FastQCFastQC Report
Thu 26 May 2016
SRR1511708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611193
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79700.3052244701942752No Hit
TATCAACGCAGAGTACTTTTTTTTT47650.18248363870460743No Hit
GGTATCAACGCAGAGTACTTTTTTT46580.17838589487640324No Hit
GTCCTACAGTGGACATTTCTAAATT32690.12519181845233193No Hit
CTGTAGGACGTGGAATATGGCAAGA32350.12388973162841659No Hit
GTCCTAAAGTGTGTATTTCTCATTT26980.10332441914481234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4950.011.5158484
TTAGGAC10700.011.2763823
AGGACGT18200.011.2754075
GGTATCA15150.011.2063971
GGACGTG18150.010.9360756
GGCGAGG7600.010.74694419
TAGGACG19650.010.6367754
TCCAACG2500.010.63677318
GCCGGTT1355.7178113E-810.55235311
GTCCTAC14600.010.3873061
GTCGAGG1106.0697002E-610.3606919
GTAGGAC17350.010.3493283
GACGTGA9650.010.136827
GTATCAA30300.010.1361241
CCAACGA1502.4576366E-810.13045319
TGTAGGA19600.010.0337712
GACGTGG10150.09.9181727
TAAGACA3850.09.8707284
AGTACCG1356.441678E-79.8524485
TATGTCG1356.4738197E-79.84886316
Produced by FastQC (version 0.11.5)