##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511724_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1919483 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.89831428566963 31.0 28.0 34.0 16.0 34.0 2 26.266314940012492 31.0 25.0 34.0 10.0 34.0 3 27.916542110557895 31.0 27.0 34.0 16.0 34.0 4 32.3648122958109 35.0 32.0 37.0 22.0 37.0 5 32.80204669694913 35.0 33.0 37.0 26.0 37.0 6 33.05086525903069 36.0 35.0 37.0 26.0 37.0 7 33.082006977920614 37.0 35.0 37.0 26.0 37.0 8 33.0971798135227 37.0 35.0 37.0 26.0 37.0 9 34.64135759472733 39.0 35.0 39.0 27.0 39.0 10 34.508934437033304 39.0 35.0 39.0 27.0 39.0 11 34.51293864024844 39.0 35.0 39.0 27.0 39.0 12 34.41181297255563 39.0 35.0 39.0 27.0 39.0 13 34.391911780411704 39.0 35.0 39.0 26.0 39.0 14 35.47719828724714 40.0 36.0 41.0 26.0 41.0 15 35.39520798048225 40.0 36.0 41.0 26.0 41.0 16 35.33363462974145 40.0 36.0 41.0 25.0 41.0 17 35.3032483225952 40.0 36.0 41.0 25.0 41.0 18 35.23917846628493 40.0 36.0 41.0 25.0 41.0 19 35.26546158522894 40.0 36.0 41.0 25.0 41.0 20 35.18911394370255 40.0 36.0 41.0 25.0 41.0 21 35.09199143727764 40.0 35.0 41.0 24.0 41.0 22 34.977105293456624 40.0 35.0 41.0 24.0 41.0 23 34.856195131709946 39.0 35.0 41.0 23.0 41.0 24 34.730637885305576 39.0 35.0 41.0 21.0 41.0 25 34.60293474857553 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.301464002827089 1101 2 3.774323960519826 1101 3 2.3865190936571388 1101 4 1.473255719581644 1101 5 1.0312736182252138 1101 6 0.8398365101936136 1101 7 0.6530805971256299 1101 8 0.36308004534153326 1101 9 0.4288510298864665 1101 10 0.7108002710525909 1101 11 0.5011580924382883 1101 12 0.3889041049337294 1101 13 0.45405476276111756 1101 14 0.6265136541622667 1101 15 0.6033870974040951 1101 16 0.5041198118291845 1101 17 0.7038603656324085 1101 18 0.6420156368616716 1101 19 0.7785620226283001 1101 20 0.7392617096423137 1101 21 0.7321625479144629 1101 22 0.6717815854267499 1101 23 0.6700726090818918 1101 24 0.7413234253918404 1101 25 0.663935235082505 1102 1 1.5762796890606445 1102 2 4.2040068505775885 1102 3 2.6988595931524806 1102 4 1.7674878531006755 1102 5 1.2483379063000086 1102 6 1.0279178641230473 1102 7 0.9500404177316213 1102 8 0.9909053629624793 1102 9 1.0424351577539284 1102 10 1.0758575289829508 1102 11 1.1422563137908597 1102 12 1.195784754356751 1102 13 1.2426554701022638 1102 14 1.5024454802616773 1102 15 1.5009874936703014 1102 16 1.423062037678541 1102 17 1.3701455862904695 1102 18 1.3659541589051045 1102 19 1.2190941174308136 1102 20 1.3267942438288571 1102 21 1.2784687008574025 1102 22 1.0804837017061146 1102 23 1.0387482578820126 1102 24 0.9409249958353882 1102 25 1.0304460410202267 1103 1 1.729856939814475 1103 2 4.279518966018109 1103 3 3.0569213543669242 1103 4 1.990915104886291 1103 5 1.5275912891780479 1103 6 1.389217239733739 1103 7 1.3308690196636164 1103 8 1.1163758633102034 1103 9 1.1997543275952793 1103 10 1.2133781238380266 1103 11 1.3152520103666987 1103 12 1.533491503893984 1103 13 1.643706943573406 