FastQCFastQC Report
Thu 26 May 2016
SRR1511719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214736
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59450.2684292845738725No Hit
GTCCTACAGTGGACATTTCTAAATT57510.2596697755398386No Hit
CTGTAGGACGTGGAATATGGCAAGA48770.22020683277826342No Hit
GTCCTAAAGTGTGTATTTCTCATTT43110.19465073941092753No Hit
CTTTAGGACGTGAAATATGGCGAGG37620.16986223188678018No Hit
TATCAACGCAGAGTACTTTTTTTTT34340.15505234032408377No Hit
GGTATCAACGCAGAGTACTTTTTTT34240.15460081923985522No Hit
GTCCTACAGTGTGCATTTCTCATTT24420.1102614487686117No Hit
CTGAAGGACCTGGAATATGGCGAGA22660.10231467768618922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAT551.965454E-413.81427419
TAGGACC7500.013.6816924
GGACCGT500.001503610513.2956376
GGTATCA11300.012.4087031
CGCCCTA1104.992344E-711.2235916
AGGACGT24750.010.9023575
GGCGAGG9900.010.84036719
TTAGGAC14200.010.8393683
GGACGTG24400.010.7423776
TCGAACT1158.8532397E-710.736093519
GACGTGA12400.010.7222897
GTCCTAT2250.010.5985141
GTATCAA24350.010.49840451
ACGTGAA14100.010.4398378
TAGGACG26850.010.4035214
GTCCTAC27150.010.3642921
GTAGGAC26550.010.3421453
AGGACCT17550.010.3403095
TGTAGGA26750.010.3003392
CTAATAC1201.5219084E-610.2929393
Produced by FastQC (version 0.11.5)