FastQCFastQC Report
Thu 26 May 2016
SRR1511713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099395
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60750.28936908013975454No Hit
TATCAACGCAGAGTACTTTTTTTTT36060.1717637700385111No Hit
GGTATCAACGCAGAGTACTTTTTTT35630.16971556091159595No Hit
GTCCTACAGTGGACATTTCTAAATT28550.1359915594730863No Hit
CTGTAGGACGTGGAATATGGCAAGA26340.12546471721615035No Hit
GTCCTAAAGTGTGTATTTCTCATTT24270.1156047337447217No Hit
CTTTAGGACGTGAAATATGGCGAGG21570.10274388573851037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.012.5646451
TCCAACG2700.012.31077118
GGCGAGG6300.011.75832219
GTCTACG657.7486906E-411.741321
AGCGGTT600.00589120711.07969418
TGCCGGT2150.011.04288510
TAGGACC5600.011.0278924
CCAACGA2001.8189894E-1210.44681719
GTCCTAC14350.010.4372971
CGGTTTC2100.010.40134513
GCCGGTT2200.010.36023211
GGCGTGC1201.5306196E-610.2882878
ACTGTTC7150.010.2274098
GTAGGAC15300.010.0598333
GGACCGT951.6499052E-49.9967166
TGGCGAG13900.09.9751418
TTAGGAC10350.09.9140393
AATCCCG3450.09.91002419
CCAACGT1252.580633E-69.87698919
TGTAGGA16800.09.6706132
Produced by FastQC (version 0.11.5)