##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511709_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2475947 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.31572848691834 31.0 30.0 34.0 23.0 34.0 2 26.661290003380525 31.0 26.0 34.0 10.0 34.0 3 28.313037395388513 31.0 27.0 34.0 16.0 34.0 4 32.79747466322987 35.0 32.0 37.0 26.0 37.0 5 33.23887344922973 35.0 35.0 37.0 27.0 37.0 6 33.48777498064377 37.0 35.0 37.0 28.0 37.0 7 33.529794862329446 37.0 35.0 37.0 28.0 37.0 8 33.54886918015612 37.0 35.0 37.0 28.0 37.0 9 35.13455417260547 39.0 35.0 39.0 28.0 39.0 10 35.00533533229912 39.0 35.0 39.0 28.0 39.0 11 35.01841356054875 39.0 35.0 39.0 28.0 39.0 12 34.92020628874528 39.0 35.0 39.0 27.0 39.0 13 34.91519891176992 39.0 35.0 39.0 27.0 39.0 14 36.053558497011444 40.0 36.0 41.0 27.0 41.0 15 35.98761645544109 40.0 36.0 41.0 27.0 41.0 16 35.92571488808121 40.0 36.0 41.0 27.0 41.0 17 35.89847440191571 40.0 36.0 41.0 27.0 41.0 18 35.833422120909695 40.0 36.0 41.0 27.0 41.0 19 35.85832087682006 40.0 36.0 41.0 27.0 41.0 20 35.78784400473839 40.0 36.0 41.0 27.0 41.0 21 35.701105879891614 40.0 36.0 41.0 27.0 41.0 22 35.597399298127144 40.0 36.0 41.0 27.0 41.0 23 35.483504695375146 40.0 36.0 41.0 26.0 41.0 24 35.36649976756368 40.0 36.0 41.0 26.0 41.0 25 35.25914771196637 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1918202847445798 1101 2 3.8103841790654727 1101 3 2.2474900950837053 1101 4 1.283899898945883 1101 5 0.8722700625649082 1101 6 0.7055289087430694 1101 7 0.6314428377116528 1101 8 0.47017611469334497 1101 9 0.49670140488792214 1101 10 0.6537457296610896 1101 11 0.4108482272307725 1101 12 0.5194864321014165 1101 13 0.47618744312288896 1101 14 0.6440331304080189 1101 15 0.6833446741759843 1101 16 0.6825415322456152 1101 17 0.6714427333787683 1101 18 0.6560768965500969 1101 19 0.7084603323720984 1101 20 0.8693155640051842 1101 21 0.8611597950118437 1101 22 0.7039547829544901 1101 23 0.6626059971175238 1101 24 0.6460643689292809 1101 25 0.6344561370226316 1102 1 1.4214691212304729 1102 2 3.997586589186618 1102 3 2.5541986775182863 1102 4 1.5844547040610024 1102 5 1.0620730683984547 1102 6 0.8724506778128926 1102 7 0.6714729642259698 1102 8 0.8868062062660229 1102 9 0.9543619340407545 1102 10 0.8232105685673332 1102 11 0.9910450509501842 1102 12 0.994986800693141 1102 13 0.9620284075122427 1102 14 1.1071722535432187 1102 15 1.1715535740681773 1102 16 1.1287686870157856 1102 17 1.2021325751613006 1102 18 1.0735762680266348 1102 19 1.1034623990424564 1102 20 1.0529545656968722 1102 21 1.0941432147760324 1102 22 0.9716959761689878 1102 23 1.0535535548244965 1102 24 0.8032670347209887 1102 25 0.7366691787778947 1103 1 1.4329489632971253 1103 2 4.01204787655772 1103 3 2.6619622226108923 1103 4 1.5516662242931218 1103 5 1.0981735324719182 1103 6 0.8312140827767465 1103 7 0.8766325873620531 1103 8 0.7458160729788474 1103 9 0.885658454726233 1103 10 0.8196908639917169 1103 11 0.9902220005945068 1103 12 1.0895860736721943 1103 13 1.0167140657464557 1103 14 1.1895218005976318 1103 15 0.9430126441078244 1103 16 0.9291820668388979 1103 17 1.11711810892821 1103 18 1.1907321489007145 1103 19 1.2518636948212887 1103 20 1.1200445036280726 1103 21 1.12130057936632 1103 