FastQCFastQC Report
Thu 26 May 2016
SRR1511715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58850.3266185517117365No Hit
GGTATCAACGCAGAGTACTTTTTTT34180.18969961083274686No Hit
TATCAACGCAGAGTACTTTTTTTTT32650.18120808349002884No Hit
GTCCTACAGTGGACATTTCTAAATT27070.15023898376952774No Hit
CTGTAGGACGTGGAATATGGCAAGA26400.14652047179591918No Hit
GTCCTAAAGTGTGTATTTCTCATTT21210.11771587904513053No Hit
CTTTAGGACGTGAAATATGGCGAGG19630.1089468508088596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.012.9178291
TCCAACG1403.6379788E-1212.833652518
AACCGTG550.003176374612.0353887
CGTCAGA550.003176374612.03538814
CCAACGA951.0995027E-611.94489719
GACCTAC1251.0137228E-811.8652641
ATAGGAC2450.011.5792353
GCGACTA600.006081024711.03243916
AACACCG600.006081024711.0324395
CTAGACT2750.011.0037824
TAGGACC5850.010.9920274
TAGGACA5200.010.7293494
GGCGAGG5850.010.34543719
AATCCCG2600.010.18378919
TAGCACC1252.7255064E-69.834634
GTAGAAC6400.09.7518873
GTAGGAC15500.09.7004113
CCAGTAC2550.09.6417943
AGGACGT15100.09.5816265
GTATCAA24000.09.4757321
Produced by FastQC (version 0.11.5)