1103 14 1.7130276245449494 1103 15 1.3119202980569185 1103 16 1.401612254008242 1103 17 1.5093561268015634 1103 18 1.6008967901736568 1103 19 1.6838751503612173 1103 20 1.6156330361186804 1103 21 1.6251106579216312 1103 22 1.7127695559414207 1103 23 1.457236437132103 1103 24 1.7003970000465003 1103 25 1.3515686601898196 1104 1 1.6941111275048257 1104 2 4.19400415408688 1104 3 2.7204176372840045 1104 4 1.9377675975274968 1104 5 1.5024070218337044 1104 6 1.3562362568618838 1104 7 1.2148469106869086 1104 8 1.2835485242351794 1104 9 1.3923251291336527 1104 10 1.1388960679777753 1104 11 1.4001903993421507 1104 12 1.324162351898046 1104 13 1.359925746966269 1104 14 1.4588178569527557 1104 15 1.6536987508769911 1104 16 1.3357672286487343 1104 17 1.4483080718927042 1104 18 1.4123744026442608 1104 19 1.5612895467228896 1104 20 1.5916895786917067 1104 21 1.5227398727342205 1104 22 1.7669519339569035 1104 23 1.5004402173076343 1104 24 1.5779155733876067 1104 25 1.4668770533702968 1105 1 1.485187400954139 1105 2 3.8102369661575537 1105 3 2.3807366586839365 1105 4 1.615000506784014 1105 5 1.0824708803569507 1105 6 0.8076748171682837 1105 7 0.7342245080269194 1105 8 0.702963542966863 1105 9 0.804317861828352 1105 10 0.7520286194547907 1105 11 0.8011891856492142 1105 12 0.8323196663917471 1105 13 0.7741853958896812 1105 14 0.8444451366629622 1105 15 0.8417117008094976 1105 16 0.8337390796257083 1105 17 0.8353860359236123 1105 18 0.8858037616148522 1105 19 0.8788334635834687 1105 20 0.8873382683456725 1105 21 0.9046150041046133 1105 22 0.906117859890962 1105 23 1.060244864082307 1105 24 0.9972382494271699 1105 25 0.901676493760668 1106 1 1.3779942369198501 1106 2 3.9341717878715343 1106 3 2.3900117962398646 1106 4 1.587601183531298 1106 5 1.0205731570211114 1106 6 0.9042349325201329 1106 7 0.7226274184674182 1106 8 0.710125972089223 1106 9 0.5879791971281421 1106 10 0.6842286345869439 1106 11 0.7571702031304639 1106 12 0.662357413497368 1106 13 0.8944544729843571 1106 14 0.8584554055264704 1106 15 1.0424801822116194 1106 16 0.981296703965441 1106 17 0.8705465579609779 1106 18 0.9642241247067531 1106 19 0.9630181612174624 1106 20 1.0804762376911299 1106 21 1.0589763025876664 1106 22 0.9096149064071142 1106 23 0.991701304313402 1106 24 0.9611556800319718 1106 25 1.0494654444693268 1107 1 1.5299991892689135 1107 2 3.8990518931456677 1107 3 2.497664071427156 1107 4 1.790715053780339 1107 5 1.3504463081514757 1107 6 1.1765693365125642 1107 7 0.9417993392944553 1107 8 0.7578628278762878 1107 9 1.030069244110841 1107 10 0.9893789601172429 1107 11 1.0224384774013089 1107 12 0.8257823623509992 1107 13 0.8720818836195647 1107 14 1.041454763718484 1107 15 0.9994313152156735 1107 16 1.0621894779711951 1107 17 0.9859959924502988 1107 18 1.083054800485037 1107 19 1.1630125802784264 1107 20 1.1824851554442404 1107 21 1.0262114172686267 1107 22 1.1063397029598505 1107 23 1.1766958482111605 1107 24 1.3766848632027973 1107 25 1.3760513490734567 1108 1 1.5524736998849207 