##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511710_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2412524 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.963237671417986 31.0 28.0 34.0 19.0 34.0 2 26.329872780540214 31.0 25.0 34.0 10.0 34.0 3 27.98059584070459 31.0 27.0 34.0 16.0 34.0 4 32.417234813000825 35.0 32.0 37.0 23.0 37.0 5 32.858017578270726 35.0 33.0 37.0 26.0 37.0 6 33.105677290671515 37.0 35.0 37.0 27.0 37.0 7 33.136853768086866 37.0 35.0 37.0 26.0 37.0 8 33.15224221603599 37.0 35.0 37.0 26.0 37.0 9 34.70285559853498 39.0 35.0 39.0 27.0 39.0 10 34.57958511500818 39.0 35.0 39.0 27.0 39.0 11 34.586471264120064 39.0 35.0 39.0 27.0 39.0 12 34.48213820878051 39.0 35.0 39.0 27.0 39.0 13 34.4687957508402 39.0 35.0 39.0 27.0 39.0 14 35.58150385239691 40.0 36.0 41.0 27.0 41.0 15 35.51063657812316 40.0 36.0 41.0 26.0 41.0 16 35.44553919463599 40.0 36.0 41.0 26.0 41.0 17 35.412846877378215 40.0 36.0 41.0 26.0 41.0 18 35.34572547257561 40.0 36.0 41.0 25.0 41.0 19 35.37043693658592 40.0 36.0 41.0 25.0 41.0 20 35.28557643364377 40.0 36.0 41.0 25.0 41.0 21 35.19737171526584 40.0 36.0 41.0 25.0 41.0 22 35.08412351545518 40.0 36.0 41.0 24.0 41.0 23 34.97139468871605 40.0 35.0 41.0 23.0 41.0 24 34.85172789990897 40.0 35.0 41.0 22.0 41.0 25 34.73654106653447 39.0 35.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8308988551150556 1101 2 3.2927090292460903 1101 3 2.011738155365798 1101 4 1.1435374368887565 1101 5 0.6695845896843835 1101 6 0.4347973905848974 1101 7 0.3643822326089463 1101 8 0.17927881754693686 1101 9 0.16873140017463584 1101 10 0.4272485321391173 1101 11 0.20531824718426606 1101 12 0.189367303142987 1101 13 0.15556688402899255 1101 14 0.37116292941037443 1101 15 0.1734202330173389 1101 16 0.2769545878338633 1101 17 0.3163068227121002 1101 18 0.298106210469669 1101 19 0.29127313036030955 1101 20 0.4296963228201207 1101 21 0.2263101713359248 1101 22 0.020354327889734236 1101 23 0.1496499517114458 1101 24 -0.017399371233857153 1101 25 -0.06072259996165741 1102 1 1.718240144117381 1102 2 4.3906437743657705 1102 3 2.93020059587073 1102 4 2.0312489174249606 1102 5 1.4650323834808034 1102 6 1.2710375322233958 1102 7 1.1853881878640564 1102 8 1.2246233715925996 1102 9 1.4150991544226414 1102 10 1.295055894581921 1102 11 1.2860662180288074 1102 12 1.2490118272974087 1102 13 1.3743762928721992 1102 14 1.5063170992386148 1102 15 1.4979694500967327 1102 16 1.4176036876476203 1102 17 1.4719873083298154 1102 18 1.5430088340009718 1102 19 1.4204481320848004 1102 20 1.440868289433908 1102 21 1.4023219898730943 1102 22 1.3403975552241292 1102 23 1.2070255006755986 1102 24 1.120663676293212 1102 25 1.296790448715086 1103 1 1.3203893383958523 1103 2 3.965383046500481 1103 3 2.5750403568701863 1103 4 1.5468645248846755 1103 5 1.108281523440965 1103 6 0.8433695981296054 1103 7 0.7815795693596144 1103 8 0.6130236975024275 1103 9 0.8819260600898531 1103 10 0.7200414097005137 1103 11 0.8365270183185061 1103 12 0.8805526565568655 1103 13 0.8650118170438432 1103 14 1.0216674523075966 1103 15 0.8300063865443192 1103 16 0.7865091737964534 1103 17 0.8741057222352993 1103 18 0.8999606717703017 1103 19 1.0238158670114714 1103 20 0.9046081047516665 1103 21 0.9233178254696881 1103 22 1.0594587538480624 1103 23 0.8823450733715958 1103 24 1.1457179945984848 1103 25 0.6506425218637375 1104 1 1.6897917977657357 1104 2 4.3021226976115265 1104 3 2.791355459985379 1104 4 