##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511715_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1801796 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.745617150887227 31.0 28.0 34.0 16.0 34.0 2 26.132553296821616 31.0 25.0 34.0 10.0 34.0 3 27.792982113402406 31.0 26.0 34.0 16.0 34.0 4 32.20498824506215 35.0 32.0 37.0 19.0 37.0 5 32.64180739661982 35.0 33.0 37.0 25.0 37.0 6 32.88153986355836 36.0 35.0 37.0 26.0 37.0 7 32.92045769887379 37.0 35.0 37.0 26.0 37.0 8 32.93103658793781 37.0 35.0 37.0 26.0 37.0 9 34.457238777308866 39.0 35.0 39.0 27.0 39.0 10 34.33248325559608 39.0 35.0 39.0 26.0 39.0 11 34.331386017062975 39.0 35.0 39.0 26.0 39.0 12 34.23848759793007 39.0 35.0 39.0 25.0 39.0 13 34.22074141578736 39.0 35.0 39.0 25.0 39.0 14 35.295160495416795 40.0 36.0 41.0 25.0 41.0 15 35.22060655035309 40.0 36.0 41.0 25.0 41.0 16 35.15447031739442 40.0 36.0 41.0 25.0 41.0 17 35.13521064537828 40.0 36.0 41.0 25.0 41.0 18 35.07060066733415 40.0 35.0 41.0 24.0 41.0 19 35.106268967186075 40.0 35.0 41.0 24.0 41.0 20 35.02607065394751 40.0 35.0 41.0 24.0 41.0 21 34.935797393267606 40.0 35.0 41.0 22.0 41.0 22 34.81746435223521 40.0 35.0 41.0 21.0 41.0 23 34.70084848673213 39.0 35.0 41.0 19.0 41.0 24 34.57275129925918 39.0 35.0 41.0 18.0 41.0 25 34.45805240992876 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.654027054871026 1101 2 4.26344153103549 1101 3 2.7462036522925146 1101 4 1.9139494144251472 1101 5 1.4914538046250598 1101 6 1.1812147334083178 1101 7 1.163146036522697 1101 8 0.8869337579524839 1101 9 0.9623879354004714 1101 10 1.3016692261312457 1101 11 1.0326233325025598 1101 12 1.25558667678537 1101 13 1.2531161799513697 1101 14 1.4002176695730313 1101 15 1.346875930867597 1101 16 1.5670238651886805 1101 17 1.5691104618273641 1101 18 1.5887334840223488 1101 19 1.6472960584822687 1101 20 1.5852980149353684 1101 21 1.6168261575802987 1101 22 1.5434684151423994 1101 23 1.6789375127616495 1101 24 1.724693019047038 1101 25 1.513957146445506 1102 1 1.8542329264139106 1102 2 4.360537469096261 1102 3 3.014726285750683 1102 4 2.1493544352565337 1102 5 1.6667837315891063 1102 6 1.3961607191534569 1102 7 1.2419049137821006 1102 8 1.265140041078304 1102 9 1.3942265180177387 1102 10 1.3152274406940805 1102 11 1.483982877423152 1102 12 1.3429271383508095 1102 13 1.6633882971291882 1102 14 1.6379176256121255 1102 15 1.5993244089315937 1102 16 1.7242033049394223 1102 17 1.693945408261527 1102 18 1.5682143930601171 1102 19 1.7165669080807362 1102 20 1.6859800895375798 1102 21 1.5386001761271544 1102 22 1.4938658649296812 1102 23 1.4719973756580842 1102 24 1.3987705808105062 1102 25 1.410324326469798 1103 1 1.453404583292702 1103 2 4.085311898671694 1103 3 2.710443624732104 1103 4 1.6101777721199184 1103 5 1.132297546935213 1103 6 1.0282650803742115 1103 7 0.781820577592633 1103 8 0.6981366714816915 1103 9 0.9423763206556472 1103 10 0.6625007631163342 1103 11 0.8451578076243607 1103 12 0.9719179923028634 1103 13 1.0821117013845054 1103 14 1.146281156949378 