1108 2 4.046956708416154 1108 3 2.649787273161774 1108 4 1.5502438808423307 1108 5 1.2907859229785572 1108 6 1.1233686982120972 1108 7 0.9026175576565549 1108 8 0.8988163805543508 1108 9 0.9900006633548628 1108 10 0.8331610121801418 1108 11 0.8635266335169334 1108 12 0.8096817203961422 1108 13 0.8637114988120942 1108 14 0.9754728895026545 1108 15 1.0401704768981546 1108 16 1.0318750784486994 1108 17 1.1348314595424966 1108 18 1.098805229876092 1108 19 0.980980007464666 1108 20 1.0152583516972697 1108 21 0.9064636921276232 1108 22 1.0980436241224396 1108 23 1.0280004661444764 1108 24 1.0374214668108976 1108 25 1.0054369239702154 1109 1 1.1780195484055866 1109 2 2.933401481632451 1109 3 1.9722477172241817 1109 4 1.234603616024863 1109 5 0.8711287640632861 1109 6 0.6396252725012204 1109 7 0.5889339445457225 1109 8 0.48071721451446336 1109 9 0.4272522592299737 1109 10 0.4020862863786263 1109 11 0.457403487335597 1109 12 0.4765591500247268 1109 13 0.4999968911480366 1109 14 0.4925270569614 1109 15 0.3526256036727702 1109 16 0.40518835486226124 1109 17 0.5281140444606862 1109 18 0.4897264430993289 1109 19 0.633125794822746 1109 20 0.599456364188768 1109 21 0.5375108672151683 1109 22 0.6099956748598316 1109 23 0.5344732308861779 1109 24 0.6080714157043232 1109 25 0.5907984031129487 1110 1 1.156681836404573 1110 2 3.590971061916836 1110 3 2.185038889813555 1110 4 1.4132131402490344 1110 5 0.8684885020895905 1110 6 0.583887963536668 1110 7 0.4392991445038419 1110 8 0.4693126200232456 1110 9 0.4350458004878277 1110 10 0.4583877738195028 1110 11 0.47017615632221066 1110 12 0.5050813911479253 1110 13 0.43556185226973554 1110 14 0.5287223326858168 1110 15 0.5312727052414772 1110 16 0.541346882535251 1110 17 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2315 19 -2.4268246365916895 2315 20 -2.423446855544185 2315 21 -2.466383501718198 2315 22 -2.2819162245104607 2315 23 -2.1815812312279164 2315 24 -2.3214540284189056 2315 25 -2.2807721473961706 2316 1 -0.9287186443387654 2316 2 1.4290216688838626 2316 3 -0.0023502463007787355 2316 4 -1.035445559720447 2316 5 -1.1941215458705159 2316 6 -1.3510999036502334 2316 7 -1.850340967049501 2316 8 -1.6923511990632107 2316 9 -2.0337568777062387 2316 10 -2.0207893978887697 2316 11 -1.7815670686398875 2316 12 -1.9484838771855166 2316 13 -1.9396145916329317 2316 14 -2.054581627327302 2316 15 -2.1985902519012157 2316 16 -2.4684319853190786 2316 17 -2.3009917792863206 2316 18 -2.603902395974515 2316 19 -2.440431618455335 2316 20 -2.2017190652372065 2316 21 -2.4861142990334457 2316 22 -2.3287377143806722 2316 23 -2.5325244914607694 2316 24 -2.621515259837288 2316 25 -2.4371156953964856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82175.0 3 4.0 4 669.0 5 3512.0 6 4225.0 7 3868.0 8 3547.0 9 3361.0 10 3264.0 11 3170.0 12 3067.0 13 3139.0 14 3191.0 15 3346.0 16 3342.0 17 3561.0 18 4259.0 19 5153.0 20 6723.0 21 8732.0 22 10679.0 23 12327.0 24 14606.0 25 16620.0 26 18832.0 27 21088.0 28 24307.0 29 29294.0 30 36032.0 31 