##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511720_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2151530 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69609208330816 31.0 28.0 34.0 16.0 34.0 2 26.09769838208159 31.0 25.0 34.0 10.0 34.0 3 27.742332665591462 31.0 26.0 34.0 16.0 34.0 4 32.13134792450024 35.0 32.0 37.0 19.0 37.0 5 32.56956863255451 35.0 33.0 37.0 25.0 37.0 6 32.801253991345696 36.0 35.0 37.0 25.0 37.0 7 32.8222186072237 37.0 35.0 37.0 25.0 37.0 8 32.84040473523493 37.0 35.0 37.0 25.0 37.0 9 34.36537022490972 39.0 35.0 39.0 26.0 39.0 10 34.22709374259248 39.0 35.0 39.0 25.0 39.0 11 34.23070837961823 39.0 35.0 39.0 25.0 39.0 12 34.11695955901149 39.0 35.0 39.0 25.0 39.0 13 34.11179486226081 39.0 35.0 39.0 25.0 39.0 14 35.174720780096024 40.0 36.0 41.0 25.0 41.0 15 35.11163776475345 40.0 35.0 41.0 25.0 41.0 16 35.03733064377443 40.0 35.0 41.0 24.0 41.0 17 35.01119157064972 40.0 35.0 41.0 24.0 41.0 18 34.940923435880514 40.0 35.0 41.0 23.0 41.0 19 34.962287767309775 40.0 35.0 41.0 23.0 41.0 20 34.876395867127115 40.0 35.0 41.0 22.0 41.0 21 34.78421123572527 40.0 35.0 41.0 19.0 41.0 22 34.6666409485343 39.0 35.0 41.0 18.0 41.0 23 34.5477464873834 39.0 34.0 41.0 18.0 41.0 24 34.41958978029588 39.0 34.0 41.0 18.0 41.0 25 34.2989189088695 39.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.234484844994693 1101 2 3.6049083407148075 1101 3 2.25177686778029 1101 4 1.3637548920614293 1101 5 0.921800010879096 1101 6 0.7221567822370858 1101 7 0.6320865280310031 1101 8 0.31404683070383044 1101 9 0.31224186019865385 1101 10 0.6406207987551511 1101 11 0.4304277414494706 1101 12 0.5418874270669036 1101 13 0.5240133431206146 1101 14 0.6597849716984925 1101 15 0.4123434233980987 1101 16 0.4894928616510654 1101 17 0.5548193129665577 1101 18 0.5936878936119001 1101 19 0.5389706533436822 1101 20 0.7052586932274636 1101 21 0.7071071254315697 1101 22 0.6072885211571943 1101 23 0.7304465412221504 1101 24 0.680433180059147 1101 25 0.5339074884777162 1102 1 1.7387103568771742 1102 2 4.252518442162831 1102 3 2.89383945523716 1102 4 1.9046615920442633 1102 5 1.4997717185577386 1102 6 1.1614771573857752 1102 7 1.1103700308587605 1102 8 1.2071429728507184 1102 9 1.3761229600360352 1102 10 1.36025767744718 1102 11 1.2888324970830283 1102 12 1.2633058813306022 1102 13 1.338473862924232 1102 14 1.4020097112800016 1102 15 1.42024901931687 1102 16 1.4131399558492532 1102 17 1.6370794665099382 1102 18 1.5832727080304707 1102 19 1.4976719756565728 1102 20 1.5357300842937889 1102 21 1.5249746268670066 1102 22 1.4675074847976646 1102 23 1.3014131528021622 1102 24 1.2418268336720644 1102 25 1.3744195690829457 1103 1 1.7053153885351549 1103 2 4.166118133911034 1103 3 2.7530429896940483 1103 4 1.9518810702421234 1103 5 1.483254610813141 1103 6 1.2268471094363491 1103 7 1.0558856042523956 1103 8 0.9573070386428313 1103 9 1.2279348988062964 1103 10 1.0005847805019883 1103 11 1.2039181835611714 1103 12 1.2815906780540445 1103 13 1.2605758086101986 1103 14 1.396471771209498 1103 15 1.2172672050984517 1103 16 1.0860722087661685 1103 17 1.217587606092124 1103 18 1.2557874087092173 1103 19 1.3474429743509546 1103 20 1.3042088816205322 1103 21 1.3349607263687844 1103 22 1.5134827494329102 1103 23 1.3122672083166478 1103 24 1.5200770441751956 1103 25 1.1629032634377765 1104 1 1.6366543567320022 1104 2 4.1427998896382405 1104 3 2.707331314536539 1104 4 1.8615673477459325 1104 5 1.4496843542744102 1104 6 1.2245999339716036 1104 7 0.9539522921183661 1104 8 0.9914102426777163 1104 9 1.281317114074099 1104 10 1.100573093707105 1104 11 1.2197096871871622 1104 