FastQCFastQC Report
Thu 26 May 2016
SRR1511717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248707
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53470.23778108931043485No Hit
TATCAACGCAGAGTACTTTTTTTTT30960.13767911960073054No Hit
GTCCTACAGTGGACATTTCTAAATT30840.1371454796022781No Hit
GGTATCAACGCAGAGTACTTTTTTT30810.137012069602665No Hit
CTGTAGGACGTGGAATATGGCAAGA28920.12860723962703902No Hit
GTCCTAAAGTGTGTATTTCTCATTT26310.11700056966069834No Hit
CTTTAGGACGTGAAATATGGCGAGG22850.10161394970531953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT550.00306810312.09147554
CGCCCTA1157.109156E-811.56164516
TAGGACC4050.011.4943664
TCCAACG2450.011.24142418
GGTATCA12500.011.2184371
CCAACGA1451.2241799E-911.1344919
CCGTGCG600.00589057411.0799099
GGCGAGG6950.010.52192819
GTATCAA25950.010.2930881
CTAGGGT1201.5234491E-610.2921494
TGGCGAG15000.010.13020318
GTACTAG1909.276846E-1110.0415661
ATAGGAC1901.0004442E-1010.0004683
ACTGTTC6850.09.9823168
CGAAATC1054.1203915E-59.94930613
GTCCTAA7800.09.906391
AGGACGT14700.09.8882185
GTATAAG2550.09.7265361
AGGACCT11600.09.6642465
TATGGCG15550.09.64975116
Produced by FastQC (version 0.11.5)