FastQCFastQC Report
Thu 26 May 2016
SRR1511720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151530
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44920.20878165770405244No Hit
GTCCTACAGTGGACATTTCTAAATT35390.16448759719827286No Hit
CTGTAGGACGTGGAATATGGCAAGA30810.14320042016611434No Hit
GTCCTAAAGTGTGTATTTCTCATTT28480.13237091744014723No Hit
TATCAACGCAGAGTACTTTTTTTTT25970.12070480076968482No Hit
GGTATCAACGCAGAGTACTTTTTTT25810.11996114393013343No Hit
CTTTAGGACGTGAAATATGGCGAGG22670.10536687845393743No Hit
TTGTAGAACAGTGTATATCAATGAG22510.10462322161438604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG557.737206E-616.1766721
GGACCGT457.0027803E-414.7102496
CGCTTAG450.00884703713.1809921
TAGGACC4750.013.1396814
GCGAACC604.2543662E-412.60878511
CCAACGT1750.012.42865919
CGAACCG550.00317618512.03565812
TCCAACG3600.011.82073518
TAGAGCG658.3399104E-411.6388785
GGTATCA11550.011.5547671
GTAGGAC17700.011.3265353
GCGCCAC600.006080659611.03268713
TGTAGGA17450.010.87715152
CGGTAGG650.00792526810.6461861
GTCAACG750.001902511910.5447932
GGCGAGG7050.010.46260819
TTAGGAC10000.010.4022473
GGACGTG16850.010.3264826
GACGTGA8200.010.2638587
GTACTAG1353.7352947E-710.2518831
Produced by FastQC (version 0.11.5)