FastQCFastQC Report
Thu 26 May 2016
SRR1511718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46360.2362656571864817No Hit
TATCAACGCAGAGTACTTTTTTTTT28540.14544913408330862No Hit
GGTATCAACGCAGAGTACTTTTTTT28140.1434106038228558No Hit
GTCCTACAGTGGACATTTCTAAATT22380.1140557680723352No Hit
GTCCTAAAGTGTGTATTTCTCATTT21010.1070738019302843No Hit
CTGTAGGACGTGGAATATGGCAAGA20730.10564683074796732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC500.001551816413.2399418
GCGTTAA550.002282198812.5779931
TAGGACC3950.012.2101214
CGAGACA550.003175335212.0360014
GGTATCA11650.011.5368961
GCGCCAC1105.271231E-711.17628713
AGGACCG855.5768654E-511.1257155
CGTGCGC700.001549419410.80783810
CGGTTTC909.966405E-510.50762113
TTAGACA2700.010.507624
TGGCGAG11600.010.4354218
GGCGAGG5900.010.418838519
GGCGTGC1002.522807E-510.4025448
TCCAACG1752.564775E-1010.26770818
TTAGGAC7900.010.0553943
GCACCGT856.8763085E-410.0131446
GACGTGA7300.09.9750427
GCCGGTT951.7215812E-49.95458811
GTATCAA22300.09.9270271
GTCCTAC10400.09.88270951
Produced by FastQC (version 0.11.5)