##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511709_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2475947 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.357436972600787 33.0 31.0 34.0 28.0 34.0 2 31.53393671189246 33.0 31.0 34.0 28.0 34.0 3 31.650052283025445 34.0 31.0 34.0 28.0 34.0 4 35.18712032204243 37.0 35.0 37.0 32.0 37.0 5 34.872423359627646 37.0 35.0 37.0 32.0 37.0 6 34.83796704856768 37.0 35.0 37.0 32.0 37.0 7 34.688890755739116 37.0 35.0 37.0 32.0 37.0 8 34.613932769966404 37.0 35.0 37.0 32.0 37.0 9 36.18377331986509 39.0 37.0 39.0 32.0 39.0 10 36.01216908116369 39.0 37.0 39.0 31.0 39.0 11 36.0122854002933 39.0 37.0 39.0 31.0 39.0 12 35.86403505406214 39.0 37.0 39.0 30.0 39.0 13 35.851218947739994 39.0 37.0 39.0 30.0 39.0 14 36.963573533682265 40.0 37.0 41.0 31.0 41.0 15 36.892490025028806 40.0 37.0 41.0 31.0 41.0 16 36.74540367786548 40.0 37.0 41.0 30.0 41.0 17 36.72729060840155 40.0 37.0 41.0 30.0 41.0 18 36.670525661494366 40.0 37.0 41.0 30.0 41.0 19 36.68372061275948 40.0 37.0 41.0 30.0 41.0 20 36.79323830437404 40.0 37.0 41.0 31.0 41.0 21 36.74159907300116 40.0 37.0 41.0 30.0 41.0 22 36.651864115023464 40.0 37.0 41.0 30.0 41.0 23 36.562753160709825 40.0 37.0 41.0 30.0 41.0 24 36.42847201495024 40.0 37.0 41.0 30.0 41.0 25 35.93864408244603 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11566727511982577 1101 2 0.21086595207821546 1101 3 0.15668305068158617 1101 4 0.14601417284515605 1101 5 0.3506790505868693 1101 6 0.1668016485837711 1101 7 0.3112139167417709 1101 8 0.1191351467678814 1101 9 0.2604767554647651 1101 10 0.3511243617573143 1101 11 0.260472658061218 1101 12 0.34884169194695147 1101 13 0.4296272496459892 1101 14 0.06190636489009904 1101 15 0.1577314457220922 1101 16 0.11445996350472853 1101 17 -0.002494685728635204 1101 18 0.19871091723867096 1101 19 0.09672161118188427 1101 20 -0.06979981821768888 1101 21 -0.015319123700265891 1101 22 0.07924590872085702 1101 23 0.024392075436878713 1101 24 -0.21356804373355942 1101 25 0.10633277448491896 1102 1 0.48457453914655346 1102 2 0.48742287925897543 1102 3 0.5200607996727484 1102 4 0.4836944738546265 1102 5 0.44097805730674366 1102 6 0.463502859183869 1102 7 0.654279918096826 1102 8 0.42144342580673566 1102 9 0.5163573914026713 1102 10 0.4762740355642947 1102 11 0.6307002474713457 1102 12 0.6677981725564663 1102 13 0.6487935931734725 1102 14 0.8649935869016332 1102 15 0.6884379060910248 1102 16 0.8507877672520081 1102 17 0.8775708937699989 1102 18 0.7719588281971426 1102 19 1.0159303534105675 1102 20 0.8496922922149466 1102 21 0.6867590518346134 1102 22 0.760177146123759 1102 23 0.9161946381081236 1102 24 0.8179908648940426 1102 25 0.8979678729393896 1103 1 0.2332490859948919 1103 2 0.35504200930670393 1103 3 0.3171757959222461 1103 4 0.36494936805741673 1103 5 0.4269025361514238 1103 6 0.4560105044438103 1103 7 0.27526708395156874 1103 8 0.3609630314679322 1103 9 0.5593826887641313 1103 10 0.6061960580343495 1103 11 0.34893924118203756 1103 12 0.5273005506238491 1103 13 0.5293931793815077 1103 14 0.6516920710382337 1103 15 0.7124155844048659 1103 16 0.7855891553509551 1103 17 0.9310122911009557 1103 18 0.8040763385213339 1103 19 0.8264956492883044 1103 20 0.6951826192095183 1103 21 0.8997539330136775 1103 22 0.9128853403586916 1103 23 0.8661188037238219 1103 24 0.8856347235453441 1103 25 0.9388809354120795 1104 1 0.3206248597068644 1104 2 0.3929372774425346 1104 3 0.40996985745369585 1104 4 0.47670822130676527 1104 5 0.44429098103301357 1104 6 0.5578583147158866 1104 7 0.555777601492963 1104 8 0.5594801791174575 