FastQCFastQC Report
Thu 26 May 2016
SRR1511709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86050.347543788295953No Hit
TATCAACGCAGAGTACTTTTTTTTT53270.21515000119146332No Hit
GGTATCAACGCAGAGTACTTTTTTT51730.20893015884427252No Hit
GTCCTACAGTGGACATTTCTAAATT45680.18449506390888012No Hit
CTGTAGGACGTGGAATATGGCAAGA43100.17407480854800203No Hit
GTCCTAAAGTGTGTATTTCTCATTT41020.16567398252062746No Hit
CTTTAGGACGTGAAATATGGCGAGG34330.1386540180383506No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26950.10884724107583885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.012.3293831
CGAAATC1201.0068106E-811.87147813
GTTAGAC855.3230462E-511.1769933
ACAACGC600.00589031611.0800478
CGTGCGG600.00589031611.08004710
GGCGAGG8950.010.50526319
GTCCTAA12550.010.4118091
TCCAACG3150.010.25092818
ACGAAAT1409.737414E-810.17555312
ACGGTAC750.00264749210.1338073
TATCGCG750.00265504410.1303297
GTATCAA33200.010.0262071
ATTTAGA6100.010.0068981
AGGACGT22650.09.7730845
TGTAGGA23150.09.6030412
TAGGACC4650.09.60264
GTCCTAC21100.09.5830271
CTAAAGT13900.09.5687934
GACGTGA11650.09.5379437
GTACTAA1803.9071892E-99.5378241
Produced by FastQC (version 0.11.5)