2.0219450205385954 1104 5 1.510532781561409 1104 6 1.3459464040414417 1104 7 1.1263838197363256 1104 8 1.1477994092919062 1104 9 1.3082340929891245 1104 10 1.2660405523401863 1104 11 1.3804128278882928 1104 12 1.235692265401255 1104 13 1.248933132327366 1104 14 1.3902503508040382 1104 15 1.5182412208023095 1104 16 1.2580936495609905 1104 17 1.4288848617479246 1104 18 1.4168241676355535 1104 19 1.4971410505535943 1104 20 1.5113685799251613 1104 21 1.51673702656106 1104 22 1.4971122067422158 1104 23 1.3982341594667247 1104 24 1.4071203285429092 1104 25 1.4427504685115267 1105 1 1.4997458780134458 1105 2 3.9400720900071597 1105 3 2.4030679702152753 1105 4 1.6750047259256178 1105 5 1.325046878087953 1105 6 1.021374744995704 1105 7 1.0201927238234703 1105 8 0.8349674053565082 1105 9 0.9541894046677726 1105 10 0.746568449469585 1105 11 0.8570953351367052 1105 12 0.9685838100744064 1105 13 0.7655823294497637 1105 14 0.7164577821903961 1105 15 0.8640354431904811 1105 16 0.8844498316558074 1105 17 1.0261118766889368 1105 18 0.9297113108735502 1105 19 0.9268364170317298 1105 20 0.9255312898175845 1105 21 0.9826131609520843 1105 22 0.9490857932767369 1105 23 1.0303132186275974 1105 24 1.00671048534808 1105 25 1.0145207637596343 1106 1 1.0486658550377896 1106 2 3.485807832922056 1106 3 2.1864600607874394 1106 4 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1107 17 1.0360918631952387 1107 18 1.0297479644357566 1107 19 1.0097209064119212 1107 20 1.2751669436782151 1107 21 1.2128887600594283 1107 22 1.2314431038783695 1107 23 1.3354468257273595 1107 24 1.5415358295023225 1107 25 1.4632888959200514 1108 1 1.5385683430502297 1108 2 4.0776023971383495 1108 3 2.6371622388788865 1108 4 1.5783565384860694 1108 5 1.2957924761013615 1108 6 1.1094589557610988 1108 7 0.9017165881459448 1108 8 0.9199178445614891 1108 9 0.8469183429860152 1108 10 0.7839484748698524 1108 11 0.7343668321433725 1108 12 0.8720461573084819 1108 13 0.8736091530352894 1108 14 0.8130552448139667 1108 15 0.9097574295591357 1108 16 0.8212974357599734 1108 17 0.8913470183872008 1108 18 0.7868848029932209 1108 19 0.7604813546761662 1108 20 0.9541633664499116 1108 21 0.8796410274246895 1108 22 0.8669051561503309 1108 23 0.6485879692148089 1108 24 0.9202710456680236 1108 25 0.9387314534467848 1109 1 1.3457509309691886 1109 2 3.2504531412756315 1109 3 2.2781883576970436 1109 4 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-0.4546917629689915 2314 9 -0.6583576476094493 2314 10 -0.6298150098906063 2314 11 -0.787690574357292 2314 12 -0.7360690382696191 2314 13 -0.7788869462519443 2314 14 -0.973245937319696 2314 15 -1.1033028562599725 2314 16 -0.7629586736912444 2314 17 -0.675442034778122 2314 18 -0.7639639321480232 2314 19 -0.6687917637384828 2314 20 -0.7763170100741519 2314 21 -0.9689596409426358 2314 22 -0.8890162899898542 2314 23 -0.7747442173399008 2314 24 -0.7201774542170583 2314 25 -0.7986598583489979 2315 1 -2.0098226584962617 2315 2 -3.9407077040340788 2315 3 -1.8336573461214662 2315 4 -1.9119833488280378 2315 5 -1.8184471445594355 2315 6 -1.8535692463221771 2315 7 -1.7516733455350924 2315 8 -1.773037513096913 2315 9 -1.868844812107703 2315 10 -2.0850705567883594 2315 11 -1.9505919872078792 2315 12 -2.0251385041085967 2315 13 -1.9126410530592608 2315 14 -2.1001309804868598 2315 15 -2.185662853762615 2315 16 -2.3163271166583073 2315 17 -2.1909234235838824 2315 18 -2.333838813251411 2315 19 -2.3169584396258145 