FastQCFastQC Report
Thu 26 May 2016
SRR1511723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476345
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56720.38419204183304034No Hit
GGTATCAACGCAGAGTACTTTTTTT35530.24066190490705086No Hit
TATCAACGCAGAGTACTTTTTTTTT33200.2248796859812578No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17760.12029708503093789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.012.6361121
ACCCCGC600.00588892611.0799168
TAGAACC1303.259629E-810.9626144
TTATACT2350.010.9159644
GTCTTAT1053.3056385E-610.89994051
TCTAGAC1158.8023626E-710.7401843
CCTATCC803.767017E-410.6885483
CGAACCG909.543208E-510.552312
ACCGAGC909.543208E-510.55238
GTATAGG1451.3566023E-810.524081
GTATAAG2001.8189894E-1210.4911921
ATTATAC1451.4400939E-810.4837873
TAGACAG2100.010.4057835
TAGAAGT2100.010.4057834
TAGGACC1659.258656E-1010.3646544
GTAGAAC1951.4551915E-1110.2317733
GTCTATG1409.1407856E-810.2186951
TAGACTG2151.8189894E-1210.1637885
AGACCGT856.611244E-410.0557216
GAATAGG951.579707E-410.0394191
Produced by FastQC (version 0.11.5)