FastQCFastQC Report
Thu 26 May 2016
SRR1511707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256958
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51260.22711986665237016No Hit
GGTATCAACGCAGAGTACTTTTTTT30060.1331881231285651No Hit
TATCAACGCAGAGTACTTTTTTTTT28200.1249469418571369No Hit
GTCCTACAGTGGACATTTCTAAATT24670.10930642041189956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2500.013.61767919
TCCAACG3300.012.60896118
GCGTGCG1001.5323894E-712.2934669
CGTGCGC1251.5170372E-912.10433610
GGTATCA10750.011.8631941
ACTGTTC8500.011.3478158
TGGACCG855.579803E-511.1253095
TAGAACG600.00608118111.0325984
CGTTTCC3000.011.03259814
CCCGTTT2900.010.76085912
GCGTTGA803.9354182E-410.638576514
ATCCCGT3200.010.638576510
GATATAC8000.010.6274451
TGTAGGA12950.010.6112232
GGGCTAT2051.8189894E-1210.6093611
TCCCGTT2950.010.5784711
GTCCTAC12450.010.5609431
GTAGGAC13350.010.5544593
AGGCGTG1553.952664E-910.3716587
TCCTACA14300.010.3007922
Produced by FastQC (version 0.11.5)