FastQCFastQC Report
Thu 26 May 2016
SRR1511714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183977
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65393.5542486289047No Hit
GGTATCAACGCAGAGTACTTTTTTT44062.394864575463237No Hit
TATCAACGCAGAGTACTTTTTTTTT43782.379645281747175No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21971.1941710105067482No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18441.0022992004435338No Hit
GTACTGGTTCACTATCGGTCAGTCA16130.8767400272860194No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14080.7653130554362774No Hit
ATCATTAACTGAATCCATAGGTTAA11280.613120118275654No Hit
ATACAGGGTGACAGCCCCGTACACA11060.6011621017844622No Hit
GGATACCACGTGTCCCGCCCTACTC10970.5962701859471564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8310.45168689564456427No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8290.4505998032362741No Hit
GGTTAATGAGGCGAACCGGGGGAAC8170.44407724878653315No Hit
GTGGTATCAACGCAGAGTACATGGG7640.4152692999668437No Hit
GTACATGGGGGTTAAGCGACTAAGC7380.4011370986590715No Hit
CCCATATTCAGACAGGATACCACGT6790.3690678726145116No Hit
GGTATCAACGCAGAGTACATGGGGG6780.36852432641036653No Hit
GTATTTAGCCTTGGAGGATGGTCCC6700.3641759567772059No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6480.352217940286014No Hit
GTGGTATCAACGCAGAGTACTTTTT6330.34406474722383773No Hit
AGCGTACACGGTGGATGCCCTGGCA6300.3424341086114025No Hit
TATTCAGACAGGATACCACGTGTCC6020.32721481489534016No Hit
GTTAATGATAGTGTGTCGAAACACA6000.32612772248705No Hit
ATCAACGCAGAGTACTTTTTTTTTT5970.32449708387461473No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5930.3223228990580344No Hit
GTATCAACGCAGAGTACATGGGGGT5730.3114519749751328No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5550.3016681433005213No Hit
GTTCACTATCGGTCAGTCAGGAGTA5340.2902536730134745No Hit
ACTTAGATGTTTCAGTTCCCCCGGT5340.2902536730134745No Hit
CTATCGGTCAGTCAGGAGTATTTAG5220.2837311185637335No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4980.2706860096642515No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4880.26525054762280065No Hit
GCCTTGGAGGATGGTCCCCCCATAT4870.2647070014186556No Hit
GTTATAACCGGCGATTTCCGAATGG4380.23807323741554653No Hit
CGGTACTGGTTCACTATCGGTCAGT4190.2277458595367899No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4150.2255716747202096No Hit
GTGCTAATCTGCGATAAGCGTCGGT4060.22067975888290384No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3930.21361365822901776No Hit
GGTTCACTATCGGTCAGTCAGGAGT3900.2119830196165825No Hit
TATCAACGCAGAGTACATGGGGGTT3790.20600401137098656No Hit
CATCTAAGTACCCCGAGGAAAAGAA3700.20111209553368084No Hit
GTACTTAGATGTTTCAGTTCCCCCG3680.20002500312539068No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3670.19948145692124558No Hit
ACGCAGAGTACATGGGGGTTAAGCG3660.1989379107171005No Hit
GGTGATATGAACCGTTATAACCGGC3650.19839436451295542No Hit
CACTATCATTAACTGAATCCATAGG3560.19350244867564967No Hit
TCACGGTACTGGTTCACTATCGGTC3520.19132826385906934No Hit
TCATTAACCTATGGATTCAGTTAAT3510.19078471765492425No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT3450.18752344043005376No Hit
GATATGAACCGTTATAACCGGCGAT3410.18534925561347343No Hit
GGTATCAACGCAGAGTACATGGGGA3340.18154443218445784No Hit
CACACACACTGATTCAGGCTCTGGG3310.17991379357202258No Hit
GAGTACATGGGGGTTAAGCGACTAA3230.17556542393886193No Hit
GTACATAAGCAGTGGTATCAACGCA3200.17393478532642667No Hit
GGAGTATTTAGCCTTGGAGGATGGT3190.17339123912228158No Hit
