FastQCFastQC Report
Thu 26 May 2016
SRR1511724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919483
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83790.4365237931255447No Hit
TATCAACGCAGAGTACTTTTTTTTT54110.2818988237978664No Hit
GGTATCAACGCAGAGTACTTTTTTT52460.27330275912836943No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24680.12857628851102093No Hit
GTCCTACAGTGGACATTTCTAAATT24120.12565883625955532No Hit
GTCCTAAAGTGTGTATTTCTCATTT23740.12367913651748935No Hit
CTGTAGGACGTGGAATATGGCAAGA23740.12367913651748935No Hit
CTTTAGGACGTGAAATATGGCGAGG22420.11680228478189178No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19740.10284019186416343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.012.4345731
GTCTAGG1501.2732926E-1112.080851
TAGGACC3500.011.9431524
TAAGACT1657.2759576E-1211.5154364
GTACCGT752.0798537E-411.3967186
TGTAGAA7800.010.59641552
GTCCTAA7500.010.1733471
ACTGCGG750.002654388810.1304168
GCGATAA750.002654388810.13041611
GTCTAGA1701.4570105E-910.0985431
GCGTCGG856.6119264E-410.05592718
GATATAC5900.010.0224711
GTATCAA31600.010.0204251
AATGTCC3650.09.8875648
TAAGCCC1252.5693626E-69.8802444
GGCGAGG5700.09.830502519
GTAGGAC11900.09.6599033
TGTAGGA13200.09.6441772
GTATCAT1304.0311916E-69.5375131
CGGTAAG900.00111351269.5002352
Produced by FastQC (version 0.11.5)