FastQCFastQC Report
Thu 26 May 2016
SRR1511720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151530
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56640.2632545212011917No Hit
GTCCTACAGTGGACATTTCTAAATT41690.1937690852556088No Hit
CTGTAGGACGTGGAATATGGCAAGA36020.16741574600400647No Hit
GGTATCAACGCAGAGTACTTTTTTT33340.1549594939415207No Hit
TATCAACGCAGAGTACTTTTTTTTT32820.15254260921297869No Hit
GTCCTAAAGTGTGTATTTCTCATTT31080.14445534108285732No Hit
CTTTAGGACGTGAAATATGGCGAGG26490.12312168549822684No Hit
TTGTAGAACAGTGTATATCAATGAG25300.11759073775406338No Hit
GATATACACTGTTCTACAAATCCCG22690.10545983555888135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGT350.002174896716.2808536
TCCAACG4500.012.66288718
TAGGACC6600.012.0909544
TACACCG550.003069019412.0909545
AAGCGAC658.045739E-411.68881914
TGGTCGG658.045739E-411.68881910
CCAACGT2200.011.65588219
GAACAGT7200.011.2119316
TGTAGAA12450.010.9879922
GGTATCA12800.010.8810021
ACGACTG700.001491760410.85718255
CCCCGTA700.001495525410.85390215
CCAACGA2950.010.62419419
GACGTGA8850.010.6239477
TGTAGGA19050.010.5722172
TGCTCGA909.5469004E-510.55240510
AATCCCG5050.010.531754519
CGTTTCC2450.010.46626314
TTAGGAC11800.010.3856323
GTAGAAC12300.010.3496323
Produced by FastQC (version 0.11.5)