FastQCFastQC Report
Thu 26 May 2016
SRR1511723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476345
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45790.3101578560566805No Hit
GGTATCAACGCAGAGTACTTTTTTT28790.19500861925904853No Hit
TATCAACGCAGAGTACTTTTTTTTT27810.1883706044318909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCA500.001551931313.23944959
GACGCTC500.001551931313.23944958
CGTCTAA450.00885411213.1789911
ACCGACC604.2523476E-412.6098
TTCAACG655.749964E-412.1652222
CTTAAGC803.003108E-511.8209383
GCATAAT958.756671E-611.4449141
GTATAGC1950.011.1514541
CCGTTCA600.00607835411.0328759
TCTTATA1353.173409E-810.9824932
CTTACAC2500.010.96983053
TCTACAC2000.010.8752623
GGTATCA10400.010.8346511
GCCGGTT700.001549174410.80771411
GTCTTAT1302.2255335E-710.6445691
GTGCTAC1552.1957021E-810.203091
CCTACAC2053.6379788E-1210.1487073
AGTACCG750.002749544610.08725
CTTATAC1901.09139364E-109.95447353
TTCTAGG2700.09.8842442
Produced by FastQC (version 0.11.5)