##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511707_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256958 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.582070202458354 31.0 30.0 34.0 25.0 34.0 2 26.903818325374242 31.0 26.0 34.0 10.0 34.0 3 28.581515030408188 31.0 28.0 34.0 16.0 34.0 4 33.09208766844576 35.0 33.0 37.0 27.0 37.0 5 33.54213591923288 35.0 35.0 37.0 28.0 37.0 6 33.79426954334108 37.0 35.0 37.0 29.0 37.0 7 33.84355535193831 37.0 35.0 37.0 29.0 37.0 8 33.86718273002865 37.0 35.0 37.0 30.0 37.0 9 35.47775811512665 39.0 35.0 39.0 30.0 39.0 10 35.35985162329117 39.0 35.0 39.0 30.0 39.0 11 35.38108994496132 39.0 35.0 39.0 30.0 39.0 12 35.28086521769568 39.0 35.0 39.0 29.0 39.0 13 35.27992855870601 39.0 35.0 39.0 30.0 39.0 14 36.44358158193462 40.0 37.0 41.0 29.0 41.0 15 36.387605795056885 40.0 36.0 41.0 29.0 41.0 16 36.322237276901035 40.0 36.0 41.0 29.0 41.0 17 36.29512955048344 40.0 36.0 41.0 29.0 41.0 18 36.234403564443824 40.0 36.0 41.0 29.0 41.0 19 36.26712282638844 40.0 37.0 41.0 29.0 41.0 20 36.18918650679366 40.0 37.0 41.0 28.0 41.0 21 36.11446291867195 40.0 36.0 41.0 28.0 41.0 22 36.01071397872712 40.0 36.0 41.0 27.0 41.0 23 35.908282741637194 40.0 36.0 41.0 27.0 41.0 24 35.796568212611845 40.0 36.0 41.0 27.0 41.0 25 35.69122509147268 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7812835526529156 1101 2 3.3576873507319185 1101 3 1.901555936418788 1101 4 1.0160011610502977 1101 5 0.5711710134968158 1101 6 0.28595005738041124 1101 7 0.3333318586072238 1101 8 0.046213868288766946 1101 9 0.025676992537462695 1101 10 0.224852936865787 1101 11 0.08896491845661814 1101 12 0.12920350822292193 1101 13 0.17692935328972226 1101 14 0.3796787974093192 1101 15 0.17997431994407265 1101 16 0.1811835542184781 1101 17 0.3161704633036919 1101 18 0.26290607808044086 1101 19 0.2443880495716826 1101 20 0.26708967426583285 1101 21 0.3681207407110776 1101 22 0.2953712110255111 1101 23 0.3738655853601358 1101 24 0.2539986995372061 1101 25 0.26829768787102637 1102 1 1.4605457660128351 1102 2 4.177143268692323 1102 3 2.6807896638080493 1102 4 1.7857917891659483 1102 5 1.386567680638251 1102 6 1.0545585177136942 1102 7 0.9718448909387973 1102 8 1.0272564548149035 1102 9 1.0678932010547655 1102 10 0.960674044970041 1102 11 1.1457801869368964 1102 12 1.1027827704362778 1102 13 1.1739240833851454 1102 14 1.254696601140978 1102 15 1.2082324028382487 1102 16 1.272808678844605 1102 17 1.2861889791846224 1102 18 1.2767787457915958 1102 19 1.2298459615500335 1102 20 1.1891520585415805 1102 21 1.164360592584032 1102 22 1.052499825183034 1102 23 1.1138314021953448 1102 24 0.970449347593032 1102 25 0.8443694725170872 1103 1 1.484090154513197 1103 2 3.98403507693223 1103 3 2.5924673139856544 1103 4 1.5526932033634182 1103 5 1.197134000798755 1103 6 0.8670728421315985 1103 7 0.8553330193042328 1103 8 0.6064625877058631 1103 9 0.6654975405227788 1103 10 0.5903035277988096 1103 11 0.7468806752785966 1103 12 0.8635524594434045 1103 13 0.8510060639377457 