FastQCFastQC Report
Thu 26 May 2016
SRR1511713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099395
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49480.23568694790642064No Hit
TATCAACGCAGAGTACTTTTTTTTT29690.14142169529793108No Hit
GGTATCAACGCAGAGTACTTTTTTT29590.1409453675939973No Hit
GTCCTACAGTGGACATTTCTAAATT26250.12503602228261No Hit
CTGTAGGACGTGGAATATGGCAAGA22470.10703083507391416No Hit
GTCCTAAAGTGTGTATTTCTCATTT22270.10607817966604666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2000.012.2940219
TATACCG701.1385146E-412.1589225
TCCAACG2900.012.0657518
ACCGTAA550.003175259312.0361058
AGGACCG752.1638563E-411.3483285
GGTATCA10400.010.5476561
TAGGACC5050.010.4869034
GTATCAA20400.010.4638111
CTTATAC1909.094947E-1210.4524073
CCAACGT1401.0357144E-710.13243519
ACTGTTC7250.010.0439218
ATACCGT856.8761955E-410.0132296
TATGTCG1054.316175E-59.90726916
GTCCTAC14200.09.8824881
TGTAGGA14350.09.7791872
GTAGGAC15000.09.772173
GGCGAGG6700.09.73923619
TGGCGAG14450.09.62055418
GATATAC7350.09.5463491
TGAGTTA7050.09.5240112
Produced by FastQC (version 0.11.5)