FastQCFastQC Report
Thu 26 May 2016
SRR1511721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914672
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46100.24077230982643502No Hit
TATCAACGCAGAGTACTTTTTTTTT27190.1420086573575004No Hit
GGTATCAACGCAGAGTACTTTTTTT26830.1401284397536497No Hit
GTCCTACAGTGGACATTTCTAAATT23350.12195300291642643No Hit
GTCCTAAAGTGTGTATTTCTCATTT19810.10346419647856134No Hit
CTGTAGGACGTGGAATATGGCAAGA19380.10121838100729523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1051.4679244E-913.50959219
TCCAACG1600.012.41193818
CGTTAGG655.7411974E-412.167791
TAGGACC4300.011.6556464
GCATAAT853.5451165E-511.6309771
GGTATCA10950.011.4663371
TCGCGCG600.00608082311.0325469
ATCGCGC700.001549924110.8073918
GTATAGA2300.010.3161711
GAACAGT4100.010.1484046
CTAGTAC750.002750863610.0868993
ATTATAC2250.010.0868993
GTATCGC856.8787596E-410.012736
TCTGCGA951.7222506E-49.9541768
TTAGGAC8000.09.9292913
GCCTAAT1001.8451015E-49.886331
GTAGGAC12250.09.8038473
TAAGACC1554.3624823E-89.7615154
TCTATAC1554.3624823E-89.7615153
ACTGGTA1857.039489E-109.7120488
Produced by FastQC (version 0.11.5)