1103 14 0.9482525657528171 1103 15 0.8180610227629188 1103 16 0.7193343881874483 1103 17 0.9494768513134701 1103 18 0.9110862366898331 1103 19 0.9485075159198644 1103 20 0.8516695888312427 1103 21 1.0518864872845484 1103 22 1.0476574433252424 1103 23 1.029317861175926 1103 24 1.2340417192503281 1103 25 1.0240549706687574 1104 1 1.4105493654081371 1104 2 4.040340194465937 1104 3 2.560919516278549 1104 4 1.6932217409340566 1104 5 1.1588461664240626 1104 6 0.9626119076013353 1104 7 0.8925724944157025 1104 8 0.878389121669727 1104 9 0.9157029975803823 1104 10 0.8426145646770209 1104 11 0.9266606561609194 1104 12 0.8819398720432119 1104 13 0.8442151316318984 1104 14 0.9709876928090111 1104 15 0.881079593081715 1104 16 0.8768448007761194 1104 17 0.9213565567458772 1104 18 0.6904431930216006 1104 19 0.9426681502794665 1104 20 0.8600912264756531 1104 21 0.9915247147257986 1104 22 1.1035808435719474 1104 23 1.0201995894827363 1104 24 1.0616084328667057 1104 25 0.9115853802445884 1105 1 1.5087804898854955 1105 2 3.875719968745038 1105 3 2.3702927150128943 1105 4 1.640188561947241 1105 5 1.195003390903338 1105 6 0.898039526978998 1105 7 0.8224944446498341 1105 8 0.7632707235532337 1105 9 0.9003845120453562 1105 10 0.6956550140291284 1105 11 0.7186979518288297 1105 12 0.812700526393499 1105 13 0.7394539566015581 1105 14 0.7653869864951943 1105 15 0.8067581750209101 1105 16 0.8447909791760821 1105 17 0.9489671838431804 1105 18 0.9141571219966593 1105 19 0.883712235684925 1105 20 0.8462107060045696 1105 21 1.0061618756089672 1105 22 0.9952682910032848 1105 23 1.0835782370728992 1105 24 0.9899596359162572 1105 25 1.0121238661627174 1106 1 1.0104608433278628 1106 2 3.53377911718621 1106 3 2.1730644796825267 1106 4 1.0553080837061302 1106 5 0.6058878442155162 1106 6 0.5630290500475041 1106 7 0.3998754428857012 1106 8 0.15796889548276027 1106 9 0.2109687165304095 1106 10 0.1965245608248054 1106 11 0.34926959940334257 1106 12 0.35153546850074946 1106 13 0.49061720414217547 1106 14 0.6708480870352247 1106 15 0.5342809199769576 1106 16 0.5358283879844095 1106 17 0.46326415092558193 1106 18 0.49454831212985795 1106 19 0.38366697688160656 1106 20 0.4665403826833838 1106 21 0.4356592525242817 1106 22 0.3213579689146826 1106 23 0.5645820580714371 1106 24 0.44445239065591124 1106 25 0.3111487796895176 1107 1 1.404751200752699 1107 2 3.8749888106213923 1107 3 2.354877179187003 1107 4 1.5228715013056373 1107 5 1.1703713243970597 1107 6 1.0712368883029555 1107 7 0.8591007237056587 1107 8 0.7755550789953958 1107 9 0.8902512511999916 1107 10 0.8112788100582691 1107 11 0.9180042505762742 1107 12 0.7373024133636434 1107 13 0.790742901045526 1107 14 0.8822455750346805 1107 15 1.032179190251945 1107 16 0.9555252622729711 1107 17 0.8666376233085913 1107 18 0.8864886945003221 1107 19 0.9613774525923162 1107 20 1.0976749486052313 1107 21 1.0376481214459048 1107 22 1.0567076194336948 1107 23 1.14770161786587 1107 24 1.1999668276355067 1107 25 1.0791958518477713 1108 1 1.5631950995655757 1108 2 