FastQCFastQC Report
Thu 26 May 2016
SRR1511724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919483
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64040.333631503899748No Hit
TATCAACGCAGAGTACTTTTTTTTT38330.19968918714049566No Hit
GGTATCAACGCAGAGTACTTTTTTT37070.19312491957469796No Hit
GTCCTACAGTGGACATTTCTAAATT21540.1122177169581601No Hit
CTGTAGGACGTGGAATATGGCAAGA21380.11138415917202706No Hit
GTCCTAAAGTGTGTATTTCTCATTT21020.10950865415322772No Hit
CTTTAGGACGTGAAATATGGCGAGG19820.10325697075722995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.012.56679251
ACCGCTC658.3381275E-411.6390558
GCCTTAT1155.286456E-711.1736771
CCTACAC2350.010.8651813
TAGGACC4200.010.8076944
GGCCTAA1103.7632708E-610.78298951
ACACCGT1106.3848365E-610.3164356
GCTATAT1156.401142E-610.3141631
ACTGTTC7800.010.3054148
GGTCTAC1751.2951205E-910.166821
GGACGTG14050.010.1634646
AGGACGT14150.010.1584695
GATATAC6350.010.1178971
TATAGCG750.002750230310.0871815
GAACAGT5350.010.07539756
GTAGGAC12450.010.0264163
TCCAACG2550.010.01301118
TTAGGAC8550.09.9544553
TAAAGCG951.7217691E-49.9544545
CCAACGA1901.09139364E-109.95445419
Produced by FastQC (version 0.11.5)