FastQCFastQC Report
Thu 26 May 2016
SRR1511716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615133
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46980.17964669483349413No Hit
TATCAACGCAGAGTACTTTTTTTTT28700.10974585231420353No Hit
GGTATCAACGCAGAGTACTTTTTTT28010.10710736318191082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.012.0724244
GGTATCA11950.011.5800621
TAGACCG600.006080564611.0328555
TTAGGAC4900.011.0006883
CGTGCGC1159.3574454E-710.69021910
GTATCAA22550.010.3008261
CTAGGAC3150.09.9070533
TAGGACG7950.09.8730664
GTCCTAC10350.09.7411581
CTTAGAC2253.6379788E-129.6668823
AGGACGT8000.09.5749425
GTATTAG2403.6379788E-129.472561
GCCTTAC2301.8189894E-119.4546531
GACGTGA3850.09.3339177
TCCTACA11350.09.2312562
TCCAACG1357.2587445E-69.106482518
GTATAGG3150.09.0999341
GTAGGAC10950.08.8953733
GTCCTAT2251.1623342E-98.7861421
CCGCTCA1401.1499926E-58.7812529
Produced by FastQC (version 0.11.5)