##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511708_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2611193 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.224376750397234 31.0 30.0 34.0 23.0 34.0 2 26.572702592263383 31.0 25.0 34.0 10.0 34.0 3 28.226031549563743 31.0 27.0 34.0 16.0 34.0 4 32.7039426040128 35.0 32.0 37.0 26.0 37.0 5 33.14854359673912 35.0 35.0 37.0 27.0 37.0 6 33.392961761156684 37.0 35.0 37.0 28.0 37.0 7 33.430076214205535 37.0 35.0 37.0 28.0 37.0 8 33.45118227568778 37.0 35.0 37.0 28.0 37.0 9 35.024820072664106 39.0 35.0 39.0 28.0 39.0 10 34.90780842319966 39.0 35.0 39.0 28.0 39.0 11 34.92040343245405 39.0 35.0 39.0 27.0 39.0 12 34.82108867479347 39.0 35.0 39.0 27.0 39.0 13 34.81248034902055 39.0 35.0 39.0 27.0 39.0 14 35.9415274933718 40.0 36.0 41.0 27.0 41.0 15 35.874019653085774 40.0 36.0 41.0 27.0 41.0 16 35.81149957126876 40.0 36.0 41.0 27.0 41.0 17 35.78498831760042 40.0 36.0 41.0 27.0 41.0 18 35.722490830819474 40.0 36.0 41.0 27.0 41.0 19 35.7442605736152 40.0 36.0 41.0 27.0 41.0 20 35.667885139091595 40.0 36.0 41.0 27.0 41.0 21 35.58255938952042 40.0 36.0 41.0 27.0 41.0 22 35.47307265299808 40.0 36.0 41.0 26.0 41.0 23 35.36071596392913 40.0 36.0 41.0 26.0 41.0 24 35.24345576906801 40.0 36.0 41.0 25.0 41.0 25 35.13425166197979 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2813722432149923 1101 2 3.7754707503939606 1101 3 2.3224929745196725 1101 4 1.3286252991031162 1101 5 0.9910557269055431 1101 6 0.9007224926461035 1101 7 0.7095378078373002 1101 8 0.3678023758739357 1101 9 0.4404033723874079 1101 10 0.604550920292688 1101 11 0.44329272497720495 1101 12 0.5697028113243121 1101 13 0.5531272963624829 1101 14 0.5857328899272574 1101 15 0.5553364701960888 1101 16 0.7541950841058593 1101 17 0.7284832101601069 1101 18 0.6744364290931841 1101 19 0.7695553464263725 1101 20 0.8045825323306133 1101 21 0.7663508273928628 1101 22 0.6911281428399505 1101 23 0.6887850494519938 1101 24 0.7573563229809537 1101 25 0.6776132854026997 1102 1 1.4674479904662832 1102 2 4.162191705364354 1102 3 2.646669935499112 1102 4 1.6539480716297277 1102 5 1.2293777820349305 1102 6 0.9638815591751353 1102 7 0.8738262689865266 1102 8 0.8645133682434363 1102 9 0.9566606278820231 1102 10 0.8955431998392243 1102 11 0.9627108011382575 1102 12 0.9554173861821269 1102 13 1.0794539752181578 1102 14 1.1388101641776487 1102 15 1.1533354042202788 1102 16 1.1762281743337368 1102 17 1.0643802827746427 1102 18 1.069849359784513 1102 19 1.0263453199568886 1102 20 1.0376826553451224 1102 21 1.0329336103093638 1102 22 1.0535025702152865 1102 23 1.0533296596399353 1102 24 0.7770487642229753 1102 25 0.7793566281139235 1103 1 1.431570715449034 1103 2 4.0094052478389735 1103 3 2.623935275752604 1103 4 1.5611792986283959 1103 5 1.1529169620462483 1103 6 0.9218110072318311 1103 7 0.8264304148772013 1103 8 0.5658144439411785 1103 9 0.7828754601301995 1103 10 0.7066674598445672 1103 11 0.7142871910428568 1103 12 0.8584284090922267 1103 13 