FastQCFastQC Report
Thu 26 May 2016
SRR1511711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087702
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT56170.2690518091183512No Hit
GTATCAACGCAGAGTACTTTTTTTT54500.2610525831751849No Hit
CTGTAGGACGTGGAATATGGCAAGA49170.23552211953621732No Hit
GTCCTAAAGTGTGTATTTCTCATTT46920.2247447193133886No Hit
CTTTAGGACGTGAAATATGGCGAGG39620.18977804303487758No Hit
TATCAACGCAGAGTACTTTTTTTTT32500.15567355877419287No Hit
GGTATCAACGCAGAGTACTTTTTTT31920.15289538449453033No Hit
CTGAAGGACCTGGAATATGGCGAGA22810.10925888848121044No Hit
TTGTAGAACAGTGTATATCAATGAG22490.10772610267174147No Hit
GTCCTACAGTGTGCATTTCTCATTT22360.1071034084366447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCT457.000024E-414.71097912
TCCAACG3350.012.7035118
CGGTAGG801.915598E-512.3517291
GGCGAGG12650.011.96149619
GCGCGCC658.3362556E-411.63945611
TAGGACC7750.011.5925234
AAATGTC6600.011.3198127
GGTATCA12350.011.2015691
GTCCTAA13900.011.1609891
GGACGTC855.5760614E-511.1259516
TGTATCG700.001549219210.8080655
AGGACGT29500.010.8034875
TTAGGAC17600.010.6929243
GTCCTAC26850.010.6726311
GGACGTG29500.010.611146
AATGTCC7250.010.5658168
CCAACGA2150.010.55671519
AAGACCG909.964976E-510.5078425
ACTGTTC9950.010.4550398
ATTTCTA21550.010.44445415
Produced by FastQC (version 0.11.5)