990816641193192 2316 4 -1.1336879744794146 2316 5 -1.0417907199905407 2316 6 -1.355864811479318 2316 7 -1.817483094622446 2316 8 -1.7598902679378305 2316 9 -2.0715268870321495 2316 10 -2.043254217665954 2316 11 -2.0320316373988803 2316 12 -2.163495588948855 2316 13 -2.0539494242777465 2316 14 -2.3923253191960185 2316 15 -2.3643127545078784 2316 16 -2.5073682550215395 2316 17 -2.262886663362181 2316 18 -2.47370494412133 2316 19 -2.5313062019819697 2316 20 -2.261642577276227 2316 21 -2.435631679668866 2316 22 -2.2220056841093125 2316 23 -2.5080028003709387 2316 24 -2.57689632825155 2316 25 -2.5957796414385683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72365.0 3 4.0 4 616.0 5 3089.0 6 3589.0 7 3392.0 8 3143.0 9 2935.0 10 2878.0 11 2777.0 12 2759.0 13 2770.0 14 2664.0 15 2782.0 16 2996.0 17 3237.0 18 3710.0 19 4386.0 20 5866.0 21 7419.0 22 9010.0 23 10694.0 24 12235.0 25 14208.0 26 15772.0 27 17909.0 28 20372.0 29 24488.0 30 30112.0 31 37817.0 32 48693.0 33 62843.0 34 84576.0 35 118247.0 36 182622.0 37 233009.0 38 537320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62256041533819 18.705344366172433 12.840110863965695 24.83198435452368 2 16.961835656571424 21.246788040522677 38.806899325037506 22.984476977868393 3 18.679423386874067 24.993535445840845 29.544958149857152 26.782083017427937 4 12.490585600739093 16.73673071805506 38.018984449922925 32.753699231282916 5 13.558868866205067 37.44018718336237 35.21882829642052 13.782115654012038 6 31.700165191325695 36.84394189683827 18.140605935841496 13.315286975994537 7 27.80787596089635 31.787593579128654 22.318904615817196 18.0856258441578 8 25.555010217761332 35.818086190708115 20.70169911077861 17.925204480751948 9 26.643440297645647 15.349173792320864 20.08963763349618 37.91774827653731 10 15.399760497682802 28.626301069977856 33.741771815045965 22.232166617293373 11 34.31284927421258 22.901153828773417 23.91075400551307 18.875242891500932 12 23.175489423236243 24.85137801700115 31.29622470037105 20.67690785939155 13 28.880301562037126 21.728621782158335 25.89612528431486 23.494951371489684 14 21.80876970119952 21.450143852020705 27.6353414037748 29.105745043004976 15 23.662402153010348 29.1741563443009 23.821631025843153 23.3418104768456 16 22.940882593654443 27.96101685554044 26.421448763073464 22.676651787731657 17 22.10896351229897 27.814089464408546 27.360440945356316 22.716506077936163 18 21.997497025049835 27.271757304318573 28.828647891425618 21.902097779205974 19 23.350346198842153 26.637728895427365 27.907919643708922 22.104005262021563 20 23.656439700145107 26.591974915019357 28.00695912393366 21.744626260901875 21 23.725143523147285 26.333947782491272 27.339535566815226 22.601373127546214 22 23.33758451098486 26.781540516317243 27.69820069742745 22.182674275270447 23 23.13932411635783 26.65004280445769 27.56368575251743 22.646947326667053 24 23.082286870553265 26.430612111687413 27.943129496944714 22.54397152081461 25 23.16682817591621 26.491868469545043 27.792059770138028 22.549243584400717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 353.0 1 353.0 2 507.5 3 662.0 4 662.0 5 662.0 6 1573.0 7 2484.0 8 2484.0 9 2484.0 10 3347.0 11 4210.0 12 4210.0 13 4210.0 14 6164.0 15 8118.0 16 8118.0 17 8118.0 18 