0.8284353052657423 1103 14 0.9084234389466914 1103 15 0.7452096091923366 1103 16 0.7990898169408851 1103 17 0.9429343624037614 1103 18 1.0314808125304893 1103 19 0.8739910030567231 1103 20 0.9181972498790216 1103 21 0.7474146057249769 1103 22 0.9133039628161512 1103 23 0.7931673372792787 1103 24 1.0884862338438595 1103 25 0.5569449288483383 1104 1 1.4745900841811128 1104 2 3.987458747996868 1104 3 2.5293755064579493 1104 4 1.669986347216465 1104 5 1.1880089645669827 1104 6 1.158462747051182 1104 7 1.0174634556554594 1104 8 0.986431925741698 1104 9 0.9765937336239858 1104 10 0.9309047368982633 1104 11 0.9731473113201403 1104 12 0.8375068258169733 1104 13 0.9075124699716071 1104 14 0.9643695548363382 1104 15 1.1307114392266016 1104 16 0.9321049983653751 1104 17 1.0389742962415198 1104 18 0.9530421774536535 1104 19 1.13568033822078 1104 20 1.167441169588443 1104 21 1.147955269211515 1104 22 1.2333154674785334 1104 23 1.1994533762949686 1104 24 1.1892626246629376 1104 25 1.1073522523241124 1105 1 1.4935503832731563 1105 2 3.9104385909339854 1105 3 2.4624269574104147 1105 4 1.6792132971501985 1105 5 1.1759503330317571 1105 6 0.9607524232326128 1105 7 0.968674066301709 1105 8 0.9030461721761327 1105 9 0.8763009715722774 1105 10 0.8289889257693517 1105 11 0.8392680163228121 1105 12 0.9392139450779524 1105 13 0.8455586789008507 1105 14 0.7447396614571176 1105 15 0.8336845565490165 1105 16 0.9092705473931275 1105 17 0.9220534409015784 1105 18 0.9125480425852928 1105 19 0.9310584562355402 1105 20 0.9570194339842217 1105 21 1.023478977003336 1105 22 1.060813340997413 1105 23 1.0855086054603973 1105 24 0.9839414765609504 1105 25 1.0222979528760092 1106 1 1.0389149016993606 1106 2 3.5015503943877846 1106 3 2.0596370636355665 1106 4 1.1573140033397422 1106 5 0.5756306190681997 1106 6 0.6670799408873549 1106 7 0.4376276892321016 1106 8 0.3852052428544255 1106 9 0.4482076833401294 1106 10 0.3380418009696342 1106 11 0.3802336860160054 1106 12 0.38302588502216395 1106 13 0.47629795253446616 1106 14 0.6056915337495639 1106 15 0.6294948942144316 1106 16 0.5828552011228751 1106 17 0.4607099004588733 1106 18 0.5982165489053983 1106 19 0.6398368670123489 1106 20 0.600328436417314 1106 21 0.5875842229828052 1106 22 0.41904133933579146 1106 23 0.5054651362300859 1106 24 0.5810914973312293 1106 25 0.39734616959905367 1107 1 1.5189009121762425 1107 2 3.9480160854359774 1107 3 2.649435246709956 1107 4 1.7114982778947407 1107 5 1.3054465068184697 1107 6 1.0972964918556727 1107 7 0.9788854285132516 1107 8 0.9183689373988884 1107 9 1.003398920642347 1107 10 1.0440086787820562 1107 11 0.9674934295438931 1107 12 1.058844558764747 1107 13 0.9831894120013303 1107 14 1.025273739135713 1107 15 1.1183836838340468 1107 16 1.1916187178760538 1107 17 1.1521675804762666 1107 18 1.2932955722148165 1107 19 1.2287343675111728 1107 20 1.3308056436945463 1107 21 1.3237899501253096 1107 22 1.322916414904526 1107 23 1.423133367876936 1107 24 1.5333160474180403 1107 25 1.4322874046677114 