1104 9 0.7199418203007539 1104 10 0.7351880549220553 1104 11 0.768762229138737 1104 12 0.7874156225702862 1104 13 0.642586216081618 1104 14 0.9695443019052945 1104 15 0.9448813485203118 1104 16 1.0211456495116664 1104 17 0.9614209412233379 1104 18 0.9558427136844259 1104 19 0.8491466885712882 1104 20 0.9542396033927147 1104 21 1.0284463083108975 1104 22 0.915017987229156 1104 23 1.0438867640576888 1104 24 0.9889988779006629 1104 25 0.9821350768766095 1105 1 0.42351626622957994 1105 2 0.4619670343472144 1105 3 0.48222874065939436 1105 4 0.4555213775144509 1105 5 0.4178268771584186 1105 6 0.4878044909205741 1105 7 0.5353056829857792 1105 8 0.46339026468807987 1105 9 0.5768092569516838 1105 10 0.6545900721981894 1105 11 0.6872820342646122 1105 12 0.4931585919535877 1105 13 0.5517735617345281 1105 14 0.7391669287348321 1105 15 0.7099169619723611 1105 16 0.7015683954765777 1105 17 0.7630074160049318 1105 18 0.6739837496987633 1105 19 0.5237986028473145 1105 20 0.7516778160455928 1105 21 0.6957694377734072 1105 22 0.5772963250683745 1105 23 0.8549720432468462 1105 24 0.8672187497340573 1105 25 1.0145535005106296 1106 1 0.11345317508994412 1106 2 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0.8623869798220554 1107 15 0.7753024963969679 1107 16 0.950248333460614 1107 17 0.8972208524265355 1107 18 0.7931384950869287 1107 19 0.8303710353808782 1107 20 0.8941522678026175 1107 21 0.9723439634383482 1107 22 0.9146953963107833 1107 23 0.88956353994692 1107 24 0.9968328541053637 1107 25 0.9452982662929301 1108 1 0.038604230827068875 1108 2 0.1813999546582572 1108 3 0.10617487117425384 1108 4 0.10425655883067009 1108 5 0.1257372223027602 1108 6 0.10927343431112746 1108 7 0.09887706071974378 1108 8 0.21378723519568155 1108 9 0.14153271690069857 1108 10 0.25166176389733863 1108 11 0.2170528063665529 1108 12 0.25833208620912984 1108 13 0.1804445966837065 1108 14 0.23210244894412568 1108 15 0.38436871493592406 1108 16 0.47153393468506266 1108 17 0.28281649852186064 1108 18 0.2926312135927702 1108 19 0.17322359601931936 1108 20 0.26523757128246217 1108 21 0.3454821008959428 1108 22 0.35062870709827365 1108 23 0.19924302869085153 1108 24 0.24790128306678128 1108 25 0.22817341926183587 1109 1 -0.05906043942479755 1109 2 0.017776114016868405 1109 3 0.004235750987881204 1109 4 0.03860844869560509 1109 5 -0.014027989481881775 1109 6 -0.15649447365256464 1109 7 -0.15664704911898042 1109 8 -0.020259320641009992 1109 9 -0.14086130504853855 1109 10 0.03963287136566862 1109 11 0.10513274448448584 1109 12 0.06259592483177556 1109 13 0.013372564963653133 1109 14 0.04315024805129042 1109 15 0.10971383329572859 1109 16 0.1618908403359427 1109 17 -0.009465777902164518 1109 18 -0.009062293546087119 1109 19 -0.2173443388608689 1109 20 -0.020484617455892362 1109 21 -0.09921824439226157 1109 22 -0.2019173445393463 1109 23 9.159925887729514E-4 1109 24 0.09397999894275699 1109 25 -0.03261590705250228 1110 1 -0.2073569328889171 1110 2 -0.16886936493272842 1110 3 -0.13312137080002273 1110 4 -0.04970614978229548 1110 5 -0.038232843722433074 1110 6 0.003620196650750529 1110 7 -0.12658740585191453 1110 8 -0.057455656762073204 1110 9 -0.056362999361738275 1110 10 0.008010125396850754 1110 11 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-0.7677924398022924 2314 24 -0.9001796620868632 2314 25 -0.8524601790215343 2315 1 -0.6731269987491153 2315 2 -0.7443469820862063 2315 3 -0.7482656647349586 2315 4 -0.7320384309622625 2315 5 -0.9097957143514748 2315 6 -0.9318742583998798 2315 7 -0.9230254933302078 2315 8 -1.0610047859109386 2315 9 -1.022289887507867 2315 10 -1.144215311319968 2315 11 -1.091317498174405 2315 12 -1.0428725802918493 2315 13 -1.0550365237741346 2315 14 -1.195346733943019 2315 15 -1.270091068514553 2315 16 -1.2524999708866602 2315 17 -1.3180673133348932 2315 18 -1.2760204414154757 2315 19 -1.3854560440062755 2315 20 -1.253809352407842 2315 21 -1.3760489569206271 2315 22 -1.3574967452256175 2315 23 -1.4715204366460881 2315 24 -1.3809516622475115 2315 25 -1.379914739282377 2316 1 -0.9870288163691505 2316 2 -1.1010440176300555 2316 3 -1.0564802327708165 2316 4 -0.997086898883289 2316 5 -1.2025977011672637 2316 6 -1.2863696786471976 2316 7 -1.3349702782838406 2316 8 -2.037746448361432 2316 9 -1.7262253478482705 2316 10 -1.743815336165781 2316 11 -1.6508829512362482 2316 12 -1.6701462776517673 2316 13 -1.805779482666516 2316 14 -1.7220008255125876 2316 15 -1.852206222497557 2316 16 -1.8239673127082767 2316 17 -1.8883144495626922 2316 18 -1.7797611700243152 2316 19 -1.6618578815348215 2316 20 -1.7199976486485298 2316 21 -1.750714825831345 2316 22 -1.770366346636095 2316 23 -1.796764262752987 2316 24 -1.848832659286721 2316 25 -1.8949216647879297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27707.0 3 1.0 4 471.0 5 4664.0 6 6025.0 7 4904.0 8 4006.0 9 3584.0 10 3300.0 11 3236.0 12 3081.0 13 2925.0 14 2872.0 15 2933.0 16 3020.0 17 3181.0 18 3431.0 19 3933.0 20 4700.0 21 6196.0 22 7892.0 23 9855.0 24 12242.0 25 14950.0 26 17639.0 27 19914.0 28 23267.0 29 28997.0 30 36969.0 31 48065.0 32 62518.0 33 81898.0 34 108996.0 35 148902.0 36 221811.0 37 409805.0 38 1128057.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.675532590260545 18.89075353454469 13.224083217012709 25.209630658182057 2 15.003673341957644 20.33884408672722 40.68653327393518 23.970949297379953 3 18.980656694186106 24.492648671397248 29.806696185338378 26.71999844907827 4 12.728584254832596 17.045881838343067 37.873508600951475 32.352025305872864 5 13.348099281473125 37.55971562755549 35.60408942960667 13.488095661364715 6 31.88270992876665 37.135043682275914 17.927322353830675 13.054924035126762 7 28.068896466685274 31.401076032726067 22.077613131460406 18.452414369128256 8 25.1416528705986 37.09909784013955 20.366227548489526 17.39302174077232 9 26.32225164755142 15.326256983691492 20.035929686701696 38.315561682055396 10 15.123869775887771 28.997874348683556 34.11256379882121 21.765692076607458 11 34.6966231506571 22.270226301289973 24.302297262421206 18.73085328563172 12 23.36407039407548 24.40116852258954 31.73549353035424 20.499267552980736 13 29.23846915947716 21.64230494433039 25.6688854809897 23.45034041520275 14 21.148352529355435 21.765813242367464 27.53382847048018 29.552005757796916 15 23.13413817016277 30.398227425708225 23.47526017317818 22.992374230950823 16 22.51288093000375 27.752048004258572 27.37732269713366 22.357748368604014 17 21.989808344039673 28.064090224871535 27.68128720041261 22.26481423067618 18 21.77259852492804 27.1142718321515 29.580843208679347 21.532286434241122 19 23.63600674812506 26.489581562125526 27.55515364424198 22.319258045507436 20 23.586369175107542 26.972911778806253 28.07281415959227 21.367904886493935 21 23.24791281881236 26.373464375449068 27.243232589389027 23.135390216349542 22 23.20918016419576 27.425910166897754 27.50907026685143 21.855839402055054 23 22.681341725004614 27.17703569583679 27.68819364873319 22.45342893042541 24 23.21588466958299 26.408440891505354 28.295395660731025 22.08027877818063 25 22.98779591355358 26.819586637866273 28.013338223153518 22.179279225426633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 