12 1.0337661390956328 1104 13 0.9623090368859195 1104 14 1.2704270940504117 1104 15 1.2792272313183801 1104 16 1.0719561418445664 1104 17 1.148770420919675 1104 18 1.0648081318920433 1104 19 1.1869056980462176 1104 20 1.1174931847725347 1104 21 1.0874509649301132 1104 22 1.376123624109205 1104 23 1.24019526312253 1104 24 1.3384262016264614 1104 25 1.2803244201932742 1105 1 1.5241841423509577 1105 2 3.863876642562129 1105 3 2.4995178965320406 1105 4 1.6669155734763237 1105 5 1.2829459263894805 1105 6 0.966507236388459 1105 7 0.968771396109446 1105 8 0.9070843303263203 1105 9 0.9111338649099565 1105 10 0.6767164596593886 1105 11 0.7311289119821822 1105 12 0.8979036529146711 1105 13 0.90064482235546 1105 14 0.9951623729258188 1105 15 0.9072059994245336 1105 16 0.9291678143428683 1105 17 1.0355060019227764 1105 18 0.9230369519043009 1105 19 0.8333444209180598 1105 20 0.943157258844252 1105 21 1.0030034473615785 1105 22 1.1031595040113302 1105 23 1.0357625172885108 1105 24 1.1023037489434984 1105 25 1.1074972473660978 1106 1 1.5345249872241773 1106 2 3.927413159336055 1106 3 2.4968970162133175 1106 4 1.4716999178290138 1106 5 1.161622006504409 1106 6 1.0358964136913045 1106 7 0.9424577151195024 1106 8 0.8908564867603488 1106 9 0.8806671294072501 1106 10 0.9487995590806122 1106 11 1.1637242942178574 1106 12 1.0377739078928414 1106 13 1.0896905221925266 1106 14 1.1978501727359188 1106 15 1.3901863816854956 1106 16 1.311237915837431 1106 17 1.2618621131709702 1106 18 1.3902340234179178 1106 19 1.3357218729357996 1106 20 1.3428447016971603 1106 21 1.5518516149147814 1106 22 1.3339662671921957 1106 23 1.3555604917627448 1106 24 1.4555186129497315 1106 25 1.4491124666791606 1107 1 1.3811507424682432 1107 2 3.858918384179212 1107 3 2.466753856918686 1107 4 1.6266301359306112 1107 5 1.1858447779184687 1107 6 1.0693738015837937 1107 7 0.9820631525518877 1107 8 0.9455643688063589 1107 9 0.8991352090756806 1107 10 0.8930003026555582 1107 11 0.9146025920312084 1107 12 1.0069820428149825 1107 13 0.9429893373027198 1107 14 0.8381371302841316 1107 15 1.1361474030234717 1107 16 1.081926648608551 1107 17 1.077954294371068 1107 18 0.8635872691669491 1107 19 1.0509987858327037 1107 20 1.0395714990941087 1107 21 1.0904417574026297 1107 22 1.0422519944736806 1107 23 1.2085487619925601 1107 24 1.2760009294899959 1107 25 1.1824394284726623 1108 1 1.4886544486698483 1108 2 4.044421614096418 1108 3 2.57357744899382 1108 4 1.5940791875883846 1108 5 1.205023178239145 1108 6 1.0720054092345208 1108 7 0.8908408286294076 1108 8 0.7762988920629468 1108 9 0.7089159455531586 1108 10 0.5735218613417032 1108 11 0.6218528858459678 1108 12 0.8076274808921013 1108 13 0.792186698105418 1108 14 0.8924619562449081 1108 15 0.8712726049217068 1108 16 0.9497279598449566 1108 17 0.9170326567889546 1108 18 0.9152354949621113 1108 19 0.7992454411618581 1108 20 0.9734375013864565 1108 21 0.7856513270833076 1108 22 0.9255020992289786 1108 23 1.0747253435249107 1108 24 1.0983127630667724 1108 25 1.1407338667615647 1109 1 0.9873025411606768 1109 2 2.971077884811603 1109 3 1.6614449298417107 1109 4 1.1226627559116125 1109 5 0.6049162033436843 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-1.1871476489986534 2314 14 -1.2079489753910124 2314 15 -1.319332049031317 2314 16 -0.9931222750705118 2314 17 -0.9882883670770752 2314 18 -1.0051015762342246 2314 19 -1.0056548345195537 2314 20 -1.0416418081465935 2314 21 -1.0697343678419884 2314 22 -1.1598028000468688 2314 23 -1.0749150344849312 2314 24 -1.0850558219777113 2314 25 -0.9043511781809528 2315 1 -1.9327447008608942 2315 2 -3.8707178334592314 2315 3 -1.8873606302755377 2315 4 -2.0986197277310303 2315 5 -2.019622525173844 2315 6 -1.932932380039727 2315 7 -1.8450931725836632 2315 8 -1.9868642589316856 2315 9 -1.9466670736950817 2315 10 -2.124269543928115 2315 11 -2.06683552886755 2315 12 -2.07323157837034 2315 13 -2.021500595257045 2315 14 -2.222141586199193 2315 15 -2.2811148842057705 2315 16 -2.552227401778403 2315 17 -2.3170144538692625 2315 18 -2.2969761187197264 2315 19 -2.3315136118942448 2315 20 -2.3010950508396704 2315 21 -2.403399489983414 2315 22 -2.333647896532767 2315 23 -2.240685351985533 2315 24 -2.3578343552623977 2315 25 -2.482933212989469 2316 1 -0.7876054325965107 2316 2 1.61570571018067 2316 3 0.26640481078737466 2316 4 -0.7399804544723345 2316 5 -1.0482963206422369 2316 6 -1.2234677009548989 2316 7 -1.6304134346543364 2316 8 -1.5922802427224596 2316 9 -1.7423635149032108 2316 10 -1.8687684647729057 2316 11 -1.6926730170829032 2316 12 -1.8354043646038676 2316 13 -1.7879807820596127 2316 14 -2.110698238197955 2316 15 -2.1071936360277306 2316 16 -2.2459163607413117 2316 17 -2.055392962660399 2316 18 -2.34829288784659 2316 19 -2.156812790291781 2316 20 -1.979386474225798 2316 21 -2.449871163407771 2316 22 -2.157107324676552 2316 23 -2.440529222161125 2316 24 -2.3433102861442165 2316 25 -2.2642249875140266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102721.0 3 2.0 4 839.0 5 4311.0 6 5127.0 7 4587.0 8 4196.0 9 4116.0 10 3853.0 11 3732.0 12 3782.0 13 3630.0 14 3609.0 15 3753.0 16 4075.0 17 4349.0 18 4992.0 19 5996.0 20 7836.0 21 10315.0 22 12357.0 23 14725.0 24 17134.0 25 19474.0 26 21974.0 27 24283.0 28 27997.0 29 33318.0 30 41577.0 31 51581.0 32 65638.0 33 85861.0 34 114682.0 35 160640.0 36 246060.0 37 316660.0 38 711748.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48617030671197 18.492142800704613 12.869911179486227 25.15177571309719 2 16.3340240948635 20.986533794272916 39.948933854522224 22.730508256341363 3 19.359479068383894 23.95885718535182 29.396847824571353 27.284815921692935 4 12.23650146639833 16.693794648459466 38.380826667534265 32.68887721760794 5 13.264514089973181 37.5381705112176 35.94878993088639 13.248525467922828 6 31.91803042486045 37.150446426496494 18.035072715695343 12.896450432947718 7 27.963263352126162 31.372232783182202 21.94884570514936 18.71565815954228 8 24.6433468276064 37.730452282794104 20.57159323830019 17.054607651299307 9 26.006841642923874 15.728946377694013 20.06539532332805 38.19881665605406 10 14.641580642612467 29.498124590407755 34.48606340604128 21.374231360938495 11 34.83688352009965 22.48381384410164 24.161038888604853 18.51826374719386 12 23.041138166792933 24.403610453956023 32.11086993906662 20.44438144018443 13 29.18430140411707 21.732395086287433 25.820369690406363 23.262933819189136 14 20.77921293219244 22.06211393752353 27.673655491673365 29.48501763861066 15 23.021059432125046 30.694621966693468 23.518101072260205 22.766217528921278 16 22.151585151032055 28.284383671154945 27.75169298127379 21.81233819653921 17 21.744247117167784 28.292610374942484 27.928962180401857 22.03418032748788 18 21.411181810153703 27.886108955022703 29.71908362885946 20.98362560596413 19 23.353438415192173 26.87358617987487 27.429841754398847 22.343133650534106 20 23.053547940302945 27.612489716620264 28.274204868163586 21.0597574749132 21 23.039865248942263 26.899875111723183 27.208400746819812 22.851858892514745 22 22.678408503055298 27.933603810163003 27.687683388728562 21.700304298053133 23 22.689768516711005 27.406907501501266 27.564145467660943 22.339178514126782 24 22.854480300065536 26.916984657429825 28.365628180875934 