##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511720_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2151530 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64247907303175 33.0 31.0 34.0 30.0 34.0 2 31.811708412153212 34.0 31.0 34.0 30.0 34.0 3 31.93057266224501 34.0 31.0 34.0 30.0 34.0 4 35.45424047073478 37.0 35.0 37.0 33.0 37.0 5 35.19405353399674 37.0 35.0 37.0 32.0 37.0 6 35.17490111687962 37.0 35.0 37.0 32.0 37.0 7 35.03956858607596 37.0 35.0 37.0 32.0 37.0 8 34.9714919150558 37.0 35.0 37.0 32.0 37.0 9 36.5834671141002 39.0 37.0 39.0 32.0 39.0 10 36.42956500722742 39.0 37.0 39.0 32.0 39.0 11 36.43607897635636 39.0 37.0 39.0 32.0 39.0 12 36.305133091335 39.0 37.0 39.0 32.0 39.0 13 36.30561832742281 39.0 37.0 39.0 32.0 39.0 14 37.478085827295 40.0 38.0 41.0 32.0 41.0 15 37.424363127634756 40.0 37.0 41.0 32.0 41.0 16 37.28523887652043 40.0 37.0 41.0 32.0 41.0 17 37.275176734695776 40.0 37.0 41.0 32.0 41.0 18 37.2184431544064 40.0 37.0 41.0 32.0 41.0 19 37.240823042207175 40.0 37.0 41.0 31.0 41.0 20 37.33469670420585 40.0 38.0 41.0 32.0 41.0 21 37.29091809084698 40.0 38.0 41.0 32.0 41.0 22 37.210811376090504 40.0 37.0 41.0 32.0 41.0 23 37.13146412088142 40.0 37.0 41.0 31.0 41.0 24 37.017746440904844 40.0 37.0 41.0 31.0 41.0 25 36.56277486253968 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.021288961315150345 1101 2 0.0487005049818805 1101 3 0.07545825394106842 1101 4 -0.008066254381667193 1101 5 0.09655258827685742 1101 6 -0.134672334263513 1101 7 -0.0024615844214608273 1101 8 -0.08791559613129607 1101 9 -0.06933391514476739 1101 10 -0.0763263869889741 1101 11 -0.10818184540806897 1101 12 0.060620758893122684 1101 13 0.03134503607049055 1101 14 -0.07319402560805344 1101 15 -0.0807172622549075 1101 16 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1103 3 0.3139648865046887 1103 4 0.37493736211661144 1103 5 0.41435806963099964 1103 6 0.44837700008611137 1103 7 0.5075279977776148 1103 8 0.4587664230841 1103 9 0.5366399870669127 1103 10 0.5855213710214926 1103 11 0.41233210973437906 1103 12 0.6229758151561953 1103 13 0.5410094963874741 1103 14 0.8204131205863661 1103 15 0.7113970261953853 1103 16 0.7779785421829786 1103 17 0.7866681412077483 1103 18 0.6946254389278366 1103 19 0.7402807708000267 1103 20 0.7327807459169335 1103 21 0.7915065277817206 1103 22 0.6483196322349656 1103 23 0.7616352375402329 1103 24 0.7019174603207929 1103 25 0.8627367200360894 1104 1 0.1984178704809132 1104 2 0.2771610155114317 1104 3 0.27900654511043044 1104 4 0.33377365383976354 1104 5 0.31917789656266393 1104 6 0.29982301240592335 1104 7 0.3479664329528376 1104 8 0.45107216347467727 1104 9 0.505567937358748 1104 10 0.5260012411967665 1104 11 0.5174243155810885 1104 12 0.6575566211892507 1104 13 0.5115528434015175 1104 14 0.6791308889372871 1104 15 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-0.656650456657637 2314 14 -0.684777674360511 2314 15 -0.8288169057289068 2314 16 -0.5704851605426313 2314 17 -0.758882739911904 2314 18 -0.7240787521759984 2314 19 -0.7390129913445449 2314 20 -0.5883458951110612 2314 21 -0.643454042150772 2314 22 -0.5516659595811859 2314 23 -0.530153380586647 2314 24 -0.5922660742830246 2314 25 -0.8344145056957402 2315 1 -0.5335720013174985 2315 2 -0.4722220011916818 2315 3 -0.5406549198866912 2315 4 -0.5055240927686384 2315 5 -0.6120208615719633 2315 6 -0.8318213989585033 2315 7 -0.7027834969913869 2315 8 -0.8179655781936077 2315 9 -0.7455668117160386 2315 10 -0.884027540091509 2315 11 -0.9167884342032409 2315 12 -0.905173087886638 2315 13 -0.8621618431280922 2315 14 -1.0985639312998785 2315 15 -1.1159819704038085 2315 16 -1.1220216764670283 2315 17 -1.1628406019104887 2315 18 -1.2882236542013672 2315 19 -1.0437325740064693 2315 20 -1.1049875053461236 2315 21 -0.9762617175181489 2315 22 -1.0795109959007974 2315 23 -0.9250673235073492 2315 24 -1.0113967948052078 2315 25 -0.9320739598640699 2316 1 -0.655580236551053 2316 2 -0.7940871686432232 2316 3 -0.6973659283455156 2316 4 -0.687392441211685 2316 5 -0.7856077214646575 2316 6 -0.8811762493174413 2316 7 -1.0148160199402412 2316 8 -1.5030116142095054 2316 9 -1.1836349817469554 2316 10 -1.1544107419976442 2316 11 -1.0983283875333285 2316 12 -1.2878044132767101 2316 13 -1.4348219126949928 2316 14 -1.2869055206123647 2316 15 -1.2952371420121551 2316 16 -1.2163486777832233 2316 17 -1.1946113307955102 2316 18 -1.2868174100840903 2316 19 -1.2900908381686023 2316 20 -1.283446660723726 2316 21 -1.3424938791862786 2316 22 -1.4924623951144653 2316 23 -1.5737105230623172 2316 24 -1.5914138676629719 2316 25 -1.7217088450448728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16518.0 3 0.0 4 311.0 5 2650.0 6 3569.0 7 2966.0 8 2542.0 9 2243.0 10 2172.0 11 2055.0 12 2055.0 13 1990.0 14 2044.0 15 2112.0 16 2042.0 17 2183.0 18 2407.0 19 2697.0 20 3385.0 21 4345.0 22 5692.0 23 7445.0 24 9121.0 25 11018.0 26 13453.0 27 15266.0 28 17993.0 29 22759.0 30 29096.0 31 39400.0 32 51211.0 33 68216.0 34 91653.0 35 124736.0 36 187259.0 37 340455.0 38 1056471.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64903543815306 18.9700820324591 13.343825296212763 25.03705723317508 2 14.657104479138102 20.274781202214236 41.4591011977523 23.609013120895366 3 19.293572480978654 24.144213652610002 29.722197691875085 26.840016174536256 4 12.4817223092404 16.95686325545077 38.255427532964916 32.30598690234391 5 13.169958982055697 37.622363381232546 36.00089456500614 13.206783071705615 6 31.83660000092957 37.41379390480263 17.935376220642986 12.814229873624816 7 27.904560940354074 31.338582311192496 22.00782698823628 18.749029760217148 8 24.63767644420482 37.91222990151194 20.4494011238514 17.00069253043183 9 25.953298350476175 15.71179579183186 20.06372209543906 38.27118376225291 10 14.583064145050267 29.679809252020657 34.47035365530576 21.26677294762332 11 34.92077730731154 22.397038386636485 24.185440128652633 18.49674417739934 12 22.96551756192105 24.417507541145138 32.25764920777308 20.359325689160737 13 29.16078325656626 21.67164761820658 25.921971806110072 23.245597319117092 14 20.6138422424972 22.049425292698686 27.786040631550573 29.550691833253545 15 22.983458283175228 30.77047496432771 23.563045832500592 22.68302091999647 16 22.25100277476958 28.282152700636292 27.727849483855678 21.738995040738452 17 21.641250644889915 28.318452450116894 28.006209534610253 22.034087370382938 18 21.35522163297746 27.90404967627688 29.730610309872507 21.01011838087315 19 23.33860090261349 26.86590472826314 27.46101611411414 22.334478255009227 20 22.959242957337338 27.574470260698202 28.36781267284212 21.098474109122346 21 22.991452594200407 26.847406264379302 27.33650007204176 22.824641069378533 22 22.62101853099887 27.855572546048624 27.839583923998273 21.683824998954233 23 22.703517961636603 27.332456437976692 27.635496600093884 22.328529000292814 24 22.818738293214597 26.890120054101036 28.492375193466973 21.798766459217394 25 22.873321685586124 27.176852867944014 28.151433727990245 