FastQCFastQC Report
Thu 26 May 2016
SRR1511716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615133
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60180.2301221391034414No Hit
GGTATCAACGCAGAGTACTTTTTTT36960.14133124395585234No Hit
TATCAACGCAGAGTACTTTTTTTTT36310.13884571071528676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG1103.8182407E-812.0907994
GGTATCA13200.011.7080231
ACCGTCC1354.7548383E-911.2559278
GTATCAA28150.010.4040511
TTAGGAC5700.010.333243
TAGGACT3300.010.0756664
GTAGGAC10950.09.9770793
TAGGACC5050.09.9702064
ATTACAC2900.09.82749753
TGGCGAG9000.09.81376318
TAGGACG8750.09.7713394
CCTAGAC2500.09.4999143
CTAGTAC1401.0593176E-69.4999143
TACACCG900.00111414059.4999145
GGACGTG9200.09.49718956
AGGACGT9350.09.4491125
GTCCTAA5750.09.2910051
TGTAGGA12500.09.2719152
GTATTAA3400.09.2592861
CGCCCTA1451.7140392E-69.169716
Produced by FastQC (version 0.11.5)