FastQCFastQC Report
Thu 26 May 2016
SRR1511711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087702
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66960.3207354306313832No Hit
GTCCTACAGTGGACATTTCTAAATT64800.31038912641746763No Hit
CTGTAGGACGTGGAATATGGCAAGA57330.2746081576776762No Hit
GTCCTAAAGTGTGTATTTCTCATTT52580.2518558683183711No Hit
CTTTAGGACGTGAAATATGGCGAGG45290.216937091596406No Hit
GGTATCAACGCAGAGTACTTTTTTT41450.1985436618827783No Hit
TATCAACGCAGAGTACTTTTTTTTT40990.19634028228166664No Hit
GTCCTACAGTGTGCATTTCTCATTT27670.13253807296252051No Hit
CTGAAGGACCTGGAATATGGCGAGA26080.12492204347172155No Hit
TTGTAGAACAGTGTATATCAATGAG23720.11361774812688784No Hit
GATATACACTGTTCTACAAATCCCG21590.10341514258260998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7600.014.0008834
GGTATCA13300.012.7683171
TTCACGA550.003067603312.0916712
GGCGAGG12300.012.04492319
CTAGGAC2750.011.7461953
AAGCGAC658.047032E-411.68856414
TCCAACG3700.011.55035518
CCAACGT1803.6379788E-1211.07978519
TGTAGGA28800.010.9850682
GAAATGT6350.010.9177636
CTGTAGG28750.010.8179511
AAATGTC6250.010.7885457
AGGACCT18100.010.7078575
ACGAAAT2150.010.60125612
AGGACGT30550.010.5113015
GTAGGAC29300.010.4733563
TGGCGAG28350.010.418179518
GTCCTAC29650.010.2965221
GTCCTAA15050.010.269371
CCAACGA2500.010.25671519
Produced by FastQC (version 0.11.5)