4.190962459210542 1108 3 2.821803578312174 1108 4 1.6477990594996967 1108 5 1.3897228601633458 1108 6 1.2375550834319995 1108 7 0.9354729199773928 1108 8 0.9301410804739234 1108 9 0.9123351997340521 1108 10 0.837837460787398 1108 11 0.9762681455801925 1108 12 1.0150675401342468 1108 13 1.0819173900620171 1108 14 0.9536765745984894 1108 15 1.0636098212072724 1108 16 1.063747688822609 1108 17 1.044295444054292 1108 18 0.9892231550930006 1108 19 1.033378976174042 1108 20 1.1728542868710932 1108 21 1.0017630617620483 1108 22 1.1951674093678193 1108 23 1.3556871110742605 1108 24 1.299029538177507 1108 25 1.247817645209956 1109 1 1.0394502163613097 1109 2 3.213964805817813 1109 3 1.9586144612351362 1109 4 1.251246575480046 1109 5 0.987016616072502 1109 6 0.7543325780771113 1109 7 0.5336176707322906 1109 8 0.48190440847888283 1109 9 0.5614292667612517 1109 10 0.4975905209004807 1109 11 0.38190697807888085 1109 12 0.4176704997797316 1109 13 0.37233293845260107 1109 14 0.5290035110668683 1109 15 0.5853543587248211 1109 16 0.4612320323186978 1109 17 0.6240975288287842 1109 18 0.4705363353762877 1109 19 0.6989113662910853 1109 20 0.6706124970263829 1109 21 0.6508682428854087 1109 22 0.7433481659618266 1109 23 0.7067087839450181 1109 24 0.625774384207034 1109 25 0.5657189282748689 1110 1 0.8403388354347072 1110 2 3.3908908225083714 1110 3 1.895946053738065 1110 4 1.1288104216247419 1110 5 0.48859511106712006 1110 6 0.3131023334289793 1110 7 0.26134661570579 1110 8 0.18086727109405132 1110 9 0.15992237283492727 1110 10 0.1814809125278387 1110 11 0.15985797558660408 1110 12 0.28718461626864666 1110 13 0.1907299890536578 1110 14 0.254010425811785 1110 15 0.10215384301868369 1110 16 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-1.8849209494015113 2315 10 -2.0381303499689096 2315 11 -1.9452719689459244 2315 12 -2.2068625400038187 2315 13 -1.968423386407082 2315 14 -2.258800813998363 2315 15 -2.258026897839123 2315 16 -2.5478747119951635 2315 17 -2.255318827662265 2315 18 -2.2599399725284357 2315 19 -2.3483530794788336 2315 20 -2.4139858190161334 2315 21 -2.4791272436573664 2315 22 -2.308058544367782 2315 23 -2.2362658736309555 2315 24 -2.1934446050855883 2315 25 -2.1824691036784074 2316 1 -0.3497533140369491 2316 2 2.003270661360215 2316 3 0.5086642103856853 2316 4 -0.26603463919468595 2316 5 -0.6383129494515813 2316 6 -0.6034427030212015 2316 7 -1.1290636950799353 2316 8 -1.1424563058972268 2316 9 -1.1421001453901596 2316 10 -1.075213178187994 2316 11 -1.232428961976268 2316 12 -1.3092959064777148 2316 13 -1.1947776302260493 2316 14 -1.4364281429354335 2316 15 -1.3391337608098794 2316 16 -1.5026657306294737 2316 17 -1.255622107507719 2316 18 -1.6818229445413237 2316 19 -1.6898535103813117 2316 20 -1.5315386647896716 2316 21 -1.7132015301749774 2316 22 -1.5337432623597849 2316 23 -1.7993807618963515 2316 24 -1.8216354879835066 2316 25 -1.8795671959030855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68646.0 3 2.0 4 603.0 5 3083.0 6 3716.0 7 3412.0 8 3057.0 9 