FastQCFastQC Report
Thu 26 May 2016
SRR1511722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099120
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48660.23181142574031025No Hit
GGTATCAACGCAGAGTACTTTTTTT28810.1372479896337513No Hit
TATCAACGCAGAGTACTTTTTTTTT28110.1339132588894394No Hit
GTCCTACAGTGGACATTTCTAAATT23720.11299973322154044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.011.7254991
GTCCTAC11450.011.3950071
CCGTGTA1002.03936E-611.3480929
GTCCCGA802.6130714E-411.11984351
TATCGCG600.00607983411.0328677
GTCCTAT2350.010.9358271
TAGGACC5300.010.8841774
GTATTAG2001.8189894E-1210.8727351
TCGTCAA700.001549654610.80770710
TGGCGAG11600.010.76112218
TCCTACA12850.010.6150512
TTCAACG854.5772718E-410.46573452
ATGGCGA12350.010.41390317
GTAGGAC12350.010.4139033
TCCAACG1651.0059011E-910.31644718
CCTACAG13400.010.3036173
TATGGCG12400.010.29564816
GGTCTAG1251.5470196E-610.2796771
AATCCCG2600.010.18418519
TTAGGAC8400.010.1322253
Produced by FastQC (version 0.11.5)