FastQCFastQC Report
Thu 26 May 2016
SRR1511709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68040.2748039437031568No Hit
GTCCTACAGTGGACATTTCTAAATT43160.17431714006802246No Hit
TATCAACGCAGAGTACTTTTTTTTT41440.1673703031607704No Hit
CTGTAGGACGTGGAATATGGCAAGA41140.1661586455606683No Hit
GGTATCAACGCAGAGTACTTTTTTT40040.1617159010269606No Hit
GTCCTAAAGTGTGTATTTCTCATTT37090.14980126795928994No Hit
CTTTAGGACGTGAAATATGGCGAGG31810.12847609419749292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC551.3990591E-414.3785391
GCGTATA450.00885049613.1803281
GGTATCA14850.012.381521
CGTAGGA655.747594E-412.1664572
GCACCGT1251.5170372E-912.1045026
GTACTAG1403.5106495E-1012.0035131
TCCAACG3100.011.59201318
TAGGACC4550.011.2232684
TACTGCG700.001549963310.80759057
CCAACGA2350.010.46266719
ACTGTCC5000.010.2131738
CACCTTT7000.010.13211614
ACCTTTT6950.010.06894215
TAGAAAT7450.010.0278484
GTCCTAC18050.010.02216151
GGCGAGG9100.09.97623819
GTCCTAA11050.09.8405161
GTATCAA28550.09.7640611
GTATAGA3250.09.7331661
TTATACT3500.09.7268324
Produced by FastQC (version 0.11.5)