3050.0 10 2914.0 11 2923.0 12 2713.0 13 2778.0 14 2862.0 15 2912.0 16 3200.0 17 3361.0 18 3853.0 19 4725.0 20 6159.0 21 8023.0 22 9773.0 23 11723.0 24 13600.0 25 15964.0 26 17983.0 27 20339.0 28 24038.0 29 29670.0 30 37212.0 31 48330.0 32 62967.0 33 84100.0 34 115487.0 35 167526.0 36 269620.0 37 343378.0 38 853256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96058765825505 18.09404517053485 12.814017806268438 25.131349364941663 2 16.72022759612249 20.56461773337681 39.60772833723654 23.107426333264165 3 19.626373197906208 23.335746611146508 29.130847804877185 27.907032386070103 4 12.433106863308932 16.23198127745399 37.859277842122005 33.47563401711508 5 13.632154430875543 37.17366472925061 35.59206684395545 13.6021139959184 6 32.575617268907976 36.559120727988734 17.705380427992015 13.15988157511128 7 28.569029640781974 31.109440228839 21.384004487456124 18.93752564292291 8 25.21801469057023 37.21376294995299 20.034843359956188 17.533378999520593 9 25.999819225701142 15.911018282130197 19.790709441646676 38.298453050521985 10 14.748081266908821 29.414991329036695 34.04143984956743 21.79548755448706 11 35.279876719017366 22.57215242817988 23.424405770953648 18.72356508184911 12 22.977122303560808 24.39464978967265 31.582289081143735 21.045938825622805 13 29.05565810263195 21.435711253820408 25.658120354920204 23.850510288627436 14 21.043812069165664 21.553125933225164 27.46861040391536 29.93445159369381 15 23.309560922267938 29.949516118598574 23.494278582056026 23.246644377077462 16 22.626783484672732 28.26127025846294 26.870814609753484 22.241131647110844 17 22.007409973956094 28.044917096374856 27.485713070424882 22.461959859244168 18 21.74914198669182 27.8442487631582 28.858844515493864 21.547764734656116 19 23.285911074070086 26.892847316098624 27.41102289498648 22.410218714844813 20 23.096397894865568 27.387616428839173 27.96002406779391 21.555961608501352 21 23.298958109488225 26.697563752932602 27.120833157949537 22.882644979629635 22 22.791104069134253 27.325115133663157 27.665088424957307 22.21869237224528 23 22.93109254122839 26.992122076794246 27.285572500819693 22.791212881157673 24 22.85011316126677 26.764722041546225 28.055531432602145 22.329633364584865 25 23.061660872732233 26.851053497672535 27.73223958974868 22.355046039846556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 305.5 3 440.0 4 440.0 5 440.0 6 1209.0 7 1978.0 8 1978.0 9 1978.0 10 3136.5 11 4295.0 12 4295.0 13 4295.0 14 7016.0 15 9737.0 16 9737.0 17 9737.0 18 17044.5 19 24352.0 20 24352.0 21 24352.0 22 38551.0 23 52750.0 24 52750.0 25 52750.0 26 74538.0 27 96326.0 28 96326.0 29 96326.0 30 127983.5 31 159641.0 32 159641.0 33 159641.0 34 180766.0 35 201891.0 36 201891.0 37 201891.0 38 221505.5 39 241120.0 40 241120.0 41 241120.0 42 258202.0 43 275284.0 44 275284.0 45 275284.0 46 283139.0 47 290994.0 48 290994.0 49 290994.0 50 285988.0 51 280982.0 52 280982.0 53 280982.0 54 256192.5 55 231403.0 56 231403.0 57 231403.0 58 200762.5 59 170122.0 60 170122.0 61 170122.0 62 139902.0 63 109682.0 64 109682.0 65 109682.0 66 84916.5 67 60151.0 68 60151.0 69 60151.0 70 44236.0 71 28321.0 72 28321.0 73 28321.0 74 19816.5 75 11312.0 76 11312.0 77 11312.0 78 7720.0 79 4128.0 80 4128.0 81 4128.0 82 2732.0 83 1336.0 84 1336.0 85 1336.0 86 863.0 87 390.0 88 390.0 89 390.0 90 254.5 91 119.0 92 119.0 93 119.0 94 73.0 95 27.0 96 27.0 97 27.0 98 16.