1103 15 0.9397653447167045 1103 16 0.8545088792149613 1103 17 0.9381679941862373 1103 18 1.0150552518258849 1103 19 0.9873238157537898 1103 20 0.9617582738063746 1103 21 0.983888709871259 1103 22 1.200340111584154 1103 23 1.0830377145432308 1103 24 1.3417639378914714 1103 25 0.8884885702360421 1104 1 1.710949165292977 1104 2 4.241350661596531 1104 3 2.8286030349421445 1104 4 1.9403510924704506 1104 5 1.5305137105184698 1104 6 1.3482591453809363 1104 7 1.2791236795539618 1104 8 1.2278081139632633 1104 9 1.471188301923327 1104 10 1.3334773477131705 1104 11 1.4938162209410066 1104 12 1.418468370195015 1104 13 1.229212364434538 1104 14 1.4472524556928263 1104 15 1.5780757263182252 1104 16 1.2735869151031238 1104 17 1.5239314290636017 1104 18 1.3523123575893194 1104 19 1.3664849415478244 1104 20 1.604215361022959 1104 21 1.6796093942501713 1104 22 1.6686311117232506 1104 23 1.6032762270855443 1104 24 1.6483456599668642 1104 25 1.4459109284685283 1105 1 1.706421377325558 1105 2 4.0016891466468 1105 3 2.6046501867601997 1105 4 1.7320663915314398 1105 5 1.382741513438667 1105 6 1.2169260422558779 1105 7 1.0887360583046615 1105 8 1.1653409912547232 1105 9 1.0642356534703694 1105 10 0.9555757642603453 1105 11 1.1204309508998875 1105 12 1.1489540056648764 1105 13 1.173937995257205 1105 14 1.0728556318498477 1105 15 1.2333158869763494 1105 16 1.1413483978165289 1105 17 1.3118210395347063 1105 18 1.095758862314149 1105 19 1.3019402070959387 1105 20 1.4044365330842794 1105 21 1.3408407073086792 1105 22 1.235790072687891 1105 23 1.284742560559799 1105 24 1.3124513492308765 1105 25 1.3818007928141824 1106 1 0.9742960012224522 1106 2 3.4528140454825156 1106 3 1.9580868920229832 1106 4 1.2691330253401318 1106 5 0.6637187874297581 1106 6 0.60296975162764 1106 7 0.4802349379464701 1106 8 0.33807418118114185 1106 9 0.17024782238861746 1106 10 0.19015768722786675 1106 11 0.27830084212853023 1106 12 0.34000738240167294 1106 13 0.36773375921907103 1106 14 0.414547878795112 1106 15 0.6080660016925492 1106 16 0.407486376499115 1106 17 0.29815350967948717 1106 18 0.42016786886587454 1106 19 0.48924425442742603 1106 20 0.4727869841679748 1106 21 0.6061505690611568 1106 22 0.3998836426999759 1106 23 0.506384256367248 1106 24 0.25743638111737965 1106 25 0.4096051187850094 1107 1 1.5915397361107217 1107 2 3.9443862046815994 1107 3 2.720049008412822 1107 4 1.8587109060576665 1107 5 1.4501885647873536 1107 6 1.3832481629052893 1107 7 1.1577147733118593 1107 8 1.1716414850156696 1107 9 1.258334447583401 1107 10 1.1616636755380583 1107 11 1.3402369739015825 1107 12 1.2070936161929495 1107 13 1.0904713611657968 1107 14 1.1346404428639474 1107 15 1.2901887487731614 1107 16 1.1826173005627538 1107 17 1.3570875193755114 1107 18 1.2728763566020334 1107 19 1.3209060196980857 1107 20 1.411593211651038 1107 21 1.479568306095203 1107 22 1.5240623286137804 1107 23 1.3934263489950496 1107 24 1.6838946043088896 1107 25 1.5749841305687866 1108 1 1.5617664822632946 1108 2 3.997637486127715 1108 3 2.5135768393435782 1108 