FastQCFastQC Report
Thu 26 May 2016
SRR1511712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478442
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62560.2524166391628289No Hit
TATCAACGCAGAGTACTTTTTTTTT37580.15162751438201902No Hit
GGTATCAACGCAGAGTACTTTTTTT36170.1459384564980742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.012.9437641
GTATACG550.002971848212.143121
TAGGACC4150.010.9884074
GTCCTAA6050.010.8814961
GACGTGA5200.010.5927457
TTAGGAC6900.010.18882753
TAGGACG10950.09.9775834
GTATCAA28600.09.9079851
TGCACCG1356.4427877E-79.852265
GTCTAGG2401.8189894E-129.5410221
GTATAAG2600.09.5410221
AAGACCG900.00111358359.5003935
ATGACCG900.00111358359.5003935
TCCAACG1304.2579322E-69.49694318
AGGACGT11200.09.3307445
GAACAGT4800.09.299096
GTACTAG1752.4901965E-89.2684211
TTTAGGA8400.09.1610932
CTACGAC1252.7537213E-59.1203783
GGACGTG11600.09.0875936
Produced by FastQC (version 0.11.5)