ATCCATAGGTTAATGAGGCGAACCG3100.16849932328497585No Hit
CTATCATTAACTGAATCCATAGGTT2990.16252031503937991No Hit
GTGGTATCCTGTCTGAATATGGGGG2970.16143322263108975No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2810.15273648336476844No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2770.1505622985481881No Hit
GGGTACTTAGATGTTTCAGTTCCCC2740.14893165993575283No Hit
CCGTTATAACCGGCGATTTCCGAAT2700.1467574751191725No Hit
TCGGTAAGGTGATATGAACCGTTAT2630.14295265169015692No Hit
GCCCTACTCATCGAGCTCACAGCAT2590.1407784668735766No Hit
GGGTGACAGCCCCGTACACAAAAAT2550.13860428205699626No Hit
AGCTCACAGCATGTGCATTTTTGTG2540.13806073585285117No Hit
TTTGTGTACGGGGCTGTCACCCTGT2510.13643009724041594No Hit
CAACGCAGAGTACTTTTTTTTTTTT2490.13534300483212575No Hit
GCGTACACGGTGGATGCCCTGGCAG2460.13371236621969051No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2420.13153818140311016No Hit
GTCCCGCCCTACTCATCGAGCTCAC2400.13045108899482No Hit
GGTATCAACGCAGAGTACATGGGCA2380.12936399658652983No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2350.12773335797409457No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2280.123928534545079No Hit
CCTATGGATTCAGTTAATGATAGTG2280.123928534545079No Hit
TAAGTACCCCGAGGAAAAGAAATCA2270.12338498834093392No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2190.11903661870777327No Hit
TTCCAGACGCTTCCACTAACACACA2170.11794952629948308No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2170.11794952629948308No Hit
GTATCAACGCAGAGTACATGGGGAA2160.11740598009533801No Hit
GTCCCCCCATATTCAGACAGGATAC2130.11577534148290275No Hit
GTATCAACGCAGAGTACATGGGGAG2120.11523179527875767No Hit
CATTAACTGAATCCATAGGTTAATG2110.11468824907461259No Hit
GGGTTTCCCCATTCGGAAATCGCCG2100.1141447028704675No Hit
AACGCAGAGTACTTTTTTTTTTTTT2090.11360115666632242No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2080.11305761046217734No Hit
GAATATGGGGGGACCATCCTCCAAG2070.11251406425803225No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2050.11142697184974208No Hit
TAATGATAGTGTGTCGAAACACACT2050.11142697184974208No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2010.10925278703316176No Hit
ATGATAGTGTGTCGAAACACACTGG1970.10707860221658141No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1940.10544796360414618No Hit
ATTTAGCCTTGGAGGATGGTCCCCC1930.10490441740000109No Hit
GTGTTTCGACACACTATCATTAACT1930.10490441740000109No Hit
TCAACGCAGAGTACTTTTTTTTTTT1910.10381732499171092No Hit
GGGAAACCCAGTGTGTTTCGACACA1900.10327377878756583No Hit
GTACATGGGCATCTAAGTACCCCGA1900.10327377878756583No Hit
GCTTATGTACTCTGCGTTGATACCA1890.10273023258342076No Hit
CCCATGTACTCTGCGTTGATACCAC1870.10164314017513057No Hit
GAGTAGGGCGGGACACGTGGTATCC1860.1010995939709855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCTA307.710379E-418.9915759
GGCATCT350.002166844416.2784928
AGCCTGA1155.2277755E-912.3858097
TACATGA701.0770958E-412.2188332
TACTTAG1405.0931703E-1112.2188332
GGTATCA12500.012.1413451
CCCCGGT1209.833457E-911.86973519
CCAGAGC1302.5283953E-911.696663
CAGAGCC1302.5283953E-911.696664
CCCCCGG1302.561137E-911.68712218
GAGCCTG1251.7991624E-811.3949446
GTACTTA1451.0913936E-911.1907861
TTCCGAA600.00586167611.07841916
CTGACCG600.00586167611.0784199
CGAATGG600.00586167611.07841919
TCCGAAT600.00586167611.07841917
CCGAATG600.00586167611.07841918
CGCCCTA2250.010.9729116
CCCGCCC2250.010.9729114
CCCAGAG1303.169771E-810.9656192
Produced by FastQC (version 0.11.5)