2315 20 -2.3240750659931635 2315 21 -2.3644147186306483 2315 22 -2.199206637884508 2315 23 -2.242368566921826 2315 24 -2.203150548310745 2315 25 -2.4196482294215187 2316 1 -0.6039930250123398 2316 2 1.6163909008025534 2316 3 0.30203967633050155 2316 4 -0.7532174208344813 2316 5 -0.973686828341858 2316 6 -1.0886997419966349 2316 7 -1.4534131257648468 2316 8 -1.4356721949098628 2316 9 -1.7274624219320387 2316 10 -1.6536961579632319 2316 11 -1.6777925668644613 2316 12 -1.8498387474152338 2316 13 -1.8160342161589966 2316 14 -1.9271968580684486 2316 15 -2.0042144579492387 2316 16 -2.135483340266994 2316 17 -2.0007548919985965 2316 18 -2.2486824632075653 2316 19 -2.113295710279729 2316 20 -1.822382942093185 2316 21 -2.144262574987337 2316 22 -2.0175663199811282 2316 23 -2.246964647107653 2316 24 -2.063408573296279 2316 25 -2.0756233443883687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104505.0 3 2.0 4 914.0 5 4306.0 6 5208.0 7 4774.0 8 4396.0 9 4176.0 10 4097.0 11 3898.0 12 3819.0 13 3764.0 14 3770.0 15 3962.0 16 4152.0 17 4495.0 18 5219.0 19 6287.0 20 8090.0 21 10234.0 22 12775.0 23 15468.0 24 17537.0 25 20052.0 26 22462.0 27 25461.0 28 29439.0 29 35289.0 30 43420.0 31 55403.0 32 69998.0 33 91634.0 34 124724.0 35 175595.0 36 277786.0 37 353252.0 38 852161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.431526484296114 18.456935557946778 13.099931855600195 25.011606102156914 2 16.545550097980726 20.913501843718684 39.650154867170365 22.89079319113022 3 19.01183159214167 24.3844206316704 29.382754327003585 27.220993449184338 4 12.43407319471226 16.768247694116205 38.166128088259434 32.631551022912106 5 13.569315787117558 37.346695825616656 35.558402735060874 13.52558565220491 6 31.76084465895469 36.93936309027392 18.240357401625847 13.059434849145543 7 27.999555652088848 31.41684808109681 22.1693960350239 18.41420023179044 8 25.119998806229493 36.92394355455117 20.483319544178627 17.472738095040715 9 26.263697273063396 15.801666636269731 20.065168263611056 37.869467827055814 10 15.0377364121559 29.19266295381932 34.01172382119307 21.757876812831707 11 34.615241133352455 22.659463698599474 24.087055714264398 18.638239453783672 12 23.164370592789957 24.45650281613779 31.711684526247204 20.667442064825053 13 29.001120817865438 21.67895531816471 25.860551024570118 23.459372839399734 14 21.40471970434284 21.605919775305864 27.490296469589527 29.499064050761774 15 23.302856261740814 29.949007761166314 23.631184601686865 23.116951375406007 16 22.65569171539848 28.051700211065256 26.9841875148185 22.30842055871776 17 22.05893081270901 27.92096576034062 27.59645085396042 22.423652572989948 18 21.92881811745707 27.637776867711988 28.966178160300167 21.467226854530775 19 23.549661495455172 26.71771419381287 27.39928282549016 22.333341485241796 20 23.42249172339497 27.164424960922652 27.94883199041004 21.46425132527234 21 23.467411669126058 26.652504435196395 27.010263127352314 22.869820768325237 22 23.220381206691656 27.258396590752238 27.436718056022453 22.084504146533654 23 23.20250262486285 26.8834038616261 27.241164481595288 22.67292903191576 24 23.153737043641467 26.681124565910697 27.89027416123045 22.274864229217393 25 23.248100329779103 26.84163971011273 27.575145366429517 22.335114593678654 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 245.0 2 392.0 3 539.0 4 539.0 5 539.0 6 1487.5 7 2436.0 8 2436.0 9 2436.0 10 3538.5 11 4641.0 12 4641.0 13 4641.0 14 