13695.5 19 19273.0 20 19273.0 21 19273.0 22 29869.0 23 40465.0 24 40465.0 25 40465.0 26 56496.5 27 72528.0 28 72528.0 29 72528.0 30 91251.0 31 109974.0 32 109974.0 33 109974.0 34 124770.5 35 139567.0 36 139567.0 37 139567.0 38 152149.0 39 164731.0 40 164731.0 41 164731.0 42 176059.5 43 187388.0 44 187388.0 45 187388.0 46 194895.5 47 202403.0 48 202403.0 49 202403.0 50 198970.5 51 195538.0 52 195538.0 53 195538.0 54 179885.5 55 164233.0 56 164233.0 57 164233.0 58 143051.0 59 121869.0 60 121869.0 61 121869.0 62 100877.5 63 79886.0 64 79886.0 65 79886.0 66 62329.5 67 44773.0 68 44773.0 69 44773.0 70 33073.5 71 21374.0 72 21374.0 73 21374.0 74 15082.5 75 8791.0 76 8791.0 77 8791.0 78 5965.0 79 3139.0 80 3139.0 81 3139.0 82 2119.0 83 1099.0 84 1099.0 85 1099.0 86 717.0 87 335.0 88 335.0 89 335.0 90 209.5 91 84.0 92 84.0 93 84.0 94 52.0 95 20.0 96 20.0 97 20.0 98 13.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.32579093506324 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 4.3933863217565514E-4 22 0.001255253234787586 23 7.531519408725517E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1593304.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.41985132643514 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20531953467449 61.92127509339005 2 11.721993458779206 17.446980209035875 3 2.916155910571881 6.510596679283934 4 0.9780102553664529 2.911335112004012 5 0.4142792162958235 1.5415298842183625 6 0.23601429117979508 1.0538489076308593 7 0.12558868858228558 0.6542404072802958 8 0.07875668547513404 0.46888486592178313 9 0.05705815544759913 0.3821633500833857 >10 0.24029404573618868 3.0969788466627177 >50 0.014063716391781791 0.7272019874018052 >100 0.011014635654760485 1.6592993419193118 >500 8.537681438660701E-4 0.46318690485310615 >1k 5.12260886319642E-4 0.792564259415315 >5k 8.537681438660701E-5 0.36991415089914736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5822 0.36540421664666634 No Hit TATCAACGCAGAGTACTTTTTTTTT 3628 0.2277029367904681 No Hit GGTATCAACGCAGAGTACTTTTTTT 3502 0.2197948414113063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.27626617393793E-5 0.0 6 0.0 0.0 0.0 6.27626617393793E-5 0.0 7 0.0 0.0 0.0 6.27626617393793E-5 0.0 8 0.0 0.0 0.0 6.27626617393793E-5 0.0 9 0.0 0.0 0.0 6.27626617393793E-5 0.0 10 0.0 0.0 0.0 6.27626617393793E-5 0.0 11 0.0 0.0 0.0 6.27626617393793E-5 0.0 12 0.0 0.0 0.0 6.27626617393793E-5 6.27626617393793E-5 13 0.0 0.0 0.0 6.27626617393793E-5 6.27626617393793E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACCG 65 5.6912497E-5 13.093926 5 TGCGATA 90 5.7128455E-7 12.608967 10 TCCAACG 120 7.9489837E-10 12.608966 18 GGTATCA 990 0.0 12.380555 1 AGCGTCG 85 4.149948E-6 12.238114 17 ATAAGCG 85 4.149948E-6 12.238114 14 GCGATAA 85 4.149948E-6 12.238114 11 ACCGTGC 70 1.1384237E-4 12.158647 8 CGTCGGT 80 3.00354E-5 11.820907 19 CCGGTTT 80 3.00354E-5 11.820907 12 TAATCTG 180 0.0 11.55822 5 CGACTAA 100 2.0383923E-6 11.34807 17 GTATCAA 2275 0.0 11.209647 1 GCGTCGG 85 5.576142E-5 11.125559 18 CGATAAG 105 3.6605925E-6 10.807686 12 CTGCGAT 115 9.3488416E-7 10.690211 9 TTAAGCG 115 9.3488416E-7 10.690211 12 CGCCCTA 80 3.933234E-4 10.638816 16 TCCCGTT 90 9.965135E-5 10.507472 11 CCCGTTT 100 2.5223826E-5 10.402398 12 >>END_MODULE