FastQCFastQC Report
Thu 26 May 2016
SRR1511710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412524
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68870.2854686626951691No Hit
TATCAACGCAGAGTACTTTTTTTTT42680.17691015716320335No Hit
GGTATCAACGCAGAGTACTTTTTTT41230.17089985426051718No Hit
GTCCTACAGTGGACATTTCTAAATT28930.1199159054998002No Hit
CTGTAGGACGTGGAATATGGCAAGA26500.10984346684219513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAC400.005290819314.24569314
TAGGACC4250.012.2939714
GGTATCA15800.012.1371321
TACGACA550.003069566612.0907634
TCCAACG3200.011.87141118
AATCCCG5000.011.20684419
CTAGTAC1303.2692697E-810.9614073
GATATAC9650.010.4798631
CGGTTTC2450.010.46622413
TTAGGAC8200.010.3108523
CCGGTTT2600.010.22767812
GTCCTAA7500.010.1766721
CCAACGA2151.8189894E-1210.1599319
GGCGAGG6200.010.11005719
GCCGGTT2550.010.05578411
GTAGGAC12450.09.9958643
ACTGTTC10550.09.9922418
GTCCTAT2201.8189894E-129.9742961
TGTAGGA13650.09.9522622
TAGGACG13100.09.8624774
Produced by FastQC (version 0.11.5)