1108 1 1.5286388411772975 1108 2 4.049420880641808 1108 3 2.5555725223528327 1108 4 1.5624259422801003 1108 5 1.2540271805436163 1108 6 1.0539878026520029 1108 7 0.7742776476539817 1108 8 0.7618559432427716 1108 9 0.6998099109194413 1108 10 0.6507946645402001 1108 11 0.8569455636391368 1108 12 0.8142277446981083 1108 13 0.8365683433127984 1108 14 0.852288287576286 1108 15 0.8739504448235493 1108 16 0.9004822943782642 1108 17 0.96444213176202 1108 18 0.9415216315156627 1108 19 0.8313807592284235 1108 20 0.8870347772907081 1108 21 0.8930547907131441 1108 22 1.0238210032949766 1108 23 0.9574691806472799 1108 24 1.079026940649193 1108 25 1.010493430342862 1109 1 1.1123003259436999 1109 2 3.102265703639109 1109 3 2.0808254322939987 1109 4 1.3139144348723661 1109 5 0.9694554407041238 1109 6 0.7038374206489664 1109 7 0.5952010937006023 1109 8 0.5865251987678235 1109 9 0.6245815465179305 1109 10 0.6115859960048127 1109 11 0.5165979618674683 1109 12 0.5648162101265513 1109 13 0.5142205635264645 1109 14 0.6662550242226288 1109 15 0.6079223805368841 1109 16 0.6510683074166081 1109 17 0.543718166010656 1109 18 0.5100062838093038 1109 19 0.5188680735193785 1109 20 0.6035093424711278 1109 21 0.588713324532975 1109 22 0.657006053582684 1109 23 0.6312384017955921 1109 24 0.5888097411142255 1109 25 0.6926023646452109 1110 1 0.9394536125558162 1110 2 3.383347100578728 1110 3 2.0300724667079244 1110 4 1.1933924463054595 1110 5 0.5685967403500314 1110 6 0.5578152966595837 1110 7 0.2943089669806156 1110 8 0.32125264510978724 1110 9 0.2966752666259822 1110 10 0.3258620174752309 1110 11 0.17944127446187252 1110 12 0.19465714825813762 1110 13 0.24131104069545728 1110 14 0.33258165769740344 1110 15 0.2039522864722656 1110 16 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-1.733981922382263 2315 15 -1.7079679357095756 2315 16 -1.9469082117753445 2315 17 -1.721749969679074 2315 18 -1.7085888860449572 2315 19 -1.777121267562329 2315 20 -1.9500437825930348 2315 21 -1.9003349264875453 2315 22 -1.8822453038522085 2315 23 -1.6512982916398116 2315 24 -1.6532593536360594 2315 25 -1.630724723611408 2316 1 -0.599540194857461 2316 2 1.7134183460372725 2316 3 0.37213448863436227 2316 4 -0.5784127649994204 2316 5 -0.7663528585796513 2316 6 -0.9549673910680667 2316 7 -1.5795454229486836 2316 8 -1.3911036874786262 2316 9 -1.499955825802843 2316 10 -1.5661464098737525 2316 11 -1.4394148034310703 2316 12 -1.6090411084979834 2316 13 -1.5561243167625989 2316 14 -1.8020496065296356 2316 15 -1.8432055152679752 2316 16 -2.037692335688199 2316 17 -1.8376800943453446 2316 18 -2.164964596134695 2316 19 -1.9331297975943187 2316 20 -1.8464142442848228 2316 21 -2.05335946917981 2316 22 -1.8998593983734438 2316 23 -2.07848691266976 2316 24 -2.098179227995111 2316 25 -2.0428057955274213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97667.0 3 2.0 4 876.0 5 4235.0 6 5131.0 7 4589.0 8 4142.0 9 4098.0 10 3943.0 11 3872.0 12 3768.0 13 3583.0 14 3898.0 15 3862.0 16 4179.0 17 4509.0 18 5133.0 19 6377.0 20 8185.0 21 10485.0 22 12692.0 23 15446.0 24 17895.0 25 20371.0 26 23255.0 27 26385.0 28 30503.0 29 36833.0 30 46358.0 31 58344.0 32 75101.0 33 99293.0 34 135060.0 35 192801.0 36 305352.0 37 388153.0 38 944817.