22 1.296669392920144 1103 23 1.2820095832459089 1103 24 1.4116001619826264 1103 25 1.141879907694694 1104 1 1.4198228708450422 1104 2 3.8223468196856984 1104 3 2.4888566330603155 1104 4 1.5787558986036672 1104 5 1.1770591127122643 1104 6 0.9764399617680581 1104 7 0.8844241213265462 1104 8 0.8276457098680652 1104 9 0.8696194835263782 1104 10 0.7615072139967936 1104 11 0.8454055521535366 1104 12 0.9081171592161184 1104 13 0.8420699742332403 1104 14 1.05601194936132 1104 15 1.0435264716050128 1104 16 0.8452389996471865 1104 17 0.9811365756578638 1104 18 0.8311017266421246 1104 19 0.8007929986364033 1104 20 0.9157445416298202 1104 21 0.9216174958679773 1104 22 1.007804962669816 1104 23 1.0430425404443397 1104 24 0.9215521872324146 1104 25 0.8972682921813586 1105 1 1.4993707893951758 1105 2 3.8812549677051855 1105 3 2.6153420810811028 1105 4 1.7089762896291774 1105 5 1.248289030988488 1105 6 1.0002246500088319 1105 7 0.8287217397305184 1105 8 0.7944394555245893 1105 9 0.880287977287125 1105 10 0.7646980979176092 1105 11 0.8536297361449741 1105 12 0.9266843057712819 1105 13 0.7803940574946253 1105 14 0.7615461869354192 1105 15 0.7999777068244711 1105 16 0.8078014049007436 1105 17 0.9338867020178441 1105 18 0.9058471181056333 1105 19 1.1017712688638426 1105 20 0.9398668177786149 1105 21 1.0100949160030979 1105 22 0.9681826542128036 1105 23 0.963467117522228 1105 24 0.9054370742770459 1105 25 1.0073234572857714 1106 1 1.1990613977399676 1106 2 3.835721323558282 1106 3 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1107 16 1.0181512912603807 1107 17 1.0329748006210053 1107 18 1.0741393266556116 1107 19 1.0121878390550592 1107 20 1.2146016106234399 1107 21 1.061747656245494 1107 22 1.1429091115786818 1107 23 1.194302462175763 1107 24 1.1642525841953386 1107 25 1.2541338399583495 1108 1 1.2676551861139522 1108 2 3.8151422424557566 1108 3 2.4766779775577845 1108 4 1.3574884872908655 1108 5 1.0445494618728475 1108 6 0.7671145223787335 1108 7 0.5912029517799056 1108 8 0.6450619690903068 1108 9 0.5637695164493266 1108 10 0.5812949185773917 1108 11 0.6504183507959169 1108 12 0.6003535123246166 1108 13 0.6113408930725726 1108 14 0.6909804500568342 1108 15 0.7914061590479449 1108 16 0.5866775252095451 1108 17 0.6908983283715671 1108 18 0.7464870811941253 1108 19 0.6784830803711301 1108 20 0.7098833697571152 1108 21 0.6868704671776769 1108 22 0.8105351387801321 1108 23 0.7417812208527153 1108 24 0.8187914461415531 1108 25 0.9009024045434728 1109 1 1.1922205671619537 1109 2 3.1355202316186315 1109 3 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-2.0206718689170415 2315 12 -2.0927284248055997 2315 13 -2.051950735474975 2315 14 -2.179489870688357 2315 15 -2.1580897342315097 2315 16 -2.4034612466264704 2315 17 -2.320506840914696 2315 18 -2.282308949060557 2315 19 -2.2369516057271 2315 20 -2.288832570971131 2315 21 -2.3059458752395585 2315 22 -2.3865082947249547 2315 23 -2.2729783864333726 2315 24 -2.3127650979824352 2315 25 -2.326367810914199 2316 1 -0.7215804852671326 2316 2 1.6270703674024425 2316 3 0.24264927110664303 2316 4 -0.6696174158127235 2316 5 -0.9524309407313893 2316 6 -1.0890038730311744 2316 7 -1.5604644607417981 2316 8 -1.4586829668602093 2316 9 -1.8122992981923502 