FastQCFastQC Report
Thu 26 May 2016
SRR1511714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183977
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53372.9009060915223097No Hit
GGTATCAACGCAGAGTACTTTTTTT36812.000793577458052No Hit
TATCAACGCAGAGTACTTTTTTTTT36802.000250031253907No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17280.9392478407627041No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15700.853367540507781No Hit
GTACTGGTTCACTATCGGTCAGTCA14040.763138870619697No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12680.6892165868559657No Hit
GGATACCACGTGTCCCGCCCTACTC10030.5451768427575187No Hit
ATCATTAACTGAATCCATAGGTTAA9470.5147382553253939No Hit
ATACAGGGTGACAGCCCCGTACACA9110.4951705919761709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7480.4065725607005223No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6840.371785603635237No Hit
GTACATGGGGGTTAAGCGACTAAGC6730.3658065953896411No Hit
GGTTAATGAGGCGAACCGGGGGAAC6580.35765340232746484No Hit
GTATTTAGCCTTGGAGGATGGTCCC6180.3359115541616615No Hit
GTGGTATCAACGCAGAGTACATGGG6020.32721481489534016No Hit
TATTCAGACAGGATACCACGTGTCC5890.3201487142414541No Hit
GGTATCAACGCAGAGTACATGGGGG5610.3049294205253918No Hit
GTGGATGCCCTGGCAGTCAGAGGCG5560.30221168950466637No Hit
CCCATATTCAGACAGGATACCACGT5410.2940584964424901No Hit
GTGGTATCAACGCAGAGTACTTTTT5290.28753594199274907No Hit
ATCAACGCAGAGTACTTTTTTTTTT5280.28699239578860397No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5200.2826440261554433No Hit
AGCGTACACGGTGGATGCCCTGGCA5130.27883920272642776No Hit
GTTAATGATAGTGTGTCGAAACACA5030.2734037406849769No Hit
GTTCACTATCGGTCAGTCAGGAGTA4880.26525054762280065No Hit
CTATCGGTCAGTCAGGAGTATTTAG4840.2630763628062203No Hit
GTATCAACGCAGAGTACATGGGGGT4840.2630763628062203No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4830.2625328166020752No Hit
ACTTAGATGTTTCAGTTCCCCCGGT4590.24948770770259324No Hit
GCCTTGGAGGATGGTCCCCCCATAT4550.2473135228860129No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4450.2418780608445621No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4320.23481196019067602No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4100.22285394369948416No Hit
GTTATAACCGGCGATTTCCGAATGG3870.21035238100414727No Hit
GTGCTAATCTGCGATAAGCGTCGGT3730.2027427341461161No Hit
CGGTACTGGTTCACTATCGGTCAGT3690.20056854932953574No Hit
CATCTAAGTACCCCGAGGAAAAGAA3650.19839436451295542No Hit
GTACTTAGATGTTTCAGTTCCCCCG3560.19350244867564967No Hit
GGTTCACTATCGGTCAGTCAGGAGT3520.19132826385906934No Hit
TCACGGTACTGGTTCACTATCGGTC3270.17773960875544226No Hit
GATATGAACCGTTATAACCGGCGAT3190.17339123912228158No Hit
ACGCAGAGTACATGGGGGTTAAGCG3150.17121705430570125No Hit
GTACATAAGCAGTGGTATCAACGCA3120.16958641569326602No Hit
TATCAACGCAGAGTACATGGGGGTT3100.16849932328497585No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3030.16469449985596027No Hit
GGAGTATTTAGCCTTGGAGGATGGT3000.163063861243525No Hit
GGTGATATGAACCGTTATAACCGGC2970.16143322263108975No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2960.16088967642694466No Hit
CACACACACTGATTCAGGCTCTGGG2940.1598025840186545No Hit
GGTATCAACGCAGAGTACATGGGGA2900.15762839920207417No Hit
GAGTACATGGGGGTTAAGCGACTAA2870.15599776058963893No Hit
TCATTAACCTATGGATTCAGTTAAT2760.15001875234404302No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2690.1462139289150274No Hit
CTATCATTAACTGAATCCATAGGTT2680.14567038271088234No Hit
CACTATCATTAACTGAATCCATAGG2670.14512683650673724No Hit
GNATCAACGCAGAGTACTTTTTTTT2660.14458329030259218No Hit
ATCCATAGGTTAATGAGGCGAACCG2660.14458329030259218No Hit
GGGTGACAGCCCCGTACACAAAAAT2660.14458329030259218No Hit
GTCCCGCCCTACTCATCGAGCTCAC2640.14349619789430199No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2630.14295265169015692No Hit
CCGTTATAACCGGCGATTTCCGAAT2540.13806073585285117No Hit
GTGGTATCCTGTCTGAATATGGGGG2500.13588655103627084No Hit
TCGGTAAGGTGATATGAACCGTTAT2350.12773335797409457No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2340.1271898117699495No Hit
GCCCTACTCATCGAGCTCACAGCAT2300.12501562695336918No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2300.12501562695336918No Hit
GGATCAACGCAGAGTACTTTTTTTT2290.1244720807492241No Hit
TTCCAGACGCTTCCACTAACACACA2210.12012371111606343No Hit
AGCTCACAGCATGTGCATTTTTGTG2180.11849307250362817No Hit
CCTATGGATTCAGTTAATGATAGTG2160.11740598009533801No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2150.11686243389119291No Hit
GGGTACTTAGATGTTTCAGTTCCCC2120.11523179527875767No Hit
TTTGTGTACGGGGCTGTCACCCTGT2110.11468824907461259No Hit
GCGTACACGGTGGATGCCCTGGCAG2100.1141447028704675No Hit
CAACGCAGAGTACTTTTTTTTTTTT2040.11088342564559701No Hit
CCCATGTACTCTGCGTTGATACCAC1990.10816569462487159No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1980.10762214842072651No Hit
GTCCCCCCATATTCAGACAGGATAC1970.10707860221658141No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1970.10707860221658141No Hit
GAATATGGGGGGACCATCCTCCAAG1920.10436087119585599No Hit
CATTAACTGAATCCATAGGTTAATG1900.10327377878756583No Hit
GGGTTTCCCCATTCGGAAATCGCCG1890.10273023258342076No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1880.10218668637927567No Hit
AACGCAGAGTACTTTTTTTTTTTTT1880.10218668637927567No Hit
GAATCAACGCAGAGTACTTTTTTTT1870.10164314017513057No Hit
TAAGTACCCCGAGGAAAAGAAATCA1870.10164314017513057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCA250.00480213619.7527681
TAGGACG250.006169683818.9151954
GGATCAA455.0039374E-415.3632631
GGCAGAG400.004059436314.8145762
CGAATGG500.001541908413.24063719
ATATCAA450.00886032613.1685112
AGGCAGA450.00886032613.1685111
CCCCGGT604.217823E-412.6101319
GGTATCA10700.012.2762531
TGGGTAA550.003154737212.0369426
TGTCTGA658.269127E-411.6401210
GATCAAC600.00437194111.5224482
TAAGGTG1002.004137E-611.3491175
ATTAACC752.1421038E-411.3491163
TTAACCT752.1421038E-411.3491164
GTGTCCC1252.3322718E-710.59250910
AGGGTGA1803.6379788E-1110.5084415
ACAGGGT1803.6379788E-1110.5084413
AAGGTGA1106.2772415E-610.3173786
TAATGAT1106.2772415E-610.3173783
Produced by FastQC (version 0.11.5)