21.8629068616287 25 22.909789777507168 27.20817278866667 28.084432938420566 21.797604495405594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 163.0 2 321.0 3 479.0 4 479.0 5 479.0 6 1174.5 7 1870.0 8 1870.0 9 1870.0 10 2910.0 11 3950.0 12 3950.0 13 3950.0 14 6644.0 15 9338.0 16 9338.0 17 9338.0 18 16718.0 19 24098.0 20 24098.0 21 24098.0 22 39030.5 23 53963.0 24 53963.0 25 53963.0 26 77715.5 27 101468.0 28 101468.0 29 101468.0 30 134327.0 31 167186.0 32 167186.0 33 167186.0 34 186911.0 35 206636.0 36 206636.0 37 206636.0 38 222028.5 39 237421.0 40 237421.0 41 237421.0 42 251359.5 43 265298.0 44 265298.0 45 265298.0 46 270515.0 47 275732.0 48 275732.0 49 275732.0 50 267075.5 51 258419.0 52 258419.0 53 258419.0 54 232286.0 55 206153.0 56 206153.0 57 206153.0 58 177915.0 59 149677.0 60 149677.0 61 149677.0 62 123074.0 63 96471.0 64 96471.0 65 96471.0 66 74641.5 67 52812.0 68 52812.0 69 52812.0 70 38807.0 71 24802.0 72 24802.0 73 24802.0 74 17483.5 75 10165.0 76 10165.0 77 10165.0 78 6949.0 79 3733.0 80 3733.0 81 3733.0 82 2461.5 83 1190.0 84 1190.0 85 1190.0 86 782.5 87 375.0 88 375.0 89 375.0 90 238.0 91 101.0 92 101.0 93 101.0 94 60.5 95 20.0 96 20.0 97 20.0 98 15.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.341793979168313 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.647855247196181E-5 20 0.0 21 3.253498673037327E-4 22 9.760496019111981E-4 23 6.506997346074654E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2151530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.60675395578689 #Duplication Level Percentage of deduplicated Percentage of total 1 84.7269068543273 63.2119949311567 2 10.830486604985612 16.160548987192143 3 2.475879946768257 5.541520980378182 4 0.865732133063629 2.583578569723869 5 0.37222662610946 1.388531015497058 6 0.20279040720144909 0.9077720408803407 7 0.11253168852333528 0.5876936798522805 8 0.08743404059906275 0.5218535963467645 9 0.051569956702254706 0.346272036407613 >10 0.24089496821008222 3.089581251883984 >50 0.01740112225256001 0.9015014611010934 >100 0.012868710052971698 1.8611470883811483 >500 0.0020795973026019893 1.0626501820572993 >1k 0.0011973439014974702 1.8353541791414023 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4492 0.20878165770405244 No Hit GTCCTACAGTGGACATTTCTAAATT 3539 0.16448759719827286 No Hit CTGTAGGACGTGGAATATGGCAAGA 3081 0.14320042016611434 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2848 0.13237091744014723 No Hit TATCAACGCAGAGTACTTTTTTTTT 2597 0.12070480076968482 No Hit GGTATCAACGCAGAGTACTTTTTTT 2581 0.11996114393013343 No Hit CTTTAGGACGTGAAATATGGCGAGG 2267 0.10536687845393743 No Hit TTGTAGAACAGTGTATATCAATGAG 2251 0.10462322161438604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.647855247196181E-5 0.0 10 0.0 0.0 0.0 4.647855247196181E-5 0.0 11 0.0 0.0 0.0 4.647855247196181E-5 0.0 12 0.0 0.0 0.0 4.647855247196181E-5 0.0 13 0.0 0.0 0.0 9.295710494392363E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 55 7.737206E-6 16.176672 1 GGACCGT 45 7.0027803E-4 14.710249 6 CGCTTAG 45 0.008847037 13.180992 1 TAGGACC 475 0.0 13.139681 4 GCGAACC 60 4.2543662E-4 12.608785 11 CCAACGT 175 0.0 12.428659 19 CGAACCG 55 0.003176185 12.035658 12 TCCAACG 360 0.0 11.820735 18 TAGAGCG 65 8.3399104E-4 11.638878 5 GGTATCA 1155 0.0 11.554767 1 GTAGGAC 1770 0.0 11.326535 3 GCGCCAC 60 0.0060806596 11.032687 13 TGTAGGA 1745 0.0 10.8771515 2 CGGTAGG 65 0.007925268 10.646186 1 GTCAACG 75 0.0019025119 10.544793 2 GGCGAGG 705 0.0 10.462608 19 TTAGGAC 1000 0.0 10.402247 3 GGACGTG 1685 0.0 10.326482 6 GACGTGA 820 0.0 10.263858 7 GTACTAG 135 3.7352947E-7 10.251883 1 >>END_MODULE