FastQCFastQC Report
Thu 26 May 2016
SRR1511707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256958
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63270.28033308550712954No Hit
GGTATCAACGCAGAGTACTTTTTTT39220.17377372551903933No Hit
TATCAACGCAGAGTACTTTTTTTTT39160.17350788096189648No Hit
GTCCTACAGTGGACATTTCTAAATT26620.117946368519042No Hit
CTGTAGGACGTGGAATATGGCAAGA23770.1053187520547569No Hit
TTGTAGAACAGTGTATATCAATGAG22610.1001790906166619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2400.013.05911819
TCCAACG3250.012.27320118
TAGGACC5350.011.8980694
ACCGTGC907.4898653E-611.6075928
GGTATCA13750.011.514081
GTTCACG600.005708242811.1268081
ACCGTCC600.00589033111.0799738
TTCGAAA700.001495080510.85433219
ACTGTTC10400.010.3189868
ACTGCGT1201.530314E-610.2885478
TGTAGAA11000.010.27802852
GATATAC8950.010.2299161
AAACGGG750.002654059610.1307119
ACCGCTC856.613815E-410.0557748
AGGACGT12550.09.7656575
TGTAGGA14800.09.7574742
GACGTGG6750.09.7081677
GTATCAA25650.09.7045851
GGACGTG12300.09.6515456
TAGAACA11450.09.6251614
Produced by FastQC (version 0.11.5)