2316 10 -1.6805184409330423 2316 11 -1.803823114632067 2316 12 -1.9495671558215122 2316 13 -1.9270128101961035 2316 14 -2.233547463075773 2316 15 -2.12064900185333 2316 16 -2.18619255559215 2316 17 -2.072452061200984 2316 18 -2.344581162573178 2316 19 -2.2719448129449162 2316 20 -2.0644640289586107 2316 21 -2.4614102822819675 2316 22 -2.2841191147589655 2316 23 -2.3742124483472367 2316 24 -2.3613896325879296 2316 25 -2.358476142915876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89356.0 3 3.0 4 757.0 5 3986.0 6 4678.0 7 4129.0 8 3847.0 9 3765.0 10 3584.0 11 3528.0 12 3478.0 13 3406.0 14 3442.0 15 3668.0 16 3834.0 17 4152.0 18 4640.0 19 5823.0 20 7488.0 21 9769.0 22 11794.0 23 14128.0 24 16534.0 25 18678.0 26 21498.0 27 24214.0 28 28214.0 29 34252.0 30 42830.0 31 54339.0 32 70393.0 33 92708.0 34 126900.0 35 182515.0 36 290608.0 37 370369.0 38 908640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54091585966905 18.33500474767836 12.747324559047508 25.37675483360508 2 16.67753823731942 20.790200640641604 39.565846464713 22.96641465732598 3 19.043703277978082 24.168166766089904 29.402406432770977 27.385723523161037 4 12.395297637631177 16.654718376443437 38.133045658893344 32.81693832703204 5 13.399721399529149 37.47289420977105 35.656619467218 13.470764923481804 6 31.915424683969405 36.89832617580263 18.053819407281335 13.132429732946626 7 28.04203805654968 31.36424164168296 22.070423963033132 18.52329633873423 8 25.075132868353002 36.99974191693118 20.499590661674098 17.425534553041725 9 26.35104870984718 15.374157847482195 20.117958906228605 38.15683453644201 10 15.220277332269227 28.818750966801794 34.192492811841284 21.768478889087692 11 34.66116197156078 22.30536437169293 24.30427630316804 18.729197353578247 12 23.4841456622456 24.25375018124378 31.68767344373688 20.57443071277374 13 29.222111781875782 21.724778438310675 25.628133397039598 23.424976382773945 14 21.24496202867024 21.79093494327625 27.483302348555927 29.48080067949758 15 23.21572311523631 30.35109394506425 23.470211599844422 22.962971339855013 16 22.434527071863815 27.70810522196154 27.50838366087804 22.348984045296607 17 22.028621775829613 28.006496100280014 27.736498398390598 22.22838372549978 18 21.780595465088712 27.113302506071413 29.564647385424646 21.541454643415225 19 23.68012064883483 26.449526766738856 27.467763836529553 22.40258874789676 20 23.592144743001363 26.989349933580968 28.059566703164485 21.35893862025318 21 23.2897498686357 26.405900953133337 27.16714749434457 23.137201683886396 22 23.254220940907434 27.494487927561735 27.40175505840434 21.84953607312649 23 22.70560043878444 27.16145966911033 27.626375694637694 22.506564197467537 24 23.24627566444327 26.445538976027333 28.171223512638612 22.13696184689078 25 23.051745453355828 26.79560588332464 27.966794119583334 22.185854543736195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 354.0 1 354.0 2 541.5 3 729.0 4 729.0 5 729.0 6 1887.5 7 3046.0 8 3046.0 9 3046.0 10 4354.5 11 5663.0 12 5663.0 13 5663.0 14 8402.0 15 11141.0 16 11141.0 17 11141.0 18 19680.0 19 28219.0 20 28219.0 21 28219.0 22 44019.0 23 59819.0 24 59819.0 25 59819.0 26 86579.0 27 113339.0 28 113339.0 29 113339.0 30 147589.0 31 181839.0 32 181839.0 