7646.0 15 10651.0 16 10651.0 17 10651.0 18 19252.5 19 27854.0 20 27854.0 21 27854.0 22 43501.0 23 59148.0 24 59148.0 25 59148.0 26 84276.0 27 109404.0 28 109404.0 29 109404.0 30 142372.0 31 175340.0 32 175340.0 33 175340.0 34 197605.5 35 219871.0 36 219871.0 37 219871.0 38 239398.0 39 258925.0 40 258925.0 41 258925.0 42 275594.5 43 292264.0 44 292264.0 45 292264.0 46 300329.5 47 308395.0 48 308395.0 49 308395.0 50 301398.0 51 294401.0 52 294401.0 53 294401.0 54 267317.5 55 240234.0 56 240234.0 57 240234.0 58 208646.0 59 177058.0 60 177058.0 61 177058.0 62 146820.5 63 116583.0 64 116583.0 65 116583.0 66 90895.0 67 65207.0 68 65207.0 69 65207.0 70 47669.0 71 30131.0 72 30131.0 73 30131.0 74 21375.5 75 12620.0 76 12620.0 77 12620.0 78 8584.5 79 4549.0 80 4549.0 81 4549.0 82 3008.0 83 1467.0 84 1467.0 85 1467.0 86 944.5 87 422.0 88 422.0 89 422.0 90 262.5 91 103.0 92 103.0 93 103.0 94 66.0 95 29.0 96 29.0 97 29.0 98 18.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.328122746136411 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.1450364846111376E-5 20 4.1450364846111376E-5 21 1.658014593844455E-4 22 0.001119159850845007 23 7.046562023838934E-4 24 8.290072969222275E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2412524.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.79028074146656 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22309694410313 60.578325907355556 2 11.404794262451203 16.603163523249805 3 2.867263928892374 6.2612683903186905 4 1.037489028672372 3.0207647065301364 5 0.5040980789924443 1.8346720345547007 6 0.2711196490365033 1.1840925220736978 7 0.16503831601342195 0.8409229749001104 8 0.10704045167354444 0.6233203622408547 9 0.0744829052339624 0.4879468424178155 >10 0.3150030030098274 3.8280076734431936 >50 0.01673057032197306 0.8411005506057171 >100 0.011309355576261415 1.6478554980773361 >500 0.0014970717408709904 0.7565609567029483 >1k 9.78854599788186E-4 1.2629467236230503 >5k 5.757968234048153E-5 0.22905133390634752 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5465 0.22652624388399867 No Hit GGTATCAACGCAGAGTACTTTTTTT 3353 0.13898307332901144 No Hit TATCAACGCAGAGTACTTTTTTTTT 3133 0.12986399306286694 No Hit GTCCTACAGTGGACATTTCTAAATT 2566 0.1063616361951218 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.1450364846111376E-5 0.0 4 0.0 0.0 0.0 4.1450364846111376E-5 0.0 5 0.0 0.0 0.0 4.1450364846111376E-5 0.0 6 0.0 0.0 0.0 4.1450364846111376E-5 0.0 7 0.0 0.0 0.0 4.1450364846111376E-5 0.0 8 0.0 0.0 0.0 4.1450364846111376E-5 0.0 9 0.0 0.0 0.0 4.1450364846111376E-5 0.0 10 0.0 0.0 0.0 4.1450364846111376E-5 0.0 11 0.0 0.0 0.0 4.1450364846111376E-5 0.0 12 0.0 0.0 0.0 4.1450364846111376E-5 0.0 13 0.0 0.0 0.0 4.1450364846111376E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 275 0.0 12.723538 18 GGTATCA 1250 0.0 12.099065 1 GCACCGT 150 1.9099389E-10 11.348021 6 CTAGTAC 125 1.9614163E-8 11.348021 3 ACCGTGC 125 1.9614163E-8 11.348021 8 CCAACGA 235 0.0 11.267538 19 TAGGACC 555 0.0 11.075396 4 AGGACCG 105 3.6633282E-6 10.807638 5 AATGCCG 395 0.0 10.7734375 18 GGACCGA 115 9.356918E-7 10.690165 6 CCCCGTA 80 3.9349718E-4 10.638769 15 CCCGTAC 80 3.9349718E-4 10.638769 16 GATATAC 970 0.0 10.496292 1 TAGACAG 345 0.0 10.416058 5 GTGCTAC 210 3.6379788E-12 10.355565 1 GTCCTAC 1155 0.0 10.269981 1 GTATCAA 2510 0.0 10.199913 1 ACCGCAT 75 0.0027507849 10.08713 8 GGACCGT 85 6.878688E-4 10.0129595 6 TATACAC 1255 0.0 9.946473 3 >>END_MODULE