FastQCFastQC Report
Thu 26 May 2016
SRR1511708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611193
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60920.2333033215085978No Hit
GGTATCAACGCAGAGTACTTTTTTT34930.1337702728216566No Hit
TATCAACGCAGAGTACTTTTTTTTT34840.13342560278003196No Hit
GTCCTACAGTGGACATTTCTAAATT30490.11676655076817379No Hit
CTGTAGGACGTGGAATATGGCAAGA28780.11021781997730538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGCA550.00317615712.035836
TCCAACG3000.011.97851818
TAGGACC5000.011.7263374
CCAACGA2350.011.6719
GTATCAA25450.011.1855861
AGGACGT15100.010.8345255
TTAGGAC9150.010.7486263
TAGGACG15800.010.713634
GTATACG650.00793356210.644831
GGACGTG15400.010.6234646
GTCCTAC15950.010.5971591
GTAGGAC15600.010.4872653
GGTATCA11900.010.3828621
TGTAGGA15900.010.25748352
CGGTTTC2053.6379788E-1210.1486813
GTAGAAC7550.010.1456253
GGCGAGG7300.010.104444519
GACGTGA8000.010.047777
CGAAATC951.7223225E-49.95444713
TAGAACA9150.09.8184574
Produced by FastQC (version 0.11.5)