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58505863028891 18.438660030108846 12.786454314177467 25.189827025424776 2 16.67123665480071 20.849713571824 39.433380180715346 23.045669592659944 3 19.044628259956273 24.153365913588157 29.347543440871664 27.454462385583906 4 12.342481003893623 16.596398657625077 38.24692391562018 32.814196422861116 5 13.444659203666676 37.4668973147523 35.67105150787398 13.417391973707037 6 31.9442492378005 36.91864216854135 18.122750788624202 13.014357805033944 7 28.260530722930095 31.33387689075453 22.04616816910891 18.359424217206467 8 25.081102775627844 36.875903083379896 20.53122844615469 17.51176569483757 9 26.101058022137774 15.756897326241301 20.17660126999421 37.96544338162671 10 14.917778961570438 29.091606786629715 34.19425526952623 21.79635898227362 11 34.56044804041677 22.641758001036308 24.126481650341432 18.671312308205483 12 23.162745917287616 24.470921911938337 31.663036780506076 20.70329539026797 13 28.90314886720361 21.642636143709023 25.847419168173317 23.60679582091404 14 21.107899722464023 21.676260621103076 27.615576481707787 29.600263174725118 15 23.144516701752803 30.083452276411588 23.63256948069331 23.139461541142307 16 22.50201344749316 28.078468347609693 27.121434532031913 22.29808367286524 17 22.01840308242248 28.12940292042756 27.54714033010965 22.305053667040315 18 21.786095474367464 27.553650764229225 29.170612819504342 21.489640941898973 19 23.377973209946564 26.952929178348743 27.49333350694491 22.17576410475978 20 23.322749409944038 27.182441129399475 28.037337722642487 21.457471738014004 21 23.172164098424126 26.682404069563713 27.304151480417545 22.841280351594616 22 23.00136605377434 27.43734516046632 27.59294584690164 21.9683429388577 23 22.769395858417816 27.161325012178423 27.516643841701978 22.552635287701786 24 22.933711091988293 26.669056380785626 28.16572973788589 22.23150278934019 25 22.99726600063649 27.046985803040986 27.74639791083999 22.209350285482536 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 256.0 2 443.0 3 630.0 4 630.0 5 630.0 6 1669.0 7 2708.0 8 2708.0 9 2708.0 10 4070.5 11 5433.0 12 5433.0 13 5433.0 14 8825.5 15 12218.0 16 12218.0 17 12218.0 18 21261.5 19 30305.0 20 30305.0 21 30305.0 22 46977.0 23 63649.0 24 63649.0 25 63649.0 26 90903.5 27 118158.0 28 118158.0 29 118158.0 30 152536.5 31 186915.0 32 186915.0 33 186915.0 34 213226.0 35 239537.0 36 239537.0 37 239537.0 38 260005.5 39 280474.0 40 280474.0 41 280474.0 42 300142.5 43 319811.0 44 319811.0 45 319811.0 46 328561.0 47 337311.0 48 337311.0 49 337311.0 50 329049.5 51 320788.0 52 320788.0 53 320788.0 54 291459.5 55 262131.0 56 262131.0 57 262131.0 58 226571.0 59 191011.0 60 191011.0 61 191011.0 62 156808.0 63 122605.0 64 122605.0 65 122605.0 66 94810.5 67 67016.0 68 67016.0 69 67016.0 70 49149.0 71 31282.0 72 31282.0 73 31282.0 74 21882.5 75 12483.0 76 12483.0 77 12483.0 78 8485.5 79 4488.0 80 4488.0 81 4488.0 82 2944.5 83 1401.0 84 1401.0 85 1401.0 86 908.5 87 416.0 88 416.0 89 416.0 90 263.5 91 111.0 92 111.0 93 111.0 94 79.0 95 47.0 96 47.0 97 47.0 98 28.