FastQCFastQC Report
Thu 26 May 2016
SRR1511725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593304
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76700.48138961554103926No Hit
TATCAACGCAGAGTACTTTTTTTTT51090.32065443882648886No Hit
GGTATCAACGCAGAGTACTTTTTTT48980.30741151719947984No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23260.14598595120579627No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19000.11924905730482067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTC500.001503501913.29538619
TAGGACC2750.012.7834024
GGTATCA14950.012.5094151
CCCGTAC701.0944725E-412.21004916
CCCCGTA855.3416617E-511.17259515
TGCTCGA600.005890961311.07948910
CGTCCAA600.005890961311.07948910
GGCGAGG2950.010.94535419
GATAGTC700.001495760710.8533767
TTAGGAC4400.010.7967923
CCTATAC1355.675065E-810.5568643
CTAGGGT909.5025476E-510.5568644
GGACCGG909.548018E-510.5518946
TGGACCG750.002644911910.1345895
TCCGGGG750.00265526310.12981914
GCCTTAC856.333361E-410.1028911
GTATAAG1909.094947E-1110.043811
TGGCGAG6650.09.996531518
TAGGACG5950.09.9003874
CTTATCC1356.429327E-79.8530733
Produced by FastQC (version 0.11.5)