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.344165908271222 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.430742619047408E-5 20 0.0 21 1.3292227857142225E-4 22 7.089188190475853E-4 23 7.975336714285333E-4 24 8.861485238094816E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2256958.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.7286782173267 #Duplication Level Percentage of deduplicated Percentage of total 1 86.18488285435596 66.12852143729876 2 9.766433915877895 14.987311305243619 3 2.1648045440854933 4.9830777379962745 4 0.7592152395844477 2.3301432726306275 5 0.3645371832345484 1.3985228115327164 6 0.20385422469606954 0.93848791259684 7 0.13094336825583017 0.7032978097316155 8 0.08859749800081385 0.5438375131971752 9 0.05419911417484158 0.37427637520670126 >10 0.2572767327094374 3.350672731285849 >50 0.013956642915753667 0.7452213213902783 >100 0.009378984148044707 1.539651156065169 >500 9.889353132013596E-4 0.5649020446556419 >1k 8.725899822354326E-4 1.1832769598457784 >5k 5.817266548236218E-5 0.22879961132287666 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5126 0.22711986665237016 No Hit GGTATCAACGCAGAGTACTTTTTTT 3006 0.1331881231285651 No Hit TATCAACGCAGAGTACTTTTTTTTT 2820 0.1249469418571369 No Hit GTCCTACAGTGGACATTTCTAAATT 2467 0.10930642041189956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.430742619047408E-5 2 0.0 0.0 0.0 0.0 4.430742619047408E-5 3 0.0 0.0 0.0 0.0 4.430742619047408E-5 4 0.0 0.0 0.0 0.0 4.430742619047408E-5 5 0.0 0.0 0.0 0.0 4.430742619047408E-5 6 0.0 0.0 0.0 0.0 4.430742619047408E-5 7 0.0 0.0 0.0 0.0 4.430742619047408E-5 8 0.0 0.0 0.0 4.430742619047408E-5 4.430742619047408E-5 9 0.0 0.0 0.0 4.430742619047408E-5 4.430742619047408E-5 10 0.0 0.0 0.0 8.861485238094816E-5 4.430742619047408E-5 11 0.0 0.0 0.0 8.861485238094816E-5 4.430742619047408E-5 12 0.0 0.0 0.0 8.861485238094816E-5 1.3292227857142225E-4 13 0.0 0.0 0.0 8.861485238094816E-5 1.3292227857142225E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 250 0.0 13.617679 19 TCCAACG 330 0.0 12.608961 18 GCGTGCG 100 1.5323894E-7 12.293466 9 CGTGCGC 125 1.5170372E-9 12.104336 10 GGTATCA 1075 0.0 11.863194 1 ACTGTTC 850 0.0 11.347815 8 TGGACCG 85 5.579803E-5 11.125309 5 TAGAACG 60 0.006081181 11.032598 4 CGTTTCC 300 0.0 11.032598 14 CCCGTTT 290 0.0 10.760859 12 GCGTTGA 80 3.9354182E-4 10.6385765 14 ATCCCGT 320 0.0 10.6385765 10 GATATAC 800 0.0 10.627445 1 TGTAGGA 1295 0.0 10.611223 2 GGGCTAT 205 1.8189894E-12 10.609361 1 TCCCGTT 295 0.0 10.57847 11 GTCCTAC 1245 0.0 10.560943 1 GTAGGAC 1335 0.0 10.554459 3 AGGCGTG 155 3.952664E-9 10.371658 7 TCCTACA 1430 0.0 10.300792 2 >>END_MODULE