33 181839.0 34 203252.5 35 224666.0 36 224666.0 37 224666.0 38 242469.5 39 260273.0 40 260273.0 41 260273.0 42 278055.0 43 295837.0 44 295837.0 45 295837.0 46 306237.0 47 316637.0 48 316637.0 49 316637.0 50 310562.5 51 304488.0 52 304488.0 53 304488.0 54 275996.0 55 247504.0 56 247504.0 57 247504.0 58 215940.5 59 184377.0 60 184377.0 61 184377.0 62 152627.0 63 120877.0 64 120877.0 65 120877.0 66 93574.5 67 66272.0 68 66272.0 69 66272.0 70 48902.5 71 31533.0 72 31533.0 73 31533.0 74 22058.0 75 12583.0 76 12583.0 77 12583.0 78 8622.0 79 4661.0 80 4661.0 81 4661.0 82 3065.5 83 1470.0 84 1470.0 85 1470.0 86 949.0 87 428.0 88 428.0 89 428.0 90 285.5 91 143.0 92 143.0 93 143.0 94 90.5 95 38.0 96 38.0 97 38.0 98 24.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.337087990978805 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.038858667007008E-5 20 0.0 21 3.2310869336056065E-4 22 7.269945600612614E-4 23 6.462173867211213E-4 24 8.077717334014016E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2475947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.27831614852019 #Duplication Level Percentage of deduplicated Percentage of total 1 81.654994428208 57.38575513531256 2 12.498071754351905 17.566868759984672 3 3.2081196513440475 6.76383741298312 4 1.1300491929736993 3.17671817788743 5 0.5132419915264212 1.8034891470594974 6 0.2847201382023597 1.2005791131861479 7 0.15286926637185036 0.7520376242031273 8 0.11347929762253535 0.6380107163702845 9 0.07266746642844187 0.45962524614330136 >10 0.3339916438544876 4.019388125747513 >50 0.018931035736920898 0.9167990306341778 >100 0.015260333215812084 2.0755792658230883 >500 0.0023250323636273985 1.1553151713875827 >1k 0.0012206419908909346 1.8080547627599024 >5k 5.81258090900445E-5 0.27794231051757473 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6804 0.2748039437031568 No Hit GTCCTACAGTGGACATTTCTAAATT 4316 0.17431714006802246 No Hit TATCAACGCAGAGTACTTTTTTTTT 4144 0.1673703031607704 No Hit CTGTAGGACGTGGAATATGGCAAGA 4114 0.1661586455606683 No Hit GGTATCAACGCAGAGTACTTTTTTT 4004 0.1617159010269606 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3709 0.14980126795928994 No Hit CTTTAGGACGTGAAATATGGCGAGG 3181 0.12847609419749292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.0388586670070075E-5 0.0 9 0.0 0.0 0.0 1.615543466802803E-4 0.0 10 0.0 0.0 0.0 2.8272010669049057E-4 0.0 11 0.0 0.0 0.0 2.8272010669049057E-4 4.0388586670070075E-5 12 0.0 0.0 0.0 2.8272010669049057E-4 4.0388586670070075E-5 13 0.0 0.0 0.0 2.8272010669049057E-4 4.0388586670070075E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAC 55 1.3990591E-4 14.378539 1 GCGTATA 45 0.008850496 13.180328 1 GGTATCA 1485 0.0 12.38152 1 CGTAGGA 65 5.747594E-4 12.166457 2 GCACCGT 125 1.5170372E-9 12.104502 6 GTACTAG 140 3.5106495E-10 12.003513 1 TCCAACG 310 0.0 11.592013 18 TAGGACC 455 0.0 11.223268 4 TACTGCG 70 0.0015499633 10.8075905 7 CCAACGA 235 0.0 10.462667 19 ACTGTCC 500 0.0 10.213173 8 CACCTTT 700 0.0 10.132116 14 ACCTTTT 695 0.0 10.068942 15 TAGAAAT 745 0.0 10.027848 4 GTCCTAC 1805 0.0 10.0221615 1 GGCGAGG 910 0.0 9.976238 19 GTCCTAA 1105 0.0 9.840516 1 GTATCAA 2855 0.0 9.764061 1 GTATAGA 325 0.0 9.733166 1 TTATACT 350 0.0 9.726832 4 >>END_MODULE