FastQCFastQC Report
Thu 26 May 2016
SRR1511719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214736
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51800.23388792163038843No Hit
GTATCAACGCAGAGTACTTTTTTTT51660.23325579211246847No Hit
CTGTAGGACGTGGAATATGGCAAGA44350.20024960085536156No Hit
GTCCTAAAGTGTGTATTTCTCATTT37530.16945586291097448No Hit
CTTTAGGACGTGAAATATGGCGAGG32720.14773769875958126No Hit
GGTATCAACGCAGAGTACTTTTTTT28910.13053474545047355No Hit
TATCAACGCAGAGTACTTTTTTTTT28870.13035413701678214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1054.7293724E-1115.0664431
TATACCG457.004434E-414.7098235
TAGGCCG400.005443956714.1844715
TAGGACC6550.013.8596374
GTCTACG450.00883763813.183141
ACCGTCC655.6955214E-513.0933598
GCCGGTT1700.012.23758411
GCACCGT1354.0199666E-1011.9079516
GGTATCA9950.011.5269661
GATATAC7750.011.3545111
TAGACAG3850.011.2984545
ACTGTTC8550.011.2812188
TCCAACG2900.011.08721418
CCGGTTT1900.010.94941712
CGGTTTC2100.010.80721613
GGCGAGG10950.010.79536719
AGGACGT25650.010.7282165
TGTAGGA25050.010.6570292
GTAGGAC26100.010.6519413
GGACGTG25500.010.5688226
Produced by FastQC (version 0.11.5)