FastQCFastQC Report
Thu 26 May 2016
SRR1511710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412524
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54650.22652624388399867No Hit
GGTATCAACGCAGAGTACTTTTTTT33530.13898307332901144No Hit
TATCAACGCAGAGTACTTTTTTTTT31330.12986399306286694No Hit
GTCCTACAGTGGACATTTCTAAATT25660.1063616361951218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2750.012.72353818
GGTATCA12500.012.0990651
GCACCGT1501.9099389E-1011.3480216
CTAGTAC1251.9614163E-811.3480213
ACCGTGC1251.9614163E-811.3480218
CCAACGA2350.011.26753819
TAGGACC5550.011.0753964
AGGACCG1053.6633282E-610.8076385
AATGCCG3950.010.773437518
GGACCGA1159.356918E-710.6901656
CCCCGTA803.9349718E-410.63876915
CCCGTAC803.9349718E-410.63876916
GATATAC9700.010.4962921
TAGACAG3450.010.4160585
GTGCTAC2103.6379788E-1210.3555651
GTCCTAC11550.010.2699811
GTATCAA25100.010.1999131
ACCGCAT750.002750784910.087138
GGACCGT856.878688E-410.01295956
TATACAC12550.09.9464733
Produced by FastQC (version 0.11.5)