FastQCFastQC Report
Thu 26 May 2016
SRR1511718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57270.29186657004033234No Hit
TATCAACGCAGAGTACTTTTTTTTT37790.19259014635628005No Hit
GGTATCAACGCAGAGTACTTTTTTT36110.1840283192623782No Hit
GTCCTACAGTGGACATTTCTAAATT25890.1319438711078087No Hit
CTGTAGGACGTGGAATATGGCAAGA23630.12042617513625027No Hit
GTCCTAAAGTGTGTATTTCTCATTT23010.11726645323254839No Hit
CTTTAGGACGTGAAATATGGCGAGG20730.10564683074796732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG658.0267835E-411.692015
TATGTCG855.3408912E-511.17305316
GGCGAGG5200.011.14110819
GACGTGG7450.010.9630897
GTCCTAG3150.010.9046751
GGTATCA11950.010.6993561
GGACCGC803.7822998E-410.6842326
TAGGACT2700.010.5552864
GGACGTG14800.010.4596396
TAGGACG15150.010.3462714
AGGACGT15050.010.28877455
GCGACTA1201.5299829E-610.28851916
TCTATAC2053.6379788E-1210.1948623
TAGGACC3850.010.1166264
GTCTAGG1701.4460966E-910.10285951
GGACTGT2450.010.078556
GACAGCG856.613155E-410.0557487
GACGTGA7100.010.0321437
TTAGGAC8600.09.9416073
TGTAGGA14150.09.9361422
Produced by FastQC (version 0.11.5)