FastQCFastQC Report
Thu 26 May 2016
SRR1511717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248707
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46770.20798618939684002No Hit
TATCAACGCAGAGTACTTTTTTTTT27680.12309295964303042No Hit
GGTATCAACGCAGAGTACTTTTTTT27360.12166991964715725No Hit
GTCCTACAGTGGACATTTCTAAATT26090.11602222966353554No Hit
CTGTAGGACGTGGAATATGGCAAGA25180.11197545967527116No Hit
GTCCTAAAGTGTGTATTTCTCATTT22710.10099136970712502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3500.011.3475964
GCACCGT1201.3599856E-711.0323856
GTCCTAT1957.2759576E-1210.6487681
GTATCAA22050.010.4486951
ACTGTTC6700.010.1620268
GTATTGG2450.010.0899411
AAGGCGT1502.6271664E-810.0867526
GTCTAGA2850.010.0616181
AATCCCG3300.010.02944119
GGTATCA11150.010.0211661
ATAGGAC2650.09.9915933
CTAGGAC2850.09.9540313
GCGTGCG1054.3195985E-59.9066319
GAACAGT5000.09.8345846
GATATAC6350.09.8102831
GTCCTAA8750.09.7186311
TATACTG3900.09.69885
TCCAACG2054.5474735E-119.68697318
GTCTTAA2154.7293724E-119.6581861
TGTAGGA14100.09.6076282
Produced by FastQC (version 0.11.5)