45488.0 32 57910.0 33 75802.0 34 102529.0 35 144208.0 36 222583.0 37 282760.0 38 652110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.653317065063874 18.19635808183766 13.13400535456683 25.016319498531637 2 16.89449652442162 20.845138682792552 39.13400512419781 23.126359668588016 3 18.95166563079746 24.312379948142286 29.511696639147107 27.224257781913153 4 12.704097926368716 16.782748271279296 37.595331659618765 32.91782214273323 5 13.760945004462139 37.27394303570284 35.129198851982544 13.835913107852479 6 32.0425343699319 36.4761761370119 18.005160764643396 13.476128728412807 7 28.12830329833606 31.46753578958501 21.920642172918438 18.48351873916049 8 25.649354539738045 36.005684864101426 20.416226661033203 17.928733935127322 9 26.659626576531288 15.404512569269954 19.895565628869857 38.040295225328904 10 15.793002594969582 28.43130155359542 33.528924194692 22.246771656742986 11 34.659749526304736 22.372222103556012 24.00552648812206 18.96250188201719 12 23.691379397473174 24.240381394365045 31.118275077195268 20.949964130966514 13 29.20088377964275 21.58529145608479 25.596319425595333 23.617505338677134 14 21.75268027901263 21.612694668303913 27.29542277790426 29.339202274779197 15 23.69638074418997 29.56619047941555 23.637041849289623 23.10038692710485 16 22.93758267200074 27.504281100692218 26.84879209662185 22.70934413068519 17 22.250939445673655 27.660364796145632 27.358877364373633 22.729818393807083 18 22.12533270677573 27.02248470030732 29.020262226860044 21.8319203660569 19 23.718038218684224 26.459040990268196 27.362462750328216 22.46045804071936 20 23.85491301564015 26.61128022493557 27.75002435551656 21.78378240390772 21 23.760249526043264 26.227950396956672 27.056248075649698 22.955552001350366 22 23.693449678373586 26.840058912310372 27.250017322553234 22.21647408676281 23 23.253946918735984 26.784553252144338 27.31292772179016 22.648572107329514 24 23.585934337527345 26.244462701675396 27.73376997868697 22.435832982110288 25 23.450481197280727 26.559026571217352 27.64932015547937 22.341172076022552 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 323.0 1 323.0 2 492.5 3 662.0 4 662.0 5 662.0 6 1613.0 7 2564.0 8 2564.0 9 2564.0 10 3285.5 11 4007.0 12 4007.0 13 4007.0 14 5939.5 15 7872.0 16 7872.0 17 7872.0 18 14170.5 19 20469.0 20 20469.0 21 20469.0 22 32222.0 23 43975.0 24 43975.0 25 43975.0 26 63568.5 27 83162.0 28 83162.0 29 83162.0 30 107793.0 31 132424.0 32 132424.0 33 132424.0 34 148914.5 35 165405.0 36 165405.0 37 165405.0 38 180149.0 39 194893.0 40 194893.0 41 194893.0 42 210203.5 43 225514.0 44 225514.0 45 225514.0 46 237304.0 47 249094.0 48 249094.0 49 249094.0 50 245709.5 51 242325.0 52 242325.0 53 242325.0 54 222449.5 55 202574.0 56 202574.0 57 202574.0 58 176146.0 59 149718.0 60 149718.0 61 149718.0 62 124346.5 63 98975.0 64 98975.0 65 98975.0 66 76604.0 67 54233.0 68 54233.0 69 54233.0 70 40112.0 71 25991.0 72 25991.0 73 25991.0 74 18088.0 75 10185.0 76 10185.0 77 10185.0 78 6889.0 79 3593.0 80 3593.0 81 3593.0 82 2329.0 83 1065.0 84 1065.0 85 1065.0 86 702.0 87 339.0 88 339.0 89 339.0 90 214.5 91 90.0 92 90.0 93 90.0 94 57.5 95 25.0 96 25.0 97 25.0 98 15.