4 1.7431800741657 1108 5 1.286805300051661 1108 6 1.0441590993986622 1108 7 0.9121851695224379 1108 8 1.015734551862181 1108 9 0.9152674295467591 1108 10 0.764967340017698 1108 11 0.9488238806817435 1108 12 0.9747537652255929 1108 13 0.9809033445239734 1108 14 1.0211850356793377 1108 15 1.0729521579035222 1108 16 1.1462112637869097 1108 17 1.0194797259327544 1108 18 1.1877458655599753 1108 19 0.9809819836728266 1108 20 1.2379415687722783 1108 21 1.1195596476291527 1108 22 1.1518237583721458 1108 23 1.0278188464547995 1108 24 1.023339405901723 1108 25 1.1342702668003497 1109 1 1.3485556490943686 1109 2 3.2647386406044596 1109 3 2.2790010432172316 1109 4 1.4953859615208742 1109 5 1.124916638686834 1109 6 0.8701712224249292 1109 7 0.8511652570095976 1109 8 0.7833391316721077 1109 9 0.8746634552078447 1109 10 0.9129063575219334 1109 11 0.6746305517477111 1109 12 0.696424184905446 1109 13 0.7855947243856036 1109 14 0.8492414737410172 1109 15 0.959276676758158 1109 16 1.0286172286968025 1109 17 0.9498471583955279 1109 18 0.7952952046441482 1109 19 1.0113507767446066 1109 20 1.076427074884819 1109 21 0.9731343076469727 1109 22 1.100569617018479 1109 23 0.8816044008689516 1109 24 1.0864716639004044 1109 25 1.0861965490042635 1110 1 1.1259160289516004 1110 2 3.561520601497019 1110 3 2.259416196396362 1110 4 1.5063741012599436 1110 5 0.7535835852936401 1110 6 0.6958386006876793 1110 7 0.618568773096861 1110 8 0.4630630480541811 1110 9 0.5225652255845006 1110 10 0.4189750877193461 1110 11 0.4512110144711343 1110 12 0.4996028757839923 1110 13 0.4807175995078339 1110 14 0.5591613384300942 1110 15 0.6475978875090362 1110 16 0.7325109414958462 1110 17 0.6867182107257364 1110 18 0.7336215541562652 1110 19 0.6928086579893034 1110 20 0.4864817219400095 1110 21 0.5363275632430842 1110 22 0.5488457828565814 1110 23 0.6373062235141518 1110 24 0.5488697329007479 1110 25 0.6206015832284137 1111 1 0.2534731451656107 1111 2 2.671176447166676 1111 3 1.2608382735615358 1111 4 0.3450222537252685 1111 5 -0.033451616944383034 1111 6 -0.2207265584217737 1111 7 -0.6692095227418164 1111 8 -0.5267696830333577 1111 9 -0.730412977003212 1111 10 -0.7931213104622614 1111 11 -0.7534559047502185 1111 12 -0.8855017401386078 1111 13 -0.8654969942676658 1111 14 -0.9822522878666788 1111 15 -1.0643149228418238 1111 16 -1.048770384999088 1111 17 -1.0632701329040586 1111 18 -1.1416654839600682 1111 19 -1.0150725053164464 1111 20 -1.1753771776986426 1111 21 -1.2608821931387766 1111 22 -1.4204659084827398 1111 23 -1.4107398774300108 1111 24 -1.3140688380282626 1111 25 -1.4749194565198067 1112 1 1.1103785984472196 1112 2 3.3638158063163957 1112 3 2.025468181402868 1112 4 1.322519899306812 1112 5 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-2.758595345847869 2316 1 -1.3236186596514052 2316 2 1.0537460436633914 2316 3 -0.2404285390753138 2316 4 -1.2279680496841792 2316 5 -1.401812580347574 2316 6 -1.6394081348997247 2316 7 -2.0211644805147273 2316 8 -1.8554997424640725 2316 9 -2.072843380506498 2316 10 -2.2857120205029204 2316 11 -2.125609486232861 2316 12 -2.434035521808923 2316 13 -2.219848730270165 2316 14 -2.479108715767836 2316 15 -2.579090493689492 2316 16 -2.715039543585263 2316 17 -2.581693654955984 2316 18 -2.8055222246544673 2316 19 -2.8303211759451585 2316 20 -2.389570171129982 2316 21 -2.818584726764051 2316 22 -2.5523317201812077 2316 23 -2.699702185844153 2316 24 -2.693455763270677 2316 25 -2.8921074452592492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82869.0 3 5.0 4 743.0 5 3508.0 6 4236.0 7 3734.0 8 3440.0 9 3348.0 10 3171.0 11 3121.0 12 2916.0 13 3042.0 14 3005.0 15 3070.0 16 3381.0 17 3549.0 18 4148.0 19 4880.0 20 6461.0 21 8361.0 22 10184.0 23 12109.0 24 14286.0 25 15798.0 26 17817.0 27 20527.0 28 23170.0 29 27491.0 30 34136.0 31 42796.0 32 54375.0 33 70839.0 34 95507.0 35 134175.0 36 206372.0 37 265327.0 38 605899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.761109470772496 19.714107479426087 14.147550555112787 24.377232494688634 2 15.814546216336828 21.590043239046175 40.67882575029987 21.91658479431712 3 18.55143423561824 25.31352050953604 30.00467311504743 26.13037213979829 4 11.86205319581129 17.891537110749496 39.93210108136548 30.31430861207373 5 12.885753992127855 38.04281949787878 36.10075724443833 12.970669265555035 6 29.816860510290844 38.44525129370916 19.09383748215669 12.6440507138433 7 26.90576513656374 32.14831201756469 22.89865223366019 18.04727061221137 8 23.986566736744894 37.68245683751102 21.36135278355596 16.96962364218813 9 25.64835308769694 16.390923278772956 21.141627576040793 36.819096057489304 10 14.428381459388298 29.96787649656232 34.79195203008554 20.811790013963847 11 33.02138532886076 23.598953488630233 25.213731188214428 18.165929994294583 12 22.73276219949428 25.284882417321384 32.47565207159967 19.506703311584666 13 28.184211753161847 22.7307641930607 26.479745764781367 22.605278288996093 14 20.361517064084946 23.095511367546603 28.683990862450575 27.858980705917872 15 22.375396548776887 31.327519874613998 24.266232137267483 22.030851439341635 16 21.130805041192232 29.412985709813988 28.461990147608272 20.994219101385507 17 20.91551984797391 29.163401406152527 28.54068940101987 21.38038934485369 18 20.503264520511756 28.447782101858365 30.71124589021177 20.33770748741811 19 22.496886439974336 27.64275200966147 28.677996843149835 21.182364707214358 20 22.388605591310004 28.109231011723857 29.375911590435322 20.126251806530817 21 22.278067921344885 27.552877971350714 28.269609665943314 21.89944444136109 22 21.886166028964656 28.63326417957333 28.808923610070476 20.671646181391534 23 21.67243318004065 28.26202059960639 28.688653788305135 21.37689243204783 24 21.876949443777207 27.650688535217082 29.53713960958954 20.935222411416166 25 21.93300462427489 28.012993701839722 29.06233558072057 20.99166609316482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 177.0 2 343.0 3 509.0 4 509.0 5 509.0 6 1364.0 7 2219.0 8 2219.0 9 2219.0 10 3407.5 11 4596.0 12 4596.0 13 4596.0 14 7563.5 15 10531.0 16 10531.0 17 