302.0 1 302.0 2 515.5 3 729.0 4 729.0 5 729.0 6 1951.0 7 3173.0 8 3173.0 9 3173.0 10 4295.0 11 5417.0 12 5417.0 13 5417.0 14 8152.5 15 10888.0 16 10888.0 17 10888.0 18 19710.5 19 28533.0 20 28533.0 21 28533.0 22 44813.0 23 61093.0 24 61093.0 25 61093.0 26 88592.5 27 116092.0 28 116092.0 29 116092.0 30 149973.5 31 183855.0 32 183855.0 33 183855.0 34 203617.0 35 223379.0 36 223379.0 37 223379.0 38 241657.0 39 259935.0 40 259935.0 41 259935.0 42 278066.0 43 296197.0 44 296197.0 45 296197.0 46 306717.5 47 317238.0 48 317238.0 49 317238.0 50 310295.5 51 303353.0 52 303353.0 53 303353.0 54 274943.0 55 246533.0 56 246533.0 57 246533.0 58 215369.0 59 184205.0 60 184205.0 61 184205.0 62 152126.0 63 120047.0 64 120047.0 65 120047.0 66 92986.5 67 65926.0 68 65926.0 69 65926.0 70 48302.0 71 30678.0 72 30678.0 73 30678.0 74 21418.0 75 12158.0 76 12158.0 77 12158.0 78 8293.5 79 4429.0 80 4429.0 81 4429.0 82 2883.5 83 1338.0 84 1338.0 85 1338.0 86 849.0 87 360.0 88 360.0 89 360.0 90 220.0 91 80.0 92 80.0 93 80.0 94 43.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4353081871300153 2 0.0 3 0.0 4 0.0 5 0.03566312202967188 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0022213722668538542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2475947.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.53926012996448 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97995833036661 54.0178721085835 2 13.337044177405561 18.015481921252444 3 3.6198478350629197 7.334455336896097 4 1.3179917590294954 3.5606475304897014 5 0.6157785105522544 2.0794612503315393 6 0.30836834442003097 1.249618189777857 7 0.18626431992895 0.8806108049628596 8 0.12357899027431606 0.6677146856588305 9 0.08598197255493804 0.5226442929787883 >10 0.38044045195496895 4.4098020106421165 >50 0.023562510289827428 1.0962708029001547 >100 0.01711970824830926 2.3327285760314282 >500 0.0022705502449196515 1.0914979390331903 >1k 0.0016132857003247913 1.9702007942184134 >5k 1.792539667027546E-4 0.7709937562430672 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8605 0.347543788295953 No Hit TATCAACGCAGAGTACTTTTTTTTT 5327 0.21515000119146332 No Hit GGTATCAACGCAGAGTACTTTTTTT 5173 0.20893015884427252 No Hit GTCCTACAGTGGACATTTCTAAATT 4568 0.18449506390888012 No Hit CTGTAGGACGTGGAATATGGCAAGA 4310 0.17407480854800203 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4102 0.16567398252062746 No Hit CTTTAGGACGTGAAATATGGCGAGG 3433 0.1386540180383506 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2695 0.10884724107583885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 8.077717334014015E-5 0.0 7 0.0 0.0 0.0 1.2116576001021024E-4 0.0 8 0.0 0.0 0.0 1.2116576001021024E-4 0.0 9 0.0 0.0 0.0 2.019429333503504E-4 0.0 10 0.0 0.0 0.0 2.4233152002042048E-4 0.0 11 0.0 0.0 0.0 2.4233152002042048E-4 0.0 12 0.0 0.0 0.0 2.8272010669049057E-4 0.0 13 0.0 0.0 0.0 2.8272010669049057E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1640 0.0 12.329383 1 CGAAATC 120 1.0068106E-8 11.871478 13 GTTAGAC 85 5.3230462E-5 11.176993 3 ACAACGC 60 0.005890316 11.080047 8 CGTGCGG 60 0.005890316 11.080047 10 GGCGAGG 895 0.0 10.505263 19 GTCCTAA 1255 0.0 10.411809 1 TCCAACG 315 0.0 10.250928 18 ACGAAAT 140 9.737414E-8 10.175553 12 ACGGTAC 75 0.002647492 10.133807 3 TATCGCG 75 0.002655044 10.130329 7 GTATCAA 3320 0.0 10.026207 1 ATTTAGA 610 0.0 10.006898 1 AGGACGT 2265 0.0 9.773084 5 TGTAGGA 2315 0.0 9.603041 2 TAGGACC 465 0.0 9.6026 4 GTCCTAC 2110 0.0 9.583027 1 CTAAAGT 1390 0.0 9.568793 4 GACGTGA 1165 0.0 9.537943 7 GTACTAA 180 3.9071892E-9 9.537824 1 >>END_MODULE