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.329186557005194 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.5629208489994443E-4 20 0.0 21 2.0838944653325925E-4 22 0.001041947232666296 23 5.730709779664629E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1919483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.26748718941484 #Duplication Level Percentage of deduplicated Percentage of total 1 79.17011591997706 54.04744874351521 2 13.756670387967846 18.782666389591945 3 3.8573001530014084 7.8998456634225445 4 1.4590754390609373 3.9842965537792936 5 0.6496145865490204 2.217377773264613 6 0.34759884192811796 1.4237819693029934 7 0.19866627582612778 0.9493713207950252 8 0.12048931560087042 0.6580402247395026 9 0.08031929934363977 0.4934877065104233 >10 0.3200963629460318 3.7323543382523034 >50 0.020739816606327975 0.9921263745122132 >100 0.016533320223817916 2.3689015763399146 >500 0.0017762906190234827 0.8537962414326574 >1k 9.267603229687721E-4 1.2588670406747893 >5k 7.723002691406435E-5 0.337638083866539 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6404 0.333631503899748 No Hit TATCAACGCAGAGTACTTTTTTTTT 3833 0.19968918714049566 No Hit GGTATCAACGCAGAGTACTTTTTTT 3707 0.19312491957469796 No Hit GTCCTACAGTGGACATTTCTAAATT 2154 0.1122177169581601 No Hit CTGTAGGACGTGGAATATGGCAAGA 2138 0.11138415917202706 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2102 0.10950865415322772 No Hit CTTTAGGACGTGAAATATGGCGAGG 1982 0.10325697075722995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2097361633314805E-5 2 0.0 0.0 0.0 0.0 5.2097361633314805E-5 3 0.0 0.0 0.0 0.0 5.2097361633314805E-5 4 0.0 0.0 0.0 0.0 5.2097361633314805E-5 5 0.0 0.0 0.0 0.0 5.2097361633314805E-5 6 0.0 0.0 0.0 0.0 5.2097361633314805E-5 7 0.0 0.0 0.0 0.0 5.2097361633314805E-5 8 0.0 0.0 0.0 5.2097361633314805E-5 5.2097361633314805E-5 9 0.0 0.0 0.0 1.0419472326662961E-4 5.2097361633314805E-5 10 0.0 0.0 0.0 1.562920848999444E-4 5.2097361633314805E-5 11 0.0 0.0 0.0 1.562920848999444E-4 5.2097361633314805E-5 12 0.0 0.0 0.0 1.562920848999444E-4 5.2097361633314805E-5 13 0.0 0.0 0.0 1.562920848999444E-4 5.2097361633314805E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1345 0.0 12.5667925 1 ACCGCTC 65 8.3381275E-4 11.639055 8 GCCTTAT 115 5.286456E-7 11.173677 1 CCTACAC 235 0.0 10.865181 3 TAGGACC 420 0.0 10.807694 4 GGCCTAA 110 3.7632708E-6 10.7829895 1 ACACCGT 110 6.3848365E-6 10.316435 6 GCTATAT 115 6.401142E-6 10.314163 1 ACTGTTC 780 0.0 10.305414 8 GGTCTAC 175 1.2951205E-9 10.16682 1 GGACGTG 1405 0.0 10.163464 6 AGGACGT 1415 0.0 10.158469 5 GATATAC 635 0.0 10.117897 1 TATAGCG 75 0.0027502303 10.087181 5 GAACAGT 535 0.0 10.0753975 6 GTAGGAC 1245 0.0 10.026416 3 TCCAACG 255 0.0 10.013011 18 TTAGGAC 855 0.0 9.954455 3 TAAAGCG 95 1.7217691E-4 9.954454 5 CCAACGA 190 1.09139364E-10 9.954454 19 >>END_MODULE