FastQCFastQC Report
Thu 26 May 2016
SRR1511712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478442
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50780.20488677967852384No Hit
GGTATCAACGCAGAGTACTTTTTTT30100.12144726404733296No Hit
TATCAACGCAGAGTACTTTTTTTTT29970.1209227409800189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.014.1227461
TCCAACG1600.012.4119418
TAGGACC3900.012.123754
CGCCTAT600.004367872611.5335761
CCAACGA1407.348717E-1011.48315319
GTATTAC1751.1459633E-1010.7332871
CGCCCTA1304.0924533E-710.184154516
GTATCAA26050.010.1325961
GTCCTAC10400.09.8859221
TAGGACG10350.09.7763114
GTAGGAC9150.09.7148993
GTCCTAA7000.09.6034671
AGGACGT10300.09.5483355
TTAGGAC7450.09.5199913
TCCGAAT800.0046904049.45671617
CGGGACC800.00469118459.4565254
GTCTTAG3050.09.399731
TGTAGGA9950.09.3394642
GGACGTG10550.09.3220716
CTAAATT7300.09.19762619
Produced by FastQC (version 0.11.5)