FastQCFastQC Report
Thu 26 May 2016
SRR1511715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70460.3910542591947146No Hit
GGTATCAACGCAGAGTACTTTTTTT42210.23426625433733897No Hit
TATCAACGCAGAGTACTTTTTTTTT40790.22638522896043725No Hit
GTCCTACAGTGGACATTTCTAAATT32460.1801535800945279No Hit
CTGTAGGACGTGGAATATGGCAAGA30120.16716653827625325No Hit
GTCCTAAAGTGTGTATTTCTCATTT23460.13020341925501No Hit
CTTTAGGACGTGAAATATGGCGAGG23130.128371913357561No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19490.10816984830691154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT1403.6379788E-1212.88892310
TACACCG701.0905205E-412.2149655
GGTATCA13600.012.0632611
TAGGACC4900.012.0210754
CCAACGA2000.011.87170419
GGCGAGG6900.011.83729419
TCCAACG2550.010.80062318
ACTGTTC6300.010.5523348
GATATAC6650.010.3272831
CCCGTTT1856.002665E-1110.26713612
GTAGAAC6050.010.2071783
AGGACCT8750.010.2062815
GTCCTAA9450.010.1944721
CGAAATC1502.4561814E-810.1302413
CACTGCG750.002654636710.130247
TTAGGAC10000.010.0705593
CAGACCG856.590664E-410.05938155
CTAGACT2400.09.8963834
TAGACTC1252.5680256E-69.8805485
CCGTTTC1951.6370905E-109.74061513
Produced by FastQC (version 0.11.5)