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.327906822666881 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5318668516651202E-4 22 8.425267684158161E-4 23 6.51043411957676E-4 24 7.659334258325601E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2611193.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.13388268254847 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8487824544074 60.59053136412611 2 11.758127584640695 17.198350466830995 3 3.0016563711763307 6.585683547088019 4 1.0520866445213715 3.0777272492920824 5 0.4710803033954612 1.7225965821291502 6 0.2431013072267233 1.0667365489616014 7 0.15097329354188696 0.7728884196663219 8 0.09507328463273704 0.5562462755660103 9 0.06644944420941136 0.43737352714184763 >10 0.28496807038581595 3.4951834422823396 >50 0.014247996229068633 0.7223947865136132 >100 0.011129500644345121 1.593011593966967 >500 0.0013731220388958317 0.7077415675088394 >1k 8.978105638213097E-4 1.238238753079662 >5k 5.281238610713586E-5 0.23529587584644873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6092 0.2333033215085978 No Hit GGTATCAACGCAGAGTACTTTTTTT 3493 0.1337702728216566 No Hit TATCAACGCAGAGTACTTTTTTTTT 3484 0.13342560278003196 No Hit GTCCTACAGTGGACATTTCTAAATT 3049 0.11676655076817379 No Hit CTGTAGGACGTGGAATATGGCAAGA 2878 0.11021781997730538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.8296671291628005E-5 0.0 0.0 0.0 2 0.0 3.8296671291628005E-5 0.0 0.0 0.0 3 0.0 3.8296671291628005E-5 0.0 0.0 0.0 4 0.0 3.8296671291628005E-5 0.0 0.0 0.0 5 0.0 3.8296671291628005E-5 0.0 0.0 0.0 6 7.659334258325601E-5 3.8296671291628005E-5 0.0 0.0 0.0 7 7.659334258325601E-5 3.8296671291628005E-5 0.0 0.0 0.0 8 7.659334258325601E-5 3.8296671291628005E-5 0.0 0.0 0.0 9 1.1489001387488401E-4 3.8296671291628005E-5 0.0 0.0 0.0 10 1.1489001387488401E-4 3.8296671291628005E-5 0.0 0.0 0.0 11 1.1489001387488401E-4 3.8296671291628005E-5 0.0 0.0 0.0 12 1.1489001387488401E-4 3.8296671291628005E-5 0.0 0.0 0.0 13 1.1489001387488401E-4 3.8296671291628005E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGCA 55 0.003176157 12.03583 6 TCCAACG 300 0.0 11.978518 18 TAGGACC 500 0.0 11.726337 4 CCAACGA 235 0.0 11.67 19 GTATCAA 2545 0.0 11.185586 1 AGGACGT 1510 0.0 10.834525 5 TTAGGAC 915 0.0 10.748626 3 TAGGACG 1580 0.0 10.71363 4 GTATACG 65 0.007933562 10.64483 1 GGACGTG 1540 0.0 10.623464 6 GTCCTAC 1595 0.0 10.597159 1 GTAGGAC 1560 0.0 10.487265 3 GGTATCA 1190 0.0 10.382862 1 TGTAGGA 1590 0.0 10.2574835 2 CGGTTTC 205 3.6379788E-12 10.14868 13 GTAGAAC 755 0.0 10.145625 3 GGCGAGG 730 0.0 10.1044445 19 GACGTGA 800 0.0 10.04777 7 CGAAATC 95 1.7223225E-4 9.954447 13 TAGAACA 915 0.0 9.818457 4 >>END_MODULE