10531.0 18 18122.5 19 25714.0 20 25714.0 21 25714.0 22 40638.5 23 55563.0 24 55563.0 25 55563.0 26 78665.0 27 101767.0 28 101767.0 29 101767.0 30 130375.0 31 158983.0 32 158983.0 33 158983.0 34 176579.0 35 194175.0 36 194175.0 37 194175.0 38 204673.5 39 215172.0 40 215172.0 41 215172.0 42 223617.0 43 232062.0 44 232062.0 45 232062.0 46 231653.0 47 231244.0 48 231244.0 49 231244.0 50 217951.0 51 204658.0 52 204658.0 53 204658.0 54 177314.5 55 149971.0 56 149971.0 57 149971.0 58 126216.0 59 102461.0 60 102461.0 61 102461.0 62 81332.5 63 60204.0 64 60204.0 65 60204.0 66 45435.0 67 30666.0 68 30666.0 69 30666.0 70 22169.5 71 13673.0 72 13673.0 73 13673.0 74 9326.0 75 4979.0 76 4979.0 77 4979.0 78 3340.0 79 1701.0 80 1701.0 81 1701.0 82 1108.0 83 515.0 84 515.0 85 515.0 86 344.0 87 173.0 88 173.0 89 173.0 90 115.0 91 57.0 92 57.0 93 57.0 94 39.5 95 22.0 96 22.0 97 22.0 98 13.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.361481543970572 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 3.330010722634527E-4 22 7.770025019480562E-4 23 7.770025019480562E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1801796.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.06207139086396 #Duplication Level Percentage of deduplicated Percentage of total 1 86.97111493354409 67.89145382885353 2 9.823773317581121 15.337281880893638 3 1.8520703681769433 4.337293479045967 4 0.5870224223832569 1.8329674497647874 5 0.2577073779395204 1.0058585867333603 6 0.12690947504334976 0.5944089900606623 7 0.07303903107428332 0.3991104640628165 8 0.05645775388616113 0.35257673715434695 9 0.037263415826221405 0.261797348384455 >10 0.18508503741891 2.649051350847758 >50 0.014682682561983492 0.8201235310462306 >100 0.01228794962860875 1.9706007063659763 >500 0.0015804769050483081 0.8501119035274346 >1k 9.339181711649085E-4 1.367334813101517 >5k 7.183985932037758E-5 0.33002893015759277 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5885 0.3266185517117365 No Hit GGTATCAACGCAGAGTACTTTTTTT 3418 0.18969961083274686 No Hit TATCAACGCAGAGTACTTTTTTTTT 3265 0.18120808349002884 No Hit GTCCTACAGTGGACATTTCTAAATT 2707 0.15023898376952774 No Hit CTGTAGGACGTGGAATATGGCAAGA 2640 0.14652047179591918 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2121 0.11771587904513053 No Hit CTTTAGGACGTGAAATATGGCGAGG 1963 0.1089468508088596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1240 0.0 12.917829 1 TCCAACG 140 3.6379788E-12 12.8336525 18 AACCGTG 55 0.0031763746 12.035388 7 CGTCAGA 55 0.0031763746 12.035388 14 CCAACGA 95 1.0995027E-6 11.944897 19 GACCTAC 125 1.0137228E-8 11.865264 1 ATAGGAC 245 0.0 11.579235 3 GCGACTA 60 0.0060810247 11.032439 16 AACACCG 60 0.0060810247 11.032439 5 CTAGACT 275 0.0 11.003782 4 TAGGACC 585 0.0 10.992027 4 TAGGACA 520 0.0 10.729349 4 GGCGAGG 585 0.0 10.345437 19 AATCCCG 260 0.0 10.183789 19 TAGCACC 125 2.7255064E-6 9.83463 4 GTAGAAC 640 0.0 9.751887 3 GTAGGAC 1550 0.0 9.700411 3 CCAGTAC 255 0.0 9.641794 3 AGGACGT 1510 0.0 9.581626 5 GTATCAA 2400 0.0 9.475732 1 >>END_MODULE