FastQCFastQC Report
Thu 26 May 2016
SRR1511722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099120
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59270.28235641602195205No Hit
TATCAACGCAGAGTACTTTTTTTTT36600.17435877891687945No Hit
GGTATCAACGCAGAGTACTTTTTTT36380.17331072068295286No Hit
GTCCTACAGTGGACATTTCTAAATT25610.1220035062311826No Hit
CTGTAGGACGTGGAATATGGCAAGA22940.10928389039216434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCC400.005291307314.24537314
GCGTGCG400.005291307314.2453739
CCAACGA2000.013.29568119
TCCAACG2650.012.54309618
CGAATTT550.00307653612.08698315
GGTATCA12000.012.0072011
TAGGACC5050.011.2874974
CCCGTTT2100.010.85361812
GTCCTAC13850.010.816731
AGGACGT12900.010.6786435
GCTAGAC1252.2399945E-710.6403473
TTAGGAC7250.010.614143
CGTTTCC2600.010.59271314
GTAGGAC14100.010.5109813
GGACGTG12650.010.4353466
TAGGACG13450.010.3126044
TCCTACA15100.010.2553012
GTATCAA25200.010.1858631
TGTAGGA14900.010.1379152
TCCCGTT2250.010.13004211
Produced by FastQC (version 0.11.5)