21.79839171847962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 135.0 2 281.0 3 427.0 4 427.0 5 427.0 6 1192.0 7 1957.0 8 1957.0 9 1957.0 10 2871.0 11 3785.0 12 3785.0 13 3785.0 14 6387.0 15 8989.0 16 8989.0 17 8989.0 18 16417.5 19 23846.0 20 23846.0 21 23846.0 22 39095.0 23 54344.0 24 54344.0 25 54344.0 26 78684.5 27 103025.0 28 103025.0 29 103025.0 30 136521.5 31 170018.0 32 170018.0 33 170018.0 34 188895.0 35 207772.0 36 207772.0 37 207772.0 38 222870.5 39 237969.0 40 237969.0 41 237969.0 42 251664.0 43 265359.0 44 265359.0 45 265359.0 46 270915.5 47 276472.0 48 276472.0 49 276472.0 50 267262.5 51 258053.0 52 258053.0 53 258053.0 54 231849.0 55 205645.0 56 205645.0 57 205645.0 58 177399.0 59 149153.0 60 149153.0 61 149153.0 62 122008.5 63 94864.0 64 94864.0 65 94864.0 66 73111.0 67 51358.0 68 51358.0 69 51358.0 70 37661.5 71 23965.0 72 23965.0 73 23965.0 74 16734.5 75 9504.0 76 9504.0 77 9504.0 78 6500.5 79 3497.0 80 3497.0 81 3497.0 82 2262.0 83 1027.0 84 1027.0 85 1027.0 86 662.0 87 297.0 88 297.0 89 297.0 90 181.0 91 65.0 92 65.0 93 65.0 94 34.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44493918281409045 2 0.0 3 0.0 4 0.0 5 0.03550961408857882 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0019056206513504345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2151530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.24665562362648 #Duplication Level Percentage of deduplicated Percentage of total 1 82.55488019129062 58.81759119038625 2 12.076730077510941 17.20853257783828 3 2.9584744889485206 6.323442392561987 4 1.0423239229908314 2.970483743583805 5 0.4864359419080413 1.7328467018038296 6 0.22980679164984102 0.9823779206788021 7 0.1372917337340701 0.6847103811325341 8 0.10214231554960358 0.5821838704449915 9 0.07340937359017789 0.47071551237529907 >10 0.3000294738831196 3.6177356152658184 >50 0.01969001266513866 0.9723916529429933 >100 0.01507043815815862 2.1568560510344192 >500 0.002085747716988831 1.0412859259935567 >1k 0.0015643107877411128 2.175819951678647 >5k 6.51796161558797E-5 0.2630265122787352 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5664 0.2632545212011917 No Hit GTCCTACAGTGGACATTTCTAAATT 4169 0.1937690852556088 No Hit CTGTAGGACGTGGAATATGGCAAGA 3602 0.16741574600400647 No Hit GGTATCAACGCAGAGTACTTTTTTT 3334 0.1549594939415207 No Hit TATCAACGCAGAGTACTTTTTTTTT 3282 0.15254260921297869 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3108 0.14445534108285732 No Hit CTTTAGGACGTGAAATATGGCGAGG 2649 0.12312168549822684 No Hit TTGTAGAACAGTGTATATCAATGAG 2530 0.11759073775406338 No Hit GATATACACTGTTCTACAAATCCCG 2269 0.10545983555888135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 9.295710494392363E-5 0.0 11 0.0 0.0 0.0 9.295710494392363E-5 0.0 12 0.0 0.0 0.0 9.295710494392363E-5 0.0 13 0.0 0.0 0.0 9.295710494392363E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATCGT 35 0.0021748967 16.280853 6 TCCAACG 450 0.0 12.662887 18 TAGGACC 660 0.0 12.090954 4 TACACCG 55 0.0030690194 12.090954 5 AAGCGAC 65 8.045739E-4 11.688819 14 TGGTCGG 65 8.045739E-4 11.688819 10 CCAACGT 220 0.0 11.655882 19 GAACAGT 720 0.0 11.211931 6 TGTAGAA 1245 0.0 10.987992 2 GGTATCA 1280 0.0 10.881002 1 ACGACTG 70 0.0014917604 10.8571825 5 CCCCGTA 70 0.0014955254 10.853902 15 CCAACGA 295 0.0 10.624194 19 GACGTGA 885 0.0 10.623947 7 TGTAGGA 1905 0.0 10.572217 2 TGCTCGA 90 9.5469004E-5 10.552405 10 AATCCCG 505 0.0 10.5317545 19 CGTTTCC 245 0.0 10.466263 14 TTAGGAC 1180 0.0 10.385632 3 GTAGAAC 1230 0.0 10.349632 3 >>END_MODULE