FastQCFastQC Report
Thu 26 May 2016
SRR1511721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914672
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56960.29749220754259736No Hit
TATCAACGCAGAGTACTTTTTTTTT35990.18796953211829495No Hit
GGTATCAACGCAGAGTACTTTTTTT35030.18295561850802644No Hit
GTCCTACAGTGGACATTTCTAAATT25280.13203305840373702No Hit
CTGTAGGACGTGGAATATGGCAAGA22080.11532001303617538No Hit
GTCCTAAAGTGTGTATTTCTCATTT21610.11286528449781477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1301.4551915E-1113.14941718
TATCGCG550.003076643512.0868387
TCCCGTT1302.6302587E-911.68837111
CCCGTTT1251.8415449E-811.39616112
TCGCGCG600.005891249511.0796029
GGTATCA11750.011.0462741
CCCCGTA951.3656287E-510.99629615
CCCGTAC951.3656287E-510.99629616
GGCGAGG5100.010.98678119
AATCCCG2500.010.63669519
CTAGTAC1355.685979E-810.55586053
CCGTACA1002.408104E-510.44648217
TAGGACC4400.010.3639354
CCAACGA1409.733594E-810.1754119
CCGTTTC1409.737414E-810.17514413
ACTGTTC5900.010.1406528
GTCCTAG1803.619789E-1010.0738581
GATATAC5500.010.06421851
CGCGCCT856.614882E-410.05543612
GCCCCGT951.